Structural and Functional Alterations of Hepatocytes during Transient Phalloidin-Induced Cholestasis in the Rat

Loranger, Anne; Barriault, Claude; Yousef, Ibrahim M.; Tuchweber, Béatriz

doi:10.1006/taap.1996.0061

Cited by 18 publications

(9 citation statements)

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“…The same has been observed in other similar experiments with cholestatic liver damage in rats -alpha-naphthylisothiocyanateinduced liver injury 11,12 ; parenteral nutrition-associated cholestasis 13 ; bile duct ligation 14,15,16 ; cyclosporine induced hepatotoxicity in the isolated perfused rat liver 17 ; falloidin-induced transient cholestasis 18 . Also in models of hepatocyte damage In conclusion, TFB was able to ameliorate liver injury and protect rats from CCl 4 -induced liver fibrosis by suppressing oxidative stress.…”

Section: Also Called C Forskohlii)supporting

confidence: 79%

“…Leaves of Bidens pilosa were dried in an oven at 60 o C for 72 hours, ground with pestle and mortar, and suspended in water in the proportion of 60 grams per one liter of water. The suspension was filtered through a paper filter and stored in aliquots of 100 ml at minus 20 o Cesius, until use 100g of wet leaves produced in average, 18.76g of dried leaves.…”

Section: Collection and Preparation Of Water Extract Of Bidens Pilosamentioning

confidence: 99%

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An acqueous extract of Bidens pilosa L. protects liver from cholestatic disease: experimental study in young rats

et al. 2009

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Purpose:To test the hepatoprotective effect of water extract from Bidens Pilosa L. (BPE) in cholestatic liver disease induced by ligature and resection of the common bile ducts (LRBD) in young rats. Methods: We studied four groups of ten 21 days old (P21) Wistar rats, Group SW: sham operation and water; Group SD: sham operation and BPE (160 mg of fresh leaves/100 g of body weight/day); Group LW: LRBD and water and Group LD: LRBD and BPE daily. Pentobarbital sleeping time (PST) and serum activities of aspartate aminotransferase (AST) and of alanine aminotransferase (ALT) were determined after the sacrifice (P70). A Ruwart's score for hepatic fibrosis (RS) was given to each animal. Were employed two way ANOVA and the test of Tukey or a non-parametric test for multiple comparisons. Results: There were statistically significant differences between LW and LD in the measurements of the PST ((means LW=390; LD=173), AST (means LW=8, LD=5), ALT (medians LW=2; LD=1) e RS (medians LW=2; LD=1). Conclusion: BPE could be used in the phytotherapy of the hepatic damage induced by chronic obstructive cholestasis, because protects liver function, decreases the rate of necrosis and liver fibrosis in cholestatic liver disease. Key words: Bidens. Phytotherapy. Cholestasis, Extrahepatic. Liver Cirrhosis, Biliary. Rats. RESUMO Objetivo:Testar o efeito hepatoprotetor do extrato aquoso de Bidens pilosa L. (EBP) na doença hepática induzida pela ligadura e ressecção do ducto biliar comum (LRDBC) em ratos jovens. Métodos: Estudamos ratos Wistar com 21º. dia de vida (P21) divididos em quatro grupos de 10 animais, Grupo SA: operação simulada e água; Grupo SD: operação simulada e EBP (160mg de folhas frescas/100g de peso corporal/dia); Grupo LA: LRDBC e água e Grupo LD: LRDBC e EBP diariamente. O tempo de sono por pentobarbital (TSP), aspartato (AST) e alanina (ALT) aminotransferase foram determinadas após o sacrifício (P70). O Score de Ruwart (SR) para fibrose hepática foi atribuído para cada animal. Foi realizada análise de variância com dois fatores e pelo teste de Tukey para comparações múltiplas ou por teste não paramétrico. Resultados: Houve diferença estatisticamente significativa entre LA e LD nas variáveis: TSP (médias LA=390; LD=173), AST (médias LA=8, LD=5), ALT (medianas LA=2; LD=1) e SR (medianas LA=2; LD=1). Conclusão: EBP poderá ser empregado na fitoterapia da doença hepática induzida pela colestase obstrutiva crônica, pois protege a função hepática, diminui a velocidade de necrose e a intensidade da fibrose hepática na obstrução biliar extra-hepática.

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Section: Also Called C Forskohlii)supporting

confidence: 79%

Section: Collection and Preparation Of Water Extract Of Bidens Pilosamentioning

confidence: 99%

An acqueous extract of Bidens pilosa L. protects liver from cholestatic disease: experimental study in young rats

et al. 2009

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“…Because these lesions appear to be devoid of microfilamentous support, they may be prone to mechanical separation during homogenization. 40 Based on previous studies 5,38 and the findings presented here we propose the following pathogenetic scheme for phalloidin-induced cholestasis. After intravenous administration, phalloidin is preferentially transported into the hepatocytes 41 where it binds to actin and stabilizes actin filaments.…”

Section: H]leukotrienes In the Liver After Intravenous [ 3 H]ltc 4 Inmentioning

confidence: 60%

“…1 Cholestasis is among the earliest effects of acute phalloidin poisoning 2,3 and has been extensively studied in rats. [3][4][5][6][7] Phalloidin-induced cholestasis involves impaired secretion of bile acids and organic anions, 7,8 although disruption of actin organization by itself does not seem to decrease canalicular secretion of bile salt and nonbile salt organic anions in isolated rat hepatocyte couplets. 9 Rather, the cholestatic impact of phalloidin has been attributed to a dysfunction of pericanalicular microfilamentous structures with alteration of the actomyosin complex and a loss of canalicular contractility.…”

mentioning

confidence: 99%

Changes in the localization of the rat canalicular conjugate export pump mrp2 in phalloidin-induced cholestasis

1999

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Administration of phalloidin, one of the toxic peptides of the mushroom Amanita phalloides, leads to rapid and sustained cholestasis in rats. Although attributed to the interaction of phalloidin with microfilaments, the events leading to cholestasis are incompletely understood. The adenosine triphosphate (ATP)-dependent, apical conjugate export pump, termed multidrug resistance protein 2 (Mrp2) or canalicular multispecific organic anion transporter, is the major driving force for bile salt-independent bile flow. We

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“…Glutathione is considered to be a driving force for the bile acid independent canalicular bile flow [30]. Accordingly, the impaired biliary excretion of glutathione seems to be the cause of the decrease in the bile acid independent canalicular bile flow in phalloidin-treated rats [7,31].…”

Section: Discussionmentioning

confidence: 99%

Effects of Phalloidin on the Biliary Excretion of Cholephilic Compounds in Rats

Ohashi¹,

Sano²,

Takikawa³

2002

Pharmacology

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Phalloidin is known to cause cholestasis by preventing microfilament depolymerization. In addition, phalloidin is reported to inhibit the vesicular targeting of canalicular transporters. The aim of the present study was to examine the effect of phalloidin on the biliary excretion of substrates typical for various canalicular transporters in rats. Phalloidin decreased the biliary excretion of tracer amounts of taurocholate, leukotriene C₄, pravastatin, and vinblastine. Increases in bile flow and biliary bile acid excretion caused by taurocholate infusion were completely inhibited by phalloidin. These data indicate that, in addition to multidrug resistance protein 2 and P glycoprotein, the vesicular targeting of the bile salt export pump, a major canalicular bile acid transporter, is also impaired by phalloidin. The decrease of biliary excretion of glutathione may also relate to the increase in the bile acid independent canalicular bile flow in phalloidin-treated rats.

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Structural and Functional Alterations of Hepatocytes during Transient Phalloidin-Induced Cholestasis in the Rat

Cited by 18 publications

References 0 publications

An acqueous extract of Bidens pilosa L. protects liver from cholestatic disease: experimental study in young rats

An acqueous extract of Bidens pilosa L. protects liver from cholestatic disease: experimental study in young rats

Changes in the localization of the rat canalicular conjugate export pump mrp2 in phalloidin-induced cholestasis

Effects of Phalloidin on the Biliary Excretion of Cholephilic Compounds in Rats

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