Structural analysis of the<i>Drosophila</i>rpA1 gene, a member of the eucaryotic ‘A’ type ribosomal protein family

Qian, Su Wen; Zhang, Jing Yu; Kay, Mark A.; Jacobs-­Lorena, Marcelo

doi:10.1093/nar/15.3.987

Cited by 74 publications

(43 citation statements)

References 31 publications

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“…32 P]dCTP-labeled DNA probe specific for the Drosophila ribosomal protein A1 (33 (12,21). In the course of one of these searches, it became evident that the sequence of the recently cloned gene for succinate dehydrogenase, subunit b (SDHb) of Drosophila melanogaster (24) contains a typical wild-type IRE apparently close to the 5Ј-end of the predicted mRNA (Fig.…”

Section: Methodsmentioning

confidence: 99%

Succinate Dehydrogenase b mRNA of Drosophila melanogaster Has a Functional Iron-responsive Element in Its 5′-Untranslated Region

Kohler¹,

Henderson²,

Kühn³

1995

Journal of Biological Chemistry

126

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Section: Methodsmentioning

confidence: 99%

Succinate Dehydrogenase b mRNA of Drosophila melanogaster Has a Functional Iron-responsive Element in Its 5′-Untranslated Region

Kohler¹,

Henderson²,

Kühn³

1995

Journal of Biological Chemistry

126

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“…This fragment contains the 5Ј one-third of the ftz transcribed sequences and 6.1 kb of upstream sequences, which are necessary for correct expression of the ftz gene (17). A 1.3-kb SalI-BamHI genomic fragment of the ribosomal protein gene rpA1 (28), which includes the 3Ј two-thirds of the rpA1 transcribed sequences, was then introduced into this vector, resulting in the f5r3 hybrid gene (see Fig. 2A).…”

Section: Construction Of Transgenes (I) F5r3 and R5f3mentioning

confidence: 99%

Determinants of Drosophila fushi tarazu mRNA Instability

Riedl

Jacobs-Lorena

1996

Molecular and Cellular Biology

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The fushi tarazu gene is essential for the establishment of the Drosophila embryonic body plan. When first expressed, in early embryogenesis, fushi tarazu mRNA is uniformly distributed over most of the embryo. Subsequently, fushi tarazu mRNA expression rapidly evolves into a pattern of seven stripes that encircle the embryo. The instability of fushi tarazu mRNA is probably crucial for attaining this localized pattern of expression. mRNA stability in transgenic embryos was measured by a new method that does not use drugs or external interference. Experiments using hybrid genes that fuse fushi tarazu sequences to those of the stable ribosomal protein A1 mRNA provide evidence for at least two destabilizing elements in the fushi tarazu mRNA, one located within the 5 one-third of the mRNA and the other near the 3 end (termed FIE3 for ftz instability element 3). The FIE3 lies within a 201-nucleotide sequence just upstream of the polyadenylation signal and can act autonomously to destabilize a heterologous mRNA. Further deletion constructs identified an essential 68-nucleotide element within the FIE3. Lack of homology between this element and other previously identified destabilization sequences suggests that FIE3 contains a novel RNA destabilization element.mRNA stability plays a key role in regulating the expression of many transiently expressed genes, such as cytokines, oncogenes, and developmentally important genes (36). While much has been learned about the regulation of mRNA stability in cultured cells (4), few studies have been conducted with whole organisms or in a developmental context (36). This is due in part to the lack of a convenient method to assay mRNA stability in whole organisms. In this paper, we present a new method to measure mRNA stability in Drosophila embryos.We have directed our attention to genes which establish the Drosophila embryonic body plan. Early zygotic mRNAs belonging to the gap, pair-rule, and segment polarity group of genes are expressed only transiently in the developing Drosophila embryo. As such, these mRNAs are likely to be unstable, and as indicated below, instability is probably crucial for achieving correct spatial and temporal pattern of expression. Here we focus on fushi tarazu (ftz), one of the best-characterized Drosophila pair-rule genes. The ftz mRNA is among the shortest-lived mRNAs (6-to 14-min half-life) among higher eukaryotes (4, 9) and shares the cytoplasm with a majority of mostly stable mRNAs.ftz transcripts are first expressed at low levels at embryonic nuclear cycle 11 (about 1 h 40 min after egg laying at 25ЊC) in a broad band between 15 and 65% egg length (measured from the posterior pole) (5, 11, 13). During cellularization of the blastoderm (2 h 10 min to 2 h 50 min), ftz mRNA becomes transiently restricted to four broad bands and finally to seven narrow stripes that encircle the embryo (5, 13, 38, 45). Thus, it is during this very short time that the spatial pattern of ftz mRNA expression is established. Formation of stripes probably occurs by the cessati...

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“…This phenomenon has been demonstrated for transcripts from both universal housekeeping genes such as the ribosomal protein genes (33) and from key regulatory genes such as bicoid (34). It is possible, therefore, that ME31B represents a translation initiation factor related to eIF4A but with a more specialized role.…”

mentioning

confidence: 92%

A second maternally expressed Drosophila gene encodes a putative RNA helicase of the "DEAD box" family.

Valoir

Tucker

Belikoff

et al. 1991

Proc. Natl. Acad. Sci. U.S.A.

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Recently, a family of proteins containing the conserved motif Asp-Glu-Ala-Asp, the "DEAD box" proteins, has been identified. This family is typified by the eukaryotic translation initiation factor eIF4A, and its members are believed to share the functional property of ATP-dependent RNA unwinding. One of the previously identified members of this family (vasa) is the product of a maternally expressed gene from DrosophUa melanogaster that is known to play a role in the formation of the embryonic body plan. We report here the isolation of a Drosophila gene that has an mRNA expression pattern somewhat similar to that of vasa and also encodes a DEAD box protein. We have termed this gene ME3JB to reflect its maternal (ovarian germ-line) expression and its location within the 31B chromosome region. Comparisons with the other members of this family reveal that although ME31B is most like the protein Tifl/Tif2, which probably represents the Saccharomyces cerevisiae version of eIF4A, it is unlikely that ME31B represents the Drosophila eIF4A protein per se. A search for mutations in the ME3JB gene has established that the P element which causes the female-sterile mutation flipper lies in the 3' flank of the ME3JB gene.Recently a family of proteins that function as ATP-dependent RNA helicases has been identified (1).These proteins share a domain of about 370 amino acids in which a series of highly conserved motifs are arranged in identical order and with very similar spacing. Two of these conserved sequences represent specialized versions of the A and B motifs previously recognized in other ATP-binding proteins (2,3). The four-amino-acid sequence Asp-Glu-Ala-Asp (DEAD) is part ofthe specialized version ofthe B motif. These "DEAD box" proteins derive from organisms across all evolutionary orders (Escherichia coli to mouse), and for some of them, genetic and molecular studies have provided indications as to their functions in vivo (4-7). Thus this family of proteins appears to be involved in a wide range of intracellular events in which alteration of RNA secondary structure must play a critical role. These include formation of the initiation complex for mRNA translation, mRNA splicing, and ribosome biosynthesis. Functionally, the eukaryotic translation initiation factor eIF4A, which is the archetypal member of the family, is the one best understood at the molecular level (8-10).

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Structural analysis of theDrosophilarpA1 gene, a member of the eucaryotic ‘A’ type ribosomal protein family

Cited by 74 publications

References 31 publications

Succinate Dehydrogenase b mRNA of Drosophila melanogaster Has a Functional Iron-responsive Element in Its 5′-Untranslated Region

Succinate Dehydrogenase b mRNA of Drosophila melanogaster Has a Functional Iron-responsive Element in Its 5′-Untranslated Region

Determinants of Drosophila fushi tarazu mRNA Instability

A second maternally expressed Drosophila gene encodes a putative RNA helicase of the "DEAD box" family.

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