Structural analysis of broiler chicken small intestinal mucin O-glycan modification by Clostridium perfringens

MacMillan, Jaclyn L.; Vicaretti, Sara D.; Noyovitz, Benjamin; Xing, Xiaohui; Low, Kristin E.; Inglis, G. Douglas; Zaytsoff, Sarah J. M.; Boraston, A.B.; Smith, Steven P.; Uwiera, Richard R. E.; Selinger, L. Brent; Zandberg, Wesley F.; Abbott, D. Wade

doi:10.3382/ps/pez297

Cited by 25 publications

(16 citation statements)

References 85 publications

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“…As for the architecture of intestinal mucosa, we obtained results comparable to other studies in which villi, crypts, or both were positively influenced by yeast supplementation in diets for broilers [60][61][62]. The higher number of PAS positive mucin producers cells is related in the literature to a protective effects for the intestinal mucosa [63]. In the present study, the absence of significant effects in their counts could be related to the intrinsic characteristics of the Campylobacter, which usually develop as an asymptomatic infection in poultry.…”

Section: Discussionsupporting

confidence: 85%

Dietary Saccharomyces cerevisiae boulardii CNCM I-1079 Positively Affects Performance and Intestinal Ecosystem in Broilers during a Campylobacter jejuni Infection

et al. 2019

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In poultry production, probiotics have shown promise to limit campylobacteriosis at the farm level, the most commonly reported zoonosis in Europe. The aim of this trial was to evaluate the effects of Saccharomyces supplementation in Campylobacter jejuni challenged chickens on performance and intestinal ecosystem. A total of 156 day old male Ross 308 chicks were assigned to a basal control diet (C) or to a Saccharomyces cerevisiae boulardii CNCM I-1079 supplemented diet (S). All the birds were orally challenged with C. jejuni on day (d) 21. Live weight and growth performance were evaluated on days 1, 21, 28 and 40. The histology of intestinal mucosa was analyzed and the gut microbiota composition was assessed by 16S rRNA. Performance throughout the trial as well as villi length and crypt depth were positively influenced by yeast supplementation. A higher abundance of operational taxonomic units (OTUs) annotated as Lactobacillus reuteri and Faecalibacterium prausnitzii and a lower abundance of Campylobacter in fecal samples from S compared to the C group were reported. Supplementation with Saccharomyces cerevisiae boulardii can effectively modulate the intestinal ecosystem, leading to a higher abundance of beneficial microorganisms and modifying the intestinal mucosa architecture, with a subsequent improvement of the broilers’ growth performance.

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Section: Discussionsupporting

confidence: 85%

Dietary Saccharomyces cerevisiae boulardii CNCM I-1079 Positively Affects Performance and Intestinal Ecosystem in Broilers during a Campylobacter jejuni Infection

et al. 2019

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“…The probiotic supplementation induced an increase of the number of PNA-positive GCs in duodenum and caecum as well as an increase in Galβ1,3GalNAc content (PNA staining intensity) in duodenum and ileum. Since galactose and GalNAc constituting O-linked mucus in the small intestine of Gallus gallus domesticus are a carbon source for bacteria [64], these results suggest that the used probiotics could increase the energy source for the intestinal microbiota of guineafowls.…”

Section: Discussionmentioning

confidence: 95%

“…A higher secretion of intestinal sialomucins has been related to improved defense in mammals [63]. It has been found that sialic acid is preferentially removed by Clostridium perfringes when this opportunistic pathogen, responsible for necrotic enteritis in poultry, was incubated with the mucin O-linked glycans from the small intestine of Gallus gallus domesticus [64].…”

Section: Discussionmentioning

confidence: 99%

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Modulation of Morphology and Glycan Composition of Mucins in Farmed Guinea Fowl (Numida meleagris) Intestine by the Multi-Strain Probiotic Slab51®

Desantis

Galosi

Santamaría

et al. 2021

Animals

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Probiotics have become highly recognized as supplements for poultry.Since gut health can be considered synonymous withanimal health, the effects of probiotic Slab51® on the morphology and the glycan composition of guineafowlintestine were examined. The probiotics were added in drinking water (2 × 1011 UFC/L) throughout the grow-out cycle.Birds were individually weighed andslaughtered after four months. Samples from the duodenum, ileum and caecum were collected and processed for morphological, morphometric, conventional and lectin glycohistochemical studies.The results were analyzed for statistical significance by Student’s t test. Compared with control samples, probiotic group revealed (1) significant increase in villus height (p < 0.001 in duodenum and ileum; p < 0.05 in caecum), crypt depth (p < 0.001 in duodenum and caecum;p < 0.05 in ileum) and goblet cells (GCs) per villus (p < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans. Overall, dietary Slab51® induces morphological and region-specific changes in glycoprotein composition of guinea fowl intestine, promoting gut health.

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“…For example, C. perfringens harbors several carbohydrate-active enzymes that can function to degrade glycans of intestinal mucus [ 37 ]. Furthermore, a virulent C. perfringens strain (i.e., CP1) has been shown to utilize sialic acid of chicken intestinal mucus and may be a contributing function in the development of NE in poultry [ 48 ]. A high protein diet has been proposed as a predisposing factor to C. perfringens overgrowth, as the bacterium lacks genes for several amino acid synthesis pathways [ 49 , 50 ].…”

Section: Discussionmentioning

confidence: 99%

Physiological Stress Mediated by Corticosterone Administration Alters Intestinal Bacterial Communities and Increases the Relative Abundance of Clostridium perfringens in the Small Intestine of Chickens

2020

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A model of physiological stress mediated by the administration of corticosterone (CORT) was used to investigate the impact of stress on the intestinal microbiota of chickens. Birds were administered CORT in their drinking water at 0, 10 (low dose CORT; LDC), and 30 (high dose CORT; HDC) mg/L. Digesta from the small intestine and ceca were examined after 1, 5, and 12 days post-initiation of CORT administration by 16S rRNA gene sequencing. A decrease in phylogenetic diversity and altered composition of bacteria were observed for HDC in the small intestine. Analysis by ANOVA-Like Differential Expression 2 (ALDEx2) showed that densities of Clostridium sensu stricto 1 bacteria were increased in the small intestine for LDC and HDC. Quantitative PCR confirmed that CORT administration increased densities of Clostridium perfringens in the small intestine, but only HDC was associated with increased densities of the bacterium in ceca. Predictive functional analysis by Phylogenetic Investigation of Communities by Reconstruction of Unobserved States 2 (PICRUSt2) showed pathways of carbohydrate metabolism to be enriched with CORT, and amino acid synthesis to be enriched in control birds in the small intestine. In conclusion, physiological stress mediated by CORT modulated bacterial communities in the small intestine and increased densities of C. perfringens. This implicates stress as an important mediator of this important enteric pathogen in poultry.

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Structural analysis of broiler chicken small intestinal mucin O-glycan modification by Clostridium perfringens

Cited by 25 publications

References 85 publications

Dietary Saccharomyces cerevisiae boulardii CNCM I-1079 Positively Affects Performance and Intestinal Ecosystem in Broilers during a Campylobacter jejuni Infection

Dietary Saccharomyces cerevisiae boulardii CNCM I-1079 Positively Affects Performance and Intestinal Ecosystem in Broilers during a Campylobacter jejuni Infection

Modulation of Morphology and Glycan Composition of Mucins in Farmed Guinea Fowl (Numida meleagris) Intestine by the Multi-Strain Probiotic Slab51®

Physiological Stress Mediated by Corticosterone Administration Alters Intestinal Bacterial Communities and Increases the Relative Abundance of Clostridium perfringens in the Small Intestine of Chickens

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