Structural and Biological Properties of the Drosophila Insulin-like Peptide 5 Show Evolutionary Conservation

Abstract

We report the crystal structure of two variants of Drosophila melanogaster insulin-like peptide 5 (DILP5) at a resolution of 1.85 Å . DILP5 shares the basic fold of the insulin peptide family (T conformation) but with a disordered B-chain C terminus. DILP5 dimerizes in the crystal and in solution. The dimer interface is not similar to that observed in vertebrates, i.e. through an anti-parallel ␤-sheet involving the B-chain C termini but, in contrast, is formed through an anti-parallel ␤-sheet involving the B-chain N termini. DILP5 binds to and activates the human insulin receptor and lowers blood glucose in rats. It also lowers trehalose levels in Drosophila. Reciprocally, human insulin binds to the Drosophila insulin receptor and induces negative cooperativity as in the human receptor. DILP5 also binds to insect insulin-binding proteins. These results show high evolutionary conservation of the insulin receptor binding properties despite divergent insulin dimerization mechanisms.The ligands and receptors of the insulin peptide family constitute an ancient metazoan signaling system that plays a crucial pleiotropic role in cell growth, metabolism, reproduction, and longevity (1-7).The mammalian insulin receptor belongs to the family of receptor-tyrosine kinases and is composed of two ␣ subunits and two ␤ subunits linked together by disulfide bonds (for review, see Refs. 4 and 8 -10). The existence of a homologue of the mammalian insulin receptor in Drosophila melanogaster (DIR) 2 was suggested in 1985 by Petruzzelli et al. (11), who identified a glycoprotein of 350 -400 kDa that binds bovine insulin specifically with moderate affinity (15 nM). The cDNA sequence of the DIR is remarkably similar to that of the mammalian insulin and IGF-I receptors (with 33% sequence identity) except for substantial N-and C-terminal extensions (12, 13).In evolution, there is a single receptor from Cnidarians up to and including Amphioxus (Branchiostoma californiense), the phylum closest to vertebrates (for review see Refs. 1, 4 -6). In vertebrates, gene duplications resulted in three related receptors; that is, the insulin receptor, the type I IGF receptor, and the orphan insulin receptor-related receptor (1, 5).In humans, members of the insulin peptide family include insulin, the insulin-like growth factors I and II, and seven relaxin-related peptides (for review, see Ref. 14). The same basic fold is shared for all molecules in the superfamily whose structure is known; the B domain contains a single ␣-helix that lies across the two ␣-helices of the A domain (15) and two canonical disulfide bridges that connect the A-and Bchains, whereas an intrachain disulfide bridge is present in the A-chain.The D. melanogaster genome contains seven insulin-like genes that are expressed in a highly tissue-and stage-specific patterns, dilp1-7 (16). dilp2 is the most related to human insulin with 35% sequence identity, whereas dilp5 has 27.8% identity (16).So far, the structures of only two invertebrate insulin-like peptides have been determined by N...

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