Structural analysis of “flexible” liposome formulations: new insights into the skin-penetrating ability of soft nanostructures

Ogunsola, Oluwatosin; Kraeling, Margaret E. K.; Zhong, Sheng; Pochan, Darrin J.; Bronaugh, Robert L.; Raghavan, Srinivasa R.

doi:10.1039/c2sm26614h

Cited by 50 publications

(34 citation statements)

References 33 publications

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“…35,36 For self-assembling organic species in solution, SANS is particularly useful because of the afforded high contrast between a 1 H-rich material and a 2 D-rich solvent. Consequently, one of the more common uses of SANS is for the characterisation of aggregate structures of surfactants, [37][38][39][40][41] 33,52,53 are also investigated, for a variety of purposes including mustard gas decontamination, 50 art restoration, 51 and drug delivery. 33 In this case, selective deuteration of the different components is frequently used to obtain a more detailed structural picture, as highlighted in Fig.…”

Section: Amphiphilic Self-assemblymentioning

confidence: 99%

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Practical applications of small-angle neutron scattering

Hollamby

2013

Phys. Chem. Chem. Phys.

120

116

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Recent improvements in beam-line accessibility and technology have led to small-angle neutron scattering (SANS) becoming more frequently applied to materials problems. SANS has been used to study the assembly, dispersion, alignment and mixing of nanoscale condensed matter, as well as to characterise the internal structure of organic thin films, porous structures and inclusions within steel.Using time-resolved SANS, growth mechanisms in materials systems and soft matter phase transitions can also be explored. This review is intended for newcomers to SANS as well as experts. Therefore, the basic knowledge required for its use is first summarised. After this introduction, various examples are given of the types of soft and hard matter that have been studied by SANS. The information that can be extracted from the data is highlighted, alongside the methods used to obtain it. In addition to presenting the findings, explanations are provided on how the SANS measurements were optimised, such as the use of contrast variation to highlight specific parts of a structure. Emphasis is placed on the use of complementary techniques to improve data quality (e.g. using other scattering methods) and the accuracy of data analysis (e.g. using microscopy to separately determine shape and size). This is done with a view to providing guidance on how best to design and analyse future SANS measurements on materials not listed below.

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Section: Amphiphilic Self-assemblymentioning

confidence: 99%

“…3, the shapes and dimensions of simple aggregate structures that form in solution can be easily elucidated by inspection of the IFT probability distribution. 33 However, for more complicated systems or those containing significant S(Q), the generation and interpretation of the probability distributions is not straightforward. In these cases, assumptions (e.g.…”

mentioning

confidence: 99%

Practical applications of small-angle neutron scattering

Hollamby

2013

Phys. Chem. Chem. Phys.

120

116

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“…In a study by Ogunsola et al. ( 2012 ), the plots in small-angle neutron scattering indicated a direct correlation between the increasing concentrations of Tween and creation of micellar conformations in flexible transfersomes. In the current study, the T 5 formula contained 27.27% Tween and tended to conform into the liposomes ( Figure 3 ).…”

Section: Discussionmentioning

confidence: 94%

Curcumin-loaded nanoliposomes linked to homing peptides for integrin targeting and neuropilin-1-mediated internalization

Kangarlou

Ramezanpour

Balalaie

et al. 2016

Pharmaceutical Biology

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Context: Curcumin, a naturally occurring polyphenol, has been extensively studied for its broad-spectrum anticancer effects. The potential benefits are, however, limited due to its poor water solubility and rapid degradation which result in low bioavailability on administration.Objectives: This study encapsulates curcumin in nanoliposomes including an integrin-homing peptide combined with a C end R neuropilin-1 targeting motif for targeted delivery and receptor-mediated internalization, respectively.Materials and methods: The linear GHHNGR (Glycine–Histidine–Histidine–Asparagine–Glycine–Arginine) was synthesized through F-moc chemistry on 2-chlorotrityl chloride resin and conjugated to oleic acid. The lipoyl-peptide units were then co-assembled with lecithin and 0–75 mole % Tween-80 into liposomes. Curcumin was passively entrapped using a film hydration technique and its degradation profile was examined within seven consecutive days. The cytotoxic effects of the curcumin-loaded liposomes were studied on MCF-7 and MDA-MB-468, during 24 h exposure in MTT assay.Results: The maximum curcumin entrapment (15.5% W/W) and minimum degradation (< 23%) were obtained in a pH switch loading method from 5.7 to 8, in nanoliposomes (< 50 nm) containing oleyl-peptide, lecithin and Tween-80 (1:1:0.75 mole ratio). The oleyl-peptide did not prove any haemolytic activity (< 1.5%) up to 10-fold of its experimental concentration. The curcumin-loaded liposomes displayed significant reduction in the viabilities of MCF-7 (IC50 3.8 μM) and MDA-MB-468 (IC50 5.4 μM).Discussion and conclusion: This study indicated potential advantages of the peptide-conjugated liposomes in drug transport to the cancer cells. This feature might be an outcome of probable interactions between the targeted nanoliposomes with the integrin and neuropilin-1 receptors.

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“…A bicelle has a flat bilayer disk with a rim, which is mainly formed by long-chain phospholipids and hydrophilic cosurfactants, respectively [8]. Alternative formulations of bicelles are also possible by mixtures of phospholipids and hydrophilic cosurfactants such as cholesterol sulfate [9], dodecyltrimethylammonium chloride (DTAC) [10], and polyoxyethylene sorbitan monooleate (Tween 80) [11,12]. Nonionic surfactants, such as Tween 80, which has much lower critical micelle concentrations than short-chain phospholipids or other ionic cosurfactants, are beneficial for their resistance to dilution as well as for efficient bicelle formulation.…”

Section: Introductionmentioning

confidence: 99%

Formulation of Bicelles Based on Lecithin-Nonionic Surfactant Mixtures

et al. 2020

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Bicelles have been intensively studied for use as drug delivery carriers and in biological studies, but their preparation with low-cost materials and via a simple process would allow their use for other purposes as well. Herein, bicelles were prepared through a semi-spontaneous method using a mixture of hydrogenated soybean lecithin (SL) and a nonionic surfactant, polyoxyethylene cholesteryl ether (ChEO10), and then we investigated the effect of composition and temperature on the structure of bicelles, which is important to design tailored systems. As the fraction of ChEO10 (XC) was increased, a bimodal particle size distribution with a small particle size of several tens of nanometers and a large particle size of several hundred nanometers was obtained, and only small particles were observed when XC ≥ 0.6, suggesting the formation of significant structure transition (liposomes to bicelles). The small-angle neutron scattering (SANS) spectrum for these particles fitted a core-shell bicelle model, providing further evidence of bicelle formation. A transition from a monomodal to a bimodal size distribution occurred as the temperature was increased, with this transition taking place at lower temperatures when higher SL-ChEO10 concentrations were used. SANS showed that this temperature-dependent size change was reversible, suggesting the SL-ChEO10 bicelles were stable against temperature, hence making them suitable for several applications.

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Structural analysis of “flexible” liposome formulations: new insights into the skin-penetrating ability of soft nanostructures

Cited by 50 publications

References 33 publications

Practical applications of small-angle neutron scattering

Practical applications of small-angle neutron scattering

Curcumin-loaded nanoliposomes linked to homing peptides for integrin targeting and neuropilin-1-mediated internalization

Formulation of Bicelles Based on Lecithin-Nonionic Surfactant Mixtures

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