Structural Analysis of Animal Virus Genomes: The Fifth Fleming Lecture

McGeoch, Duncan J.

doi:10.1099/0022-1317-55-1-1

Cited by 5 publications

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“…This unadecamer is followed by the dinucleotide GA (or CA in the case of the NS : M junction), which is untranscribed and marks the intercistronic junction. These dinucleotide spacers are followed immediately by sequences with the general form (5')-AACAGNNAUC-(3') complementary to the 5' end of the mRNA, where N represents a variable site (McGeoch, 1979(McGeoch, , 1981. At the 5' end of the genome, there is an untranscribed region of 59 nucleotides beyond the 3' terminus of the L gene.…”

Section: Intercistronic Junctionsmentioning

confidence: 99%

“…The five mRNAs are capped, methylated, and polyadenylated, whereas the leader RNA is not. The 3'-terminal adjacent region is conserved in at least five vesiculoviruses (McGeoch, 1981), although the polymerasebinding site located at position 16-30 in the middle of the leader region (Keene et al, 1981b) does not appear to be. The precise function of the leader RNA is unclear, but plus-strand leader appears to be involved in the inhibition of cellular RNA synthesis, because in vitro VSV leader RNA or its cDNA inhibited transcription of adenovirus by poIn and polIII (McGowan et al, 1982;Grinnell and Wagner, 1984).…”

Section: Gene Productsmentioning

confidence: 99%

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Rhabdovirus Genetics

Pringle¹

1987

The Rhabdoviruses

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There are five families of RNA viruses in which the negative strand is sequestered in the extracellular virion. Viruses of two of these families, the Rhabdoviridae and the Paramyxoviridae, have unitary linear genomes, whereas viruses of the other three families, Arenaviridae, Bunyaviridae, and Orthomyxoviridae, have segmented genomes comprising, respectively, two, three, and seven or eight subunits. The informational macromolecules that comprise the genomes of rhabdoviruses and paramyxoviruses are among the largest functional RNA molecules and are exceeded in size only by those of the plus-strand coronaviruses. Reanney (1982Reanney ( , 1984 has calculated that the upper size limit for any RNA virus genome cannot be much in excess of 17,600 nucleotides (mol. wt. =5.7 x 10 6 ) as a consequence of the low copying fidelity of RNA polymerases. The segmentation of the genomes of the other negative-strand viruses may be a consequence of such constraints on molecular size or a device for decoupling the transcription of !ndividual genes. Whatever the reason, the genetic properties of the segmented-genome viruses differ substantially from those of the unsegmented-genome viruses, because variation in the former is generated by reassortment of genome subunits as well as by mutation. Mutation is the sole mechanism of variation in unsegmented-genome viruses, since the intramolecular recombination observed with positive-

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Section: Intercistronic Junctionsmentioning

confidence: 99%