“…2A where domain I, domain II, the crossover loop, the HxxxD motif, and the DFGWG motif are highlighted. Previously, several groups have constructed protein models for AAT enzymes from melon ( Cucumis melo ), mountain papaya fruit ( Vasconcellea pubescens ), and strawberry ( Fragaria x ananassa ) and paired in silico and in vitro analysis to characterise the mechanism of catalysis for these enzymes, as well as the impact of AAT structure on substrate specificity (Morales-Quintana et al, 2011, 2013; Galaz et al, 2013; Navarro-Retamal et al, 2016). From this work, it has been determined that both the alcohol and acyl-CoA substrates access the catalytic His and Asp residues of the HxxxD motif through a solvent channel that runs through the centre of the protein between domains I and II.…”