Structural and electronic effects on complex formation of copper(II) and nickel(II) with sulfhydryl-containing peptides

Hirayama, Yoshinobu

doi:10.1021/ic50157a037

Cited by 35 publications

(14 citation statements)

References 17 publications

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“…They usually target sulfhydryl groups of enzymes [42]. The heavy metal mercury targets sulfhydryl groups and also carboxyl, amide, phosphoryl and amine groups of enzymes.…”

Section: +mentioning

confidence: 99%

Assessing Resistance and Bioremediation Ability of Enterobacter sp. Strain Saw-1 on Molybdenum in Various Heavy Metals and Pesticides

Sabullah

Rahman

Ahmad

et al. 2017

J. Math. Fund. Sci.

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Abstract. One of the most economical approaches for removal of toxic compounds is bioremediation. In the long term, bioremediation is economic and feasible compared to other methods, such as physical or chemical methods. A bacterium that can efficiently reduce molybdenum blue was isolated from polluted soil. Biochemical analysis revealed the identity of the bacterium as Enterobacter sp. strain Saw-1. The growth parameters for optimal reduction of molybdenum to Mo-blue or molybdenum blue, a less toxic product, were determined around pH 6.0 to 6.5 and in the range of 30 to 37 ℃, respectively. Glucose was selected as preferred carbon source, followed by sucrose, maltose, l-rhamnose, cellobiose, melibiose, raffinose, d-mannose, lactose, glycerol, dadonitol, d-mannitol, l-arabinose and mucate. Phosphate and molybdate were critically required at 5.0 mM and 10 mM, respectively. The scanning absorption spectrum acquired to detect the development of complex Mo-blue showed similarity to previously isolated Mo-reducing bacteria. In addition, the spectrum closely resembled the molybdenum blue from the phosphate determination method. Heavy metals, including mercury, copper (II) and silver (I), inhibited reduction. Moreover, the bacterium also showed capability of exploiting the pesticide coumaphos as an alternative carbon source for growth. As the bacterium proved its ability to detoxify organic and inorganic xenobiotics, the usefulness of this microorganism for bioremediation is highlighted.

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“…They usually target sulfhydryl groups of enzymes [42]. The heavy metal mercury targets sulfhydryl groups and also carboxyl, amide, phosphoryl and amine groups of enzymes.…”

Section: +mentioning

confidence: 99%

Assessing Resistance and Bioremediation Ability of Enterobacter sp. Strain Saw-1 on Molybdenum in Various Heavy Metals and Pesticides

Sabullah

Rahman

Ahmad

et al. 2017

J. Math. Fund. Sci.

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“…Therefore, the screening and isolation of molybdenum-reducing bacteria with as many metal resistance capability is important. Heavy metals for example cadmium, silver, mercury, and copper usually target the sulfhydryl group of enzymes (Sugiura and Hirayama, 1976;Sabullah et al, 2014;Sabullah et al, 2015), while chromate is known to inhibit the enzyme glucose oxidase (Zeng et al, 2004). Binding of these heavy metals leads to the inactivation of the metal-reducing capacity of the enzyme(s) responsible for molybdenum reduction.…”

Section: Effect Of Heavy Metalsmentioning

confidence: 99%

ISOLATION AND CHARACTERIZATION OF A MOLYBDENUM-REDUCING AND GLYPHOSATE-DEGRADING Klebsiella oxytoca STRAIN SAW-5 IN SOILS FROM SARAWAK

et al. 2016

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Bioremediation of pollutants including heavy metals and xenobiotics is an economic and environmentally friendly process. A novel molybdenum-reducing bacterium with the ability to utilize the pesticide glyphosate as a carbon source is reported. The characterization works were carried out utilizing bacterial resting cells in a microplate format. The bacterium reduces molybdate to Mo-blue optimally between pH 6.3 and 6.8 and at 34 o C. Glucose was the best electron donor for supporting molybdate reduction followed by lactose, maltose, melibiose, raffinose, d-mannitol, d-xylose, l-rhamnose, l-arabinose, dulcitol, myo-inositol and glycerol in descending order. Other requirements include a phosphate concentration at 5.0 mM and a molybdate concentration between 20 and 30 mM. The molybdenum blue exhibited an absorption spectrum resembling a reduced phosphomolybdate. Molybdenum reduction was inhibited by mercury, silver, cadmium and copper at 2 ppm by 45.5, 26.0, 18.5 and 16.3%, respectively. Biochemical analysis identified the bacterium as Klebsiella oxytoca strain Saw-5. To conclude, the capacity of this bacterium to reduce molybdenum into a less toxic form and to grow on glyphosate is novel and makes the bacterium an important instrument for bioremediation of these pollutants.

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“…Equilibrium species for thiorphan and tiopronin in the presence of Cu(II). The p K a values were from Sugiura and Hirayama [44] and Sigel and Martin [63].…”

Section: Resultsmentioning

confidence: 99%

“…, (a) the first Cu(II) binds with a sufficiently low K d that the [Cu(II)] free was reduced from 1.0 to 0.77 lM, (b) that [PAM] copper-free is Equilibrium species for thiorphan and tiopronin in the presence of Cu(II). The pK a values were from Sugiura and Hirayama[44] and Sigel and Martin[63].…”

mentioning

confidence: 99%

Thiorphan, tiopronin, and related analogs as substrates and inhibitors of peptidylglycine α‐amidating monooxygenase (PAM)

et al. 2005

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Peptidyglycine a-amidating monooxygenase is a copper-and zinc-dependent, bifunctional enzyme that catalyzes the cleavage of glycine-extended peptides or N-acylglycines to the corresponding amides and glyoxylate. This reaction is a key step in the biosynthesis of bioactive a-amidated peptides and, perhaps, the primary fatty acids amides also. Two clinically useful N-acylglycines are thiorphan and tiopronin, each with a thiol moiety attached to the acyl group. We report here that thiorphan and tiopronin are substrates for PAM, exhibiting relatively low K M,app and V MAX,app values. The low V MAX,app values result, most likely, from a decrease in active PAM AE 2Cu(II) as the enzyme competes ineffectively with thiorphan and tiopronin for free copper.

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Structural and electronic effects on complex formation of copper(II) and nickel(II) with sulfhydryl-containing peptides

Cited by 35 publications

References 17 publications

Assessing Resistance and Bioremediation Ability of Enterobacter sp. Strain Saw-1 on Molybdenum in Various Heavy Metals and Pesticides

Assessing Resistance and Bioremediation Ability of Enterobacter sp. Strain Saw-1 on Molybdenum in Various Heavy Metals and Pesticides

ISOLATION AND CHARACTERIZATION OF A MOLYBDENUM-REDUCING AND GLYPHOSATE-DEGRADING Klebsiella oxytoca STRAIN SAW-5 IN SOILS FROM SARAWAK

Thiorphan, tiopronin, and related analogs as substrates and inhibitors of peptidylglycine α‐amidating monooxygenase (PAM)

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