Structural analysis of Panax ginseng glycoproteins and its anti-oligoasthenozoospermia effect in vivo

Shan, Mengyao; Zhang, Xiaoying; Fang, Xiaoxue; Tian, Jing; Song, Lianlian; Chen, Yinghong; Qiu, Zhidong; Zhu, Difu; Luo, Haoming; Wang, Zhe

doi:10.1016/j.ijbiomac.2021.10.136

Cited by 10 publications

(4 citation statements)

References 29 publications

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“…This effect is attributed to Panax ginsenosides' antioxidant properties and support for the synthesis of nitric oxide (NO)in the neurotelium and endothelial vessels, where Panax ginsenosides act as biochemical donors or precursors. Simultaneously, this has a positive effect on sexual and reproductive function (Ganjkhaniet al 2019;Kuet al 2020;Li et al 2021;Shan et al 2021). Nevertheless, not all of these effects have strong clinical study support.…”

Section: Resultsmentioning

confidence: 99%

Modern possibilities of phytotherapy for male infertility from the standpoint of evidence-based medicine

Bratchikov,

Tyuzikov,

Grekov

2023

RRP

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Introduction: Male infertility is an topical medical and social problem of modern reproductive medicine. Its pharmacotherapy is often empirical in nature, and the most popular method remains the use of various herbal substances (phytotherapy), the effectiveness of which remains still understudied in the framework of evidence-based medicine. Materials and Methods: The results of research, thematic, systematic and Cochrane reviews and meta-analyses were searched in Medline/PubMed medical databases over the past 5 years using the search queries "plants male infertility", "plants sperm", "phytotherapy male infertility", "phytomedicinal therapeutics male infertility", "systematic review", "meta-analysis", and "review". Results: The vast majority of herbal substances offered for the treatment of male infertility demonstrate insufficient or contradictory evidence base for their clinical effectiveness, although some of them can be very useful pharmacotherapeutic options in the combined therapy of male infertility. Conclusion: Not all plant substances with a "reproductive effect" positioned in them actually have proven reproductive effects in studies in humans, therefore, the choice of phytotherapeutic agents in the treatment of idiopathic male infertility should be currently approached extremely carefully, especially in cases when we choose phytotherapy as an option for empirical monotherapy of male reproductive disorders.

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Section: Resultsmentioning

confidence: 99%

Modern possibilities of phytotherapy for male infertility from the standpoint of evidence-based medicine

Bratchikov,

Tyuzikov,

Grekov

2023

RRP

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“…Using the network pharmacology technique, Chen et al discovered that the AKT pathway has a substantial role in oligoasthenozoospermia and was associated with the Yishen Tongluo formula used to treat the condition ( Dong et al, 2022 ). Panax ginseng glycoproteins regulate AKT signaling and promote sex hormones, sperm quality, and the differentiation process in a murine model of oligoasthenozoospermia ( Shan et al, 2021 ). The study performed by Song and his colleagues suggested that the eNOS gene variants T-786C and 4a4b loci are risk factors for idiopathic asthenozoospermia in both Asian and Caucasian populations ( Song et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Yangjing capsule improves oligoasthenozoospermia by promoting nitric oxide production through PLCγ1/AKT/eNOS pathway

et al. 2023

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Background: Oligoasthenozoospermia is an important factor leading to male infertility. Yangjing capsule (YC), a traditional Chinese preparation, displays beneficial effects on male infertility. However, whether YC could improve oligoasthenozoospermia remains unclear.Methods: In this study, we aimed to explore the effect of YC in the treatment of oligoasthenozoospermia. Male Sprague-Dawley (SD) rats were treated with 800 mg/kg ornidazole once daily for 30 days to induce in vivo oligoasthenozoospermia; primary Sertoli cells were treated with 400 μg/mL ornidazole for 24 h to induce in vitro oligoasthenozoospermia.Results: We found that YC improved the testicle and epididymis weight, sperm concentration, sperm progressive motility, serum testosterone, fertility rate and testis morphology in ornidazole-exposed rats and enhanced cell survival in ornidazole-stimulated primary Sertoli cells. YC also inhibited the ornidazole-caused decrease in nitric oxide (NO) generation and the phosphorylation of phospholipase C γ1 (PLCγ1), AKT, and eNOS in vivo and in vitro in oligoasthenozoospermia. Furthermore, the knockdown of PLCγ1 blunted the beneficial effects of YC in vitro.Conclusion: Collectively, our data suggested that YC protected against oligoasthenozoospermia by promoting NO levels through the PLCγ1/AKT/eNOS pathway.

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“…Refer to the previous extraction method and improve it (Fang et al, 2020;Shan et al, 2021). Clean the ginseng root, and separate the ginseng skin and ginseng core by the natural layer ring of the ginseng root.…”

Section: Extraction Of Ginseng Glycoproteinsmentioning

confidence: 99%

“…It has also been suggested that a new role for the ginsenoside RG3 in antiaging via mitochondria function in ultraviolet-irradiated human dermal fibroblasts (Lee et al, 2021). And there are few studies on ginseng glycoproteins, which are mainly studied for their role in improving memory function, treating Alzheimer's disease, protecting the heart, and resisting oligospermia [Luo et al, 2018a;Luo et al, 2018b;Shan et al, 2021(Chen et al, 2021]. The mechanism of action of ginsenosides and ginseng glycoprotein on UV-irradiation-induced skin damage in diabetic rats is even less clear.…”

Section: Introductionmentioning

confidence: 99%

Ginseng glycoprotein and ginsenoside facilitate anti UV damage effects in diabetic rats

Huo²,

He³

et al. 2022

Front. Pharmacol.

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Diabetes mellitus combined with ultraviolet (UV) radiation damage not only brings great mental stress to patients, but also seriously impairs their quality of life. A UV-irradiated diabetic rat trauma skin model was established by us to investigate the effects and possible mechanisms of ginsenoside and glycoprotein on skin trauma repair in UV-irradiated diabetic rats. In the study, ginsenosides and ginseng glycoproteins were extracted from different parts of ginseng roots. It found that it’s easier to prepare saponins in ginseng bark and proteins in ginseng core in large quantities. Since glycoprotein-like metabolites are relatively novel ginseng extracts, specifically characterized its structures. It was verified that the ginseng glycoproteins are not toxic to HaCaT cells and can significantly increase the survival of HaCaT cells after UV damage at the in vitro cellular level. Experiments in vivo were conducted to evaluate the therapeutic effects of ginsenoside and ginseng glycoprotein in a rat model of diabetes mellitus combined with UV irradiation injury. Histopathological changes on rat skin after treatment with ginsenoside and ginseng glycoprotein were evaluated by hematoxylin and eosin (H&E) staining and aldehyde fuchsine staining. The expression levels of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), matrix metalloproteinases (MMPs), hydroxyproline (HYP), interleukin-6 (IL-6), interleukin-1β (IL-1β), and tumor necrosis factor-α (TNF-α) were measured. The results indicate that both ginsenoside and ginseng glycoprotein could improve skin damage and ulcers caused by diabetes combined with UV irradiation and could alleviate a range of skin damage caused by the combination of diabetes and UV irradiation, including peroxidation and collagen fiber loss. Ginsenoside and ginseng glycoproteins can be considered as natural product candidates for the development of new drugs to treat diabetes combined with UV irradiation-induced skin damage.

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Structural analysis of Panax ginseng glycoproteins and its anti-oligoasthenozoospermia effect in vivo

Cited by 10 publications

References 29 publications

Modern possibilities of phytotherapy for male infertility from the standpoint of evidence-based medicine

Modern possibilities of phytotherapy for male infertility from the standpoint of evidence-based medicine

Yangjing capsule improves oligoasthenozoospermia by promoting nitric oxide production through PLCγ1/AKT/eNOS pathway

Ginseng glycoprotein and ginsenoside facilitate anti UV damage effects in diabetic rats

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