Structural and Distribution Patterns of Surface ‘Prismless’ Enamel in Human Permanent Teeth

Kodaka, Tetsuo; Kuroiwa, Mie; Higashi, Shohei

doi:10.1159/000261336

Cited by 58 publications

(57 citation statements)

References 15 publications

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“…All teeth were from per sons who had been living in an area with fluoridated water (1 ppm F). But because a natural, nonpolished enamel sur face is known to have irregularities in its structure [Whittak er, 1982;Fejerskov et al, 1984;Kodaka et al, 1991], the sur face variations observed are understandable. It would have been impossible to evaluate the degree of erosion on the basis o f the scanning electron microscopic pictures alone.…”

Section: Scanning Electron Microscopical Findingsmentioning

confidence: 99%

Effect of Fluoride Varnish and Solution on Enamel Erosion in vitro

Sorvari¹,

et al. 1994

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The effect of fluoride varnish and solution on initial enamel erosion was studied. Enamel specimens prepared from human third molars were treated for 24 h with Duraphat® varnish (2.26% F) or for 48 h with NaF solution (1.2% F), washed, and immersed in cola beverage (pH 2.6) for up to 15 min. The surface microhardness was measured using a Vickers diamond in a Leitz indentation apparatus at baseline, after fluoride treatment, and after 1, 5, and 15 min exposure to acidic drink. The specimens were then prepared and studied using a JEOL JSM–35 scanning electron microscope. The results showed that both fluoride treatments caused an increase in enamel hardness values and subsequent inhibition of softening which was statistically highly significant. Scanning electron micrographs revealed the difficulty of removing Duraphat varnish from the enamel surface, and remnants of varnish were seen irrespective of vigorous sonication in acetone. However, in areas devoid of varnish, erosion was observed as mainly irregular lesions similar to those seen in the NaF solution treated and control specimens. The present study showed that treatment of enamel with topical fluoride prior to acidic challenge can inhibit initial erosion.

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Section: Scanning Electron Microscopical Findingsmentioning

confidence: 99%

Effect of Fluoride Varnish and Solution on Enamel Erosion in vitro

Sorvari¹,

et al. 1994

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“…The specimens were finally polished with 5-and 0.3-,um alumina on polishing cloths in order to expose the outer 300 500 pm deep enamel layers, so that the layers would contain low fluoride levels [Weathered et al, 1983;Luoma et al. 1986] and no prismless enamel [Whittaker, 1982;Kodaka et al, 1990Kodaka et al, , 1991, The specimens were cut into about 5x5-m m enamel areas (25 mm2), 2 mm thick and etched with 30% phosphoric acid at pH 1.4 for 60 s after ultrasonic cleaning. Sixteen samples were randomly divided among the 4 subjects with each group of four samples attached to a resin plate with simple clasps for each subject.…”

Section: Preparation O F Specimensmentioning

confidence: 99%

Brushing-lnduced Effects with and without a Non-Fluoride Abrasive Dentifrice on Remineralization of Enamel Surfaces Etched with Phosphoric Acid

Kuroiwa

Kodaka²,

Abe³

1994

Caries Res

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The remineralization of acid-etched enamel after brushing with and without a non-fluoride abrasive dentifrice was investigated using scanning electron microscopy after sodium hypochlorite (NaOCl) or ethylenediamine tetra-acetic acid treatment, of the specimens. Ground enamel pieces were etched with 30% phosphoric acid for 60 s and attached to resin plates. They were exposed to the oral environment of 4 subjects for 8 weeks and brushed for 1 min daily. Paste and brushing caused the acid-etched enamel to erode selectively, i.e., deep grooves were formed in the prism peripheral regions, while projections of the prism bodies showing a relatively low density of crystallites were retained. Brushing without dentifrice, on the other hand, caused about 0.5 μm thick pellicle with tag-like structures to cover the etched enamel. Fine mineral granules, presumably derived from saliva and minute fragments of etched crystallites, were precipitated on the enamel surface as a thin layer with the pellicle. These results indicate that daily brushing without dentifrice induces the remineralization of acid-etched enamel by depositing salivary components, while paste brushing will lead to abrasion of the weakened enamel.

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“…However, the structure of enamel is not homogeneous; the initial layer at the dentino-enamel junction (DEJ), and particularly the final, superficial layer at the enamel surface, is structurally distinct from inner (bulk) enamel. Inner enamel is characterized by the parallel arrangement of long apatite crystals within decussating prisms (layered in alternate directions), while outer enamel appears as a relatively thin region where enamel prisms display a honeycomb pattern in both rodents Risnes et al, 1996] and humans [Kodaka et al, 1991]. Subsequent to this, a very thin layer of prismless final enamel is deposited at the enamel surface within which the enamel crystals generally run perpendicular to the tooth surface.…”

Section: Introductionmentioning

confidence: 99%

Comparative Temporospatial Expression Profiling of Murine Amelotin Protein during Amelogenesis

Somogyi-Ganss

Nakayama

Iwasaki

et al. 2011

Cells Tissues Organs

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Tooth enamel is formed in a typical biomineralization process under the guidance of specific organic components. Amelotin (AMTN) is a recently identified, secreted protein that is transcribed predominantly during the maturation stage of enamel formation, but its protein expression profile throughout amelogenesis has not been described in detail. The main objective of this study was to define the spatiotemporal expression profile of AMTN during tooth development in comparison with other known enamel proteins. A peptide antibody against AMTN was raised in rabbits, affinity purified and used for immunohistochemical analyses on sagittal and transverse paraffin sections of decalcified mouse hemimandibles. The localization of AMTN was compared to that of known enamel proteins amelogenin, ameloblastin, enamelin, odontogenic ameloblast-associated/amyloid in Pindborg tumors and kallikrein 4. Three-dimensional images of AMTN localization in molars at selected ages were reconstructed from serial stained sections, and transmission electron microscopy was used for ultrastructural localization of AMTN. AMTN was detected in ameloblasts of molars in a transient fashion, declining at the time of tooth eruption. Prominent expression in maturation stage ameloblasts of the continuously erupting incisor persisted into adulthood. In contrast, amelogenin, ameloblastin and enamelin were predominantly found during the early secretory stage, while odontogenic ameloblast-associated/amyloid in Pindborg tumors and kallikrein 4 expression in maturation stage ameloblasts paralleled that of AMTN. Secreted AMTN was detected at the interface between ameloblasts and the mineralized enamel. Recombinant AMTN protein did not mediate cell attachment in vitro. These results suggest a primary role for AMTN in the late stages of enamel mineralization.

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Structural and Distribution Patterns of Surface ‘Prismless’ Enamel in Human Permanent Teeth

Cited by 58 publications

References 15 publications

Effect of Fluoride Varnish and Solution on Enamel Erosion in vitro

Effect of Fluoride Varnish and Solution on Enamel Erosion in vitro

Brushing-lnduced Effects with and without a Non-Fluoride Abrasive Dentifrice on Remineralization of Enamel Surfaces Etched with Phosphoric Acid

Comparative Temporospatial Expression Profiling of Murine Amelotin Protein during Amelogenesis

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