Structural analysis of water-soluble polysaccharides isolated from Panax notoginseng

Chan, Mi Kaythi; Yu, Yang; Wulamu, S.; Wang, Yuan; QiDi, Wang; Zhou, Yifa; Sun, Lin

doi:10.1016/j.ijbiomac.2020.03.233

Cited by 37 publications

(27 citation statements)

References 32 publications

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“…To obtain polysaccharide fractions from the various Panax species, different extraction methods are used to extract different polysaccharides, including hot water/ethanol extraction [ 29 , 34 , 35 , 36 ], alkaline extraction [ 37 , 38 ], enzymatic extraction [ 39 , 40 , 41 ], and ethylenediaminetetraacetic acid (EDTA) extraction [ 42 ]. Other new methods, such as ultrasonic extraction [ 43 , 44 ] and microwave extraction [ 45 ], have been performed to extract polysaccharides from Panax species and these can improve extraction efficiency and decrease extraction time.…”

Section: Extraction and Purification Methodsmentioning

confidence: 99%

“…Most studies report that hot water extraction was used to isolate the polysaccharides from three Panax species, which is the most classical and convenient method for isolating water-soluble crude polysaccharide [ 29 , 46 ]. Briefly, the roots of Panax species were soaked in water overnight and boiled in water (2–4 times, 2–6 h each time) to obtain the supernatants, which were centrifuged at 5000 rpm for 30 min and concentrated under vacuum.…”

Section: Extraction and Purification Methodsmentioning

confidence: 99%

“…A new study to isolate water-soluble polysaccharides from notoginseng discovered a starch-like polysaccharide, PNPN, and its pectin fractions. Its structural characteristics were analyzed using multiple methods and it contains Gal, Ara, GalA, and Rha, with different sugar compositions and molecular weights [ 29 ]. Among these sub-fractions, two fractions mainly include 1,4-β- d -galactans, 1,5-α- l -arabinan, and AG-II and three fractions consist of HG, together with different ratios of RG-I and RG-II domains [ 28 ].…”

Section: Structural Characteristicsmentioning

confidence: 99%

“…Currently, most studies focus on the purification, structural analysis, and bioactivities of polysaccharides from the three above-mentioned different Panax species [ 29 , 30 , 31 ]. Ginseng polysaccharides have been deeply studied and recent findings have been extensively summarized [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

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Comparisons of Isolation Methods, Structural Features, and Bioactivities of the Polysaccharides from Three Common Panax Species: A Review of Recent Progress

Zhang

Liu

et al. 2021

Molecules

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Panax spp. (Araliaceae family) are widely used medicinal plants and they mainly include Panax ginseng C.A. Meyer, Panax quinquefolium L. (American ginseng), and Panax notoginseng (notoginseng). Polysaccharides are the main active ingredients in these plants and have demonstrated diverse pharmacological functions, but comparisons of isolation methods, structural features, and bioactivities of these polysaccharides have not yet been reported. This review summarizes recent advances associated with 112 polysaccharides from ginseng, 25 polysaccharides from American ginseng, and 36 polysaccharides from notoginseng and it compares the differences in extraction, purification, structural features, and bioactivities. Most studies focus on ginseng polysaccharides and comparisons are typically made with the polysaccharides from American ginseng and notoginseng. For the extraction, purification, and structural analysis, the processes are similar for the polysaccharides from the three Panax species. Previous studies determined that 55 polysaccharides from ginseng, 18 polysaccharides from American ginseng, and 9 polysaccharides from notoginseng exhibited anti-tumor activity, immunoregulatory effects, anti-oxidant activity, and other pharmacological functions, which are mediated by multiple signaling pathways, including mitogen-activated protein kinase, nuclear factor kappa B, or redox balance pathways. This review can provide new insights into the similarities and differences among the polysaccharides from the three Panax species, which can facilitate and guide further studies to explore the medicinal properties of the Araliaceae family used in traditional Chinese medicine.

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Section: Extraction and Purification Methodsmentioning

confidence: 99%

Section: Extraction and Purification Methodsmentioning

confidence: 99%

Section: Structural Characteristicsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Comparisons of Isolation Methods, Structural Features, and Bioactivities of the Polysaccharides from Three Common Panax Species: A Review of Recent Progress

Zhang

Liu

et al. 2021

Molecules

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“…The signals at H-1/C-1 104.37/4.63 and 100.32/4.95 ppm were assigned to !4)-b-Galp-(1! and a-Galp-(1!, respectively (24,25). Similarly, the signals at H-1/ C-1 92.22/5.28, 96.27/4.60 and 99.50/5.32 ppm were assigned to !4)-a-Glcp, b-D-Glcp-(1!…”

Section: Nmr Spectroscopy Analysismentioning

confidence: 98%

Extraction, Structural Characterization, and Anti-Hepatocellular Carcinoma Activity of Polysaccharides From Panax ginseng Meyer

et al. 2021

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Polysaccharides are the main active ingredients of ginseng. To extract the most effective polysaccharides against hepatocellular carcinoma (HCC), we isolated and characterized the polysaccharides from the mountain cultivated ginseng (MCG) and compared their composition and cytotoxic effect with cultivated ginseng (CG) polysaccharide against HepG2 cell lines for the first time. MCG polysaccharides and CG polysaccharides were fractionated into two fractions such as MTPS-1, MTPS-2 and CTPS-1, CTPS-2 by salting out, respectively. Compared to CG, MCG possessed appreciable cytotoxic effect against HepG2 cells among that MTPS-1 possess fortified effect. Then, MTPS-1 was selected for further isolation process and seven acidic polysaccharides (MCGP-1–MCGP-7) were obtained using ethanol precipitation, ion-exchange, and gel permeation chromatography techniques. Structural characteristics of the polysaccharides (MCGP-1–MCGP-7) were done by adapting methylation/GC-MS and NMR analysis. Overall, MCGP-3 polysaccharide was found to possess significant cytotoxic effect against HepG2 cells with the IC50 value.

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Structural Characterization of Polysaccharides Isolated from Panax notoginseng Medicinal Residue and Its Protective Effect on Myelosuppression Induced by Cyclophosphamide

Liu

et al. 2021

Chemistry & Biodiversity

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This study aims to establish the isolation and purification method of polysaccharides from medicinal residue of Panax notoginseng (PPN). The structure and protective effect of PPN on myelosuppression mice were investigated. One neutral polysaccharide (NPPN) and five acidic polysaccharides (APPN I, APPN II-A, APPN II-B, APPN III-A, and APPN III-B) were obtained. The results confirmed that NPPN, APPN I and APPN II-A are glycan with 1, 4 main chains. APPN III-A is a glycan. APPN II-B and APPN III-B are homogalacturonan pectin with 1, 4 main chains. This study demonstrated that NPPN played a bone marrow protective role in myelosuppression mice induced by cyclophosphamide. NPPN could relieve cell cycle arrest, reduce the apoptosis rate of marrow cells, and improve granulocyte-macrophage colony-stimulating (GM-CSF), thermoplastic polyolefin (TPO) and erythropoietin (EPO) serum level, which contributes to promoting the proliferation of hematopoietic cells.

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Structural analysis of water-soluble polysaccharides isolated from Panax notoginseng

Cited by 37 publications

References 32 publications

Comparisons of Isolation Methods, Structural Features, and Bioactivities of the Polysaccharides from Three Common Panax Species: A Review of Recent Progress

Comparisons of Isolation Methods, Structural Features, and Bioactivities of the Polysaccharides from Three Common Panax Species: A Review of Recent Progress

Extraction, Structural Characterization, and Anti-Hepatocellular Carcinoma Activity of Polysaccharides From Panax ginseng Meyer

Structural Characterization of Polysaccharides Isolated from Panax notoginseng Medicinal Residue and Its Protective Effect on Myelosuppression Induced by Cyclophosphamide

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