Structural and Biological Characteristics of Self-Organising Chitosan Hydrogels

Abstract

Creating innovative methods of treatment and regeneration of damaged tissues or organs is a key challenge of the twenty-first century. The aim of this study was to determine the possibility of producing and characterising the properties of self-organising chitosan hydrogels prepared with the use of chitosan lactate/chloride and disodium hydrogen phosphate dodecahydrate as a cross-linking agent. The structure and supramolecular architecture of the biomaterials were evaluated by Fourier-transform infrared spectroscopy and polarised optical microscopy. Biological studies assessed cytotoxicity by contact with a human colon adenocarcinoma cell line. The colourimetric resazurin assay showed that the obtained chitosan hydrogels are non-cytotoxic materials. Thus, self-organising biomaterialshold great promise for application in tissue engineering.

Nanoarchitectonics and Biological Properties of Nanocomposite Thermosensitive Chitosan Hydrogels Obtained with the Use of Uridine 5′-Monophosphate Disodium Salt

“…The assessment of the genotoxicity of the biomaterials was based on the alkaline version of the comet assay. The methods of carrying out the determinations were identical to the procedures presented in our articles [ 27 , 33 ].…”