Structural analysis of cell wall mannan of Candida sojae, a new yeast species isolated from defatted soybean flakes

Abstract

We investigated the structural and immunochemical characteristics of cell wall mannan obtained from Candida sojae JCM 1644, which is a new yeast species isolated from defatted soybean flakes. The results of a slide-agglutination test and of an enzyme-linked immunosorbent assay using anti-factor sera to the pathogenic Candida species indicated that the cells and the C. sojae mannan were cross-reactive to the specific anti-factor sera against Candida albicans serotype A (FAb 6) and Candida guilliermondii (FAb 9). Two-dimensional homonuclear Hartmann-Hahn analysis indicated that the mannan consisted of various linked oligomannosyl side chains containing alpha-1,2-, alpha-1,3-, alpha-1,6- and beta-1,2-linked mannose residues. However, although the determinants of antigenic factors 6 and 9 could be not found in this mannan, branched side chains, Manbeta1-2Manalpha1-3[Manalpha1-6]Manalpha1-(2Manalpha1-)n2Man and a linear alpha-1,6-linked polymannosyl backbone, which are cross-reacted by FAbs 6 and 9, respectively, were identified. The mannan was subjected to acetolysis in order to determine the polymerization length of the alpha-1,2-linked oligomannosyl residue in the side chains. The result of (1)H-nuclear magnetic resonance analysis of the released oligosaccharides showed that the remarkable regularity in the length of alpha-1,2-linked oligomannosyl side chains, which were previously found in mannans of other Candida species, is not observed in this mannan.

Identification of cell-surface mannans in a virulent Helicobacter pylori strain

“…Interestingly, the O-2 side chain residues were found to be either in aor in b-anomeric configuration. Similar mannans have been observed in cell surface of several other microorganisms, namely, in the cell wall of yeasts, 26,29,40 and fungi, 25 or in the form of phosphorylated mannans and arabinomannans in mycobacteria. 41 They have further been detected at the cell surface of Gram-positive bacteria, 27,42 as well as in exopolysaccharides from Gram-negative Pseudomonas syringae pv.…”

“…Combined data from 2D 1 H- 13 C HSQC experiments ( Fig. 3), linkage analysis (Table 1) and available literature [24][25][26][27] (Fig. 4).…”

“…24,27 The identified resonances are summarized in Table 2 and were found to be in accordance with the previous reports. 26 Additionally, NOESY experiments ( Fig. 5) (Table 1).…”

Structural Investigation of Cell Wall Mannan Antigen Obtained from Pathogenic Yeast <i>Candida zeylanoides</i>

“…Z was analyzed by means of 2D-HOHAHA (Figure 1). All H-1-H-2 crosspeaks were assigned according to published data [21][22][23][24][25] as shown in Table 2. The quartet signal corresponding peak 1 at 5.4 -5.7 ppm indicates the presence of the phosphorylated oligomannosyl side chain and β-1,2-linked oligomannosyl unit.…”

Streptococcus pneumoniae capsular polysaccharide is linked to peptidoglycan via a direct glycosidic bond to β-D- N -acetylglucosamine

“…Details for enzyme digestions, HF treatment, sodium borodeuteride reduction, Smith degradation (29), and acetolysis (as in ref. 40 with minor modifications) are provided in SI Materials and Methods. High and low MW products were separated using ultrafiltration (Amicon; 3,000 Da cutoff).…”