Structural and antioxidative properties of royal jelly protein by partial enzymatic hydrolysis

Li, Shanshan; Tao, Lingchen; Peng, Shiqin; Yu, Xinyu; Ma, Xiaobin; Hu, Fuliang

doi:10.1016/j.fshw.2023.02.046

Cited by 5 publications

(1 citation statement)

References 51 publications

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“…In fact, the absorption of highmolecular-weight proteins by cells presents a significant challenge, as proteins in royal jelly struggle to penetrate the cell membrane to exert their effects. Enzymatic hydrolysis can reduce the molecular weight of royal jelly proteins and improve the bioactivity [40]. Thus, in this study, we utilized pancreatic trypsin hydrolysis to prepare low-molecularweight RJP, and revealed its excellent antioxidant and anti-inflammatory properties in HDFs.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant and Anti-Inflammatory Properties of Hydrolyzed Royal Jelly Peptide in Human Dermal Fibroblasts: Implications for Skin Health and Care Applications

Yan,

Zhu,

Guan

et al. 2024

Bioengineering

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Hydrolyzed royal jelly peptide (RJP) has garnered attention for its health-promoting functions. However, the potential applications of RJP in skincare have not been fully explored. In this study, we prepared RJP through the enzymatic hydrolysis of royal jelly protein with trypsin and investigated its antioxidant and anti-inflammatory properties on primary human dermal fibroblasts (HDFs). Our results demonstrate that RJP effectively inhibits oxidative damage induced by H2O2 and lipid peroxidation triggered by AAPH and t-BuOOH in HDFs. This effect may be attributed to the ability of RJP to enhance the level of glutathione and the activities of catalase and glutathione peroxidase 4, as well as its excellent iron chelating capacity. Furthermore, RJP modulates the NLRP3 inflammasome-mediated inflammatory response in HDFs, suppressing the mRNA expressions of NLRP3 and IL-1β in the primer stage induced by LPS and the release of mature IL-1β induced by ATP, monosodium urate, or nigericin in the activation stage. RJP also represses the expressions of COX2 and iNOS induced by LPS. Finally, we reveal that RJP exhibits superior antioxidant and anti-inflammatory properties over unhydrolyzed royal jelly protein. These findings suggest that RJP exerts protective effects on skin cells through antioxidative and anti-inflammatory mechanisms, indicating its promise for potential therapeutic avenues for managing oxidative stress and inflammation-related skin disorders.

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Section: Discussionmentioning

confidence: 99%