Structural analysis of the O-polysaccharide from the lipopolysaccharide of Azospirillum brasilense S17

Monosaccharide composition of the outer membrane lipopolysaccharide and O-chain from the freshwater cyanobacterium Microcystis aeruginosa NIES-87

“…Mild hydrolysis followed by centrifugation typically yields O‐chain and R‐core in the supernatant (Fedonenko et al . ; Wang et al . ).…”

“…The LPS fraction (10 mg ml −1 ) was dissolved in 1% acetic acid and mildly hydrolysed at 100°C for 2·5 h (Fedonenko et al . ; Wang et al . ).…”

Structural elucidation of the capsular polysaccharide isolated from Kaistella flava

“…The structure of this polysaccharide and the kind of monosaccharides employed in its construction confers to the bacterial membranes a rather neutral if not lipophilic character, a feature shared from the capsule or the lipopolysaccharides of many other bacteria living in the same habitat, like most of the members of the Rhizobiaceae family, 9 or other genera as Azospirillum, 10 Xanthomonas or the phytopathogenic Pseudomonas and Burkholderia species. 11 The role of this polysaccharide in the environmental adaptation process of this bacterium is still unknown, and further work is needed to address this point.…”

Revised structure of the repeating unit of the O-specific polysaccharide from Azospirillum lipoferum strain SpBr17

“…1 OAc β-D-Glcp ⎜ These results have been cited repeatedly by researchers working on Azospirillum surface polysaccharides. [5][6][7][8]10 Recently, we have published the structure of A. lipoferum SpBr17 lipid A. 11 During preparation of the lipid A moiety, a large amount of the O-specific polysaccharide was obtained.…”

“…[1][2][3] Carbohydrate polymers such as EPS, CPS, and LPS play an important role in the interactions between plant and bacteria. 1 Studies concerning the structural characteristics of Azospirillum lipopolysaccharides, the main constituents of the outer leaflet of the outer membrane of Gram-negative bacteria, have been performed only for six strains: A. lipoferum SpBr17, 4 Sp59b, 5 and SR65, 6 A. brasilense Sp245 7 and S17, 8 as well as A. irakense KBC1. 9 The structure of the O-specific polysaccharide from A. lipoferum SpBr17 was presented at and published in the Abstracts of the International Congress on Nitrogen Fixation in Cancun (Mexico, 1992).…”