Structural and conformational changes of regeneratedAntheraea pernyi silk fibroin films treated with methanol solution

Abstract

ABSTRACT:The regenerated Antheraea pernyi silk fibroin films were prepared from calcium nitrate solution and crystallized with aqueous methanol solution. The structural and conformational changes were investigated by the X-ray diffraction method and infrared spectroscopy upon methanol treatment. The concentration and treatment time of aqueous methanol solution have a great influence on the conformation of regenerated films. The conformational change from a random coil to ␤-sheet structure was occurred within 5 min by the treatment of 40 -60% methanol solution. As the methanol concentration increases up to 100%, the transition time of conformational change is delayed, and the structural change is not occurred in the case of 100% methanol used. The content of a ␣-helix, ␤-sheet, and random coil conformation was calculated and examined to find out the effect of methanol treatment on the conformation changes. The ␤-sheet structure can be transformed from a random coil, while the content of ␣-helix structure is not changed, regardless of methanol treatment.

Enzymatic degradation of Antheraea pernyi silk fibroin 3D scaffolds and fibers

“…In contrast, the natural Ap-SF fiber containing moreˇ-sheet structures has long-term degradation characteristics. It has been reported that the molecular conformation of the Ap-SF materials changes after treatment with methanol solution, ethanol and other organic solvents and changes with temperature [6][7][8][9]. These observations will allow us to adjust the molecular conformation of Ap-SF based biomaterials which would be applied in tissue engineering or tissue inducing, and to control the biodegradation rates, so as to satisfy numerous tissue regeneration requirements.…”

“…The results indicated that the regenerated Ap-SF, like B. mori silk fibroin and collagen, can support hBMSCs attachment, growth and phenotypic maintenance, and has better biocompatibilities for hBMSCs in vitro culture. Based on these features, interest has arisen into the use of Ap-SF as a starting material for advanced biomedical applications such as cell culture substrates, surgical mending materials and tissue engineering scaffolds [5][6][7][8].…”

Gelation behavior of Antheraea pernyi silk fibroin

“…By studying the attachment, morphology, growth and phenotype of human bone marrow derived mesenchymal stem cells (hBMSCs) cultured on Ap-SF films, it was found that Ap-SF, such as B. mori silk fibroin and collagen, could support hBMSC attachment, growth and phenotypic maintenance, and has better biocompatibilities for in vitro culture of hBMSCs [12]. Based on these features, interest has risen in the use of Ap-SF as a starting material for advanced biomedical applications such as cell culture substrates, surgical mending materials and tissue engineering scaffolds [13][14][15][16][17][18][19][20].…”

Effect of ethanol concentration on the infrared spectroscopic characteristics of silk beads

“…최근에 들어 세포 및 조직에 대한 실크단백질의 생체 적합성이 검증되고 있어 이를 활용한 실크단백질을 이용 한 의료용 소재 개발 연구에 관한 관심이 증가하고 있다 (Vepari and Kaplan 2007, Meinel et al 2005, Minoura et al 1995. 고막재생용 실크 패치 (Kim et al 2010), 치과용 실크 차폐막 (Seok et al 2013, Kweon et al 2010a, 조직 공학용 실크 지지체 (Cho et al 2013, Zhao et al 2011, 약물전달용 매트릭스 ) 등 다양한 생체재 료로서 실크 단백질을 활용하기 위한 연구 개발이 추진 되고 있다 (Silva et al 2008, Hu et al 2006, Kweon and Park 1999, Tsukada 1986 …”