Structural and compositional diversity in the kainate receptor family

Selvakumar, Purushotham; Lee, Joon; Khanra, Nandish; He, Changhao; Munguba, Hermany; Kiese, Lisa; Broichhagen, Johannes; Reiner, Andreas; Levitz, Joshua; Meyerson, Joel R.

doi:10.1016/j.celrep.2021.109891

Cited by 24 publications

(25 citation statements)

References 83 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Indeed, with this toolset of impermeable dyes across the visible spectrum, this maneuver enables 3-color imaging of surface proteins, by using SNAP, CLIP and Halo-tags. 32 In addition, we found a potential secondary binding site of SNAP:Sulfo549 by molecular docking, whereby both sulfonates form a hydrogen bond to the protein.…”

Section: Discussionmentioning

confidence: 83%

Sulfonated red and far-red rhodamines to visualize SNAP- and Halo-tagged cell surface proteins

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 83%

Sulfonated red and far-red rhodamines to visualize SNAP- and Halo-tagged cell surface proteins

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…Even more, the possibility to use orthogonal tags allows the use of different colors, rapidly adding to the portfolio of multiplexed experiments to visualize receptor pairs and their ligand interaction in live cells. Recent examples include the determination of SNAP-mGluR4 co-localization (with Ca v 2.1 and Bassoon) by two-color dSTORM microscopy (Siddig et al, 2020), the compositional diversity of differently tagged-kainate receptor assembly by three-color single molecule TIRF microscopy (Selvakumar et al, 2021), and the endocytosis and turnover of Halo-TfR by widefield imaging (Jonker et al, 2020). Alternatively, visualization of cell surface ligand binding, endo- and exocytosis, as well as receptor clustering was achieved using ligands covalently linked to fluorophores (Alborzinia et al, 2013; Ast et al, 2020; Nanda & Lorsch, 2014; Paarmann et al, 2016; Ramachandran et al, 2021; Trujillo et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

A versatile Halo- and SNAP-tagged BMP/TGFβ receptor library for quantification of cell surface ligand binding

Jatzlau¹,

Burdzinski

Trumpp³

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

The TGFβ superfamily of secreted growth factors comprises more than 30 members including TGFβs, BMPs and Activins. While all TGFβ superfamily members signal through heteromeric receptor complexes to regulate a plethora of developmental and homeostatic processes, each ligand possesses a unique affinity towards a subset of BMP and TGFβ type I and type II receptors. Whereas the Activin and TGFβ class display a higher affinity towards type II receptors, BMPs and GDFs preferentially bind to type I receptors. Sofar, the lack of specific antibodies and chemical biology tools hampered simultaneous testing of ligand binding towards all BMP and TGFβ receptors. Here we present a N-terminally Halo- and SNAP-tagged TGFβ/BMP receptor library to visualize the receptor complexes in dual color. In combination with novel fluorescently labeled TGFβ superfamily ligands, we established a Ligand Surface Binding Assay (LSBA) for optical quantification of receptor-dependent growth factor binding for Activin A, TGFβ1 and BMP9 in a cellular context. We confirm ligand-receptor interface specificity by identifying BMPR2- or ALK2-mutants that switch from a low-affinity Activin A- or BMP9-receptor to a high-affinity receptor, respectively.

show abstract

“…The excessive apoptosis in gills reduced the gluk2 −/− mutant’s resistance to cold-temperature exposure. Moreover, the carbohydrates and lipids of fish would be mobilized strategically after the energy homeostasis is disrupted under cold stress [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic and Phenotypic Analysis of CRISPR/Cas9-Mediated gluk2 Knockout in Zebrafish

Yan

Xiao-nan

et al. 2022

Genes

View full text Add to dashboard Cite

As a subtype of kainite receptors (KARs), GluK2 plays a role in the perception of cold in the periphery sensory neuron. However, the molecular mechanism for gluk2 on the cold stress in fish has not been reported. In this article, real-time PCR assays showed that gluk2 was highly expressed in the brain and eyes of adult zebrafish. To study the functions of gluk2, gene knockout was carried out using the CRISPR/Cas9 system. According to RNA-seq analysis, we selected the differentially expressed genes (DEGs) that had significant differences in at least three tissues of the liver, gill, intestine, skin, brain, and eyes. Gene Ontology (GO) enrichment analysis revealed that cry1ba, cry2, per1b, per2, hsp70.1, hsp70.2, hsp70l, hsp90aa1.1, hsp90aa1.2, hspb1, trpv1, slc27a1b, park2, ucp3, and METRNL were significantly enriched in the ‘Response to temperature stimulus’ pathway. Through behavioral phenotyping assay, the gluk2−/− larval mutant displayed obvious deficiency in cold stress. Furthermore, TUNEL (TdT-mediated dUTP Nick-End Labeling) staining proved that the gill apoptosis of gluk2−/− mutant was increased approximately 60 times compared with the wild-type after gradient cooling to 8 °C for 15 h. Overall, our data suggested that gluk2 was necessary for cold tolerance in zebrafish.

show abstract

Structural and compositional diversity in the kainate receptor family

Abstract: Highlights d The cryo-EM structure of the GluK1 homomer d Complex co-expression patterns of two or more KAR subunit types across cell types d KAR di-heteromers can assemble with 3:1, 2:2, and 1:3 stoichiometries d KARs can assemble as tri-heteromers and tetra-heteromers

Cited by 24 publications

References 83 publications

Sulfonated red and far-red rhodamines to visualize SNAP- and Halo-tagged cell surface proteins

Sulfonated red and far-red rhodamines to visualize SNAP- and Halo-tagged cell surface proteins

A versatile Halo- and SNAP-tagged BMP/TGFβ receptor library for quantification of cell surface ligand binding

Transcriptomic and Phenotypic Analysis of CRISPR/Cas9-Mediated gluk2 Knockout in Zebrafish

Contact Info

Product

Resources

About