Structural analysis and immunoregulation activity comparison of five polysaccharides from Angelica sinensis

Wang, Junmin; Ge, Beilei; Li, Zihong; Guan, Fangxia; Li, Feifei

doi:10.1016/j.carbpol.2015.12.050

Cited by 78 publications

(38 citation statements)

References 26 publications

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“…Increasing evidence has indicated that fungi polysaccharides possess various biological activities, including anti-tumor,4 anti-inflammatory,5 and immunopotentiation6 functions. Fungi polysaccharides, with no or poor toxicity, have attracted much attention in medical research.…”

Section: Introductionmentioning

confidence: 99%

Metabonomic Variation of Exopolysaccharide from Rhizopus nigricans on AOM/DSS-Induced Colorectal Cancer in Mice

Lü¹,

Wang²,

Ji³

et al. 2019

OTT

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BackgroundColorectal cancer (CRC), which occurs at the junction of the rectum and sigmoid colon, is a common malignancy associated with poor prognosis and high mortality worldwide. The exopolysaccharide (EPS1-1), isolated from the fermentation broth of Rhizopus nigricans (R. nigricans), has been reported to possess anti-CRC properties. However, the metabolic alterations caused by azoxymethane (AOM) and dextran sulfate sodium (DSS) are still unknown.MethodsIn the present study, a mice colon cancer model was established by treatment with AOM/DSS. LC-MS/MS-based metabolomics studies were performed to analyze metabolic alterations at the tissue level. Partial least squares discriminant analysis (PLS-DA) was used to identify differentially expressed metabolites.ResultsNineteen distinct metabolites were identified that were associated with disruptions in the following pathways: biosynthesis of unsaturated fatty acids, pyrimidine metabolism, phenylalanine metabolism, fatty acid metabolism, folate biosynthesis, and inositol phosphate metabolism. Furthermore, six significantly altered metabolites were involved in these six pathways. Compared with the Model group, the expression of cytosine, deoxyuridine, 20-hydroxy-leukotriene E4, and L-homocysteic acid was lower, whereas that of 2-dehydro-3-deoxy-6-phospho-D-gluconic acid and hematoporphyrin was higher in the EPS1-1 group.ConclusionThe results of multivariate statistical analysis demonstrate a promising application of the above metabolites by EPS1-1 in CRC therapy. Deeper understanding of the related mechanism warrants further investigation.

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Section: Introductionmentioning

confidence: 99%

Metabonomic Variation of Exopolysaccharide from Rhizopus nigricans on AOM/DSS-Induced Colorectal Cancer in Mice

Lü¹,

Wang²,

Ji³

et al. 2019

OTT

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“…Angelica polysaccharide (AP), the major bioactive component of Angelica sinensis (Oliv) Diels, is a β- d -pyranoid polysaccharide with multiple biological activities, such as hematopoiesis, immunomodulation, anti-oxidation, anti-tumor, and radioprotection. 9,10 For example, Zhang et al 11 reported that AP could promote glioma cell apoptosis and inhibit cell growth both in vitro and in vivo. A previous literature has reported that AP possesses immunostimulatory effects on Pacific white shrimps.…”

Section: Introductionmentioning

confidence: 99%

RETRACTED: Possible treatment for cutaneous lichen planus: An in vitro anti-inflammatory role of Angelica polysaccharide in human keratinocytes HaCaT

Wang

Shi

Wang

et al. 2019

Int J Immunopathol Pharmacol

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Cutaneous lichen planus (CLP) is an autoimmune disease. Angelica polysaccharide (AP) has been found to exert immunomodulation activity. In this study, we explored the roles of AP in lipopolysaccharide (LPS)-induced inflammatory injury of human keratinocytes (HaCaT cells), as well as the underlying mechanisms. LPS-induced cell injury was evaluated by alterations of cell viability, apoptosis, and expressions of proteins associated with apoptosis and inflammatory cytokines. Then, the protective effects of AP on LPS-induced cell injury were assessed. The protein expressions of sirtuin 1 (SIRT1) and key kinases in the Nrf2/HO-1 and nuclear factor κB (NF-κB) pathways were measured using western blotting. SIRT1 knockdown and overexpression were used to analyze whether AP affected HaCaT cells through regulating SIRT1. Finally, the possible inhibitory effects of AP on cell injury after LPS treatment were also evaluated. We found that LPS reduced HaCaT cell viability, enhanced apoptosis, and induced release of inflammatory cytokines. AP alleviated LPS-induced HaCaT cell inflammatory injury. The expression of SIRT1 was enhanced after AP treatment. AP activated Nrf2/HO-1 pathway while inhibited NF-κB pathway in HaCaT cells. The protective effects of AP on LPS-induced HaCaT cell injury were reversed by SIRT1 knockdown. Dysregulation of SIRT1 altered the activation of Nrf2/HO-1 and NF-κB pathways in LPS-treated HaCaT cells. Furthermore, AP also exerted inhibitory effects on HaCaT cell injury after LPS stimulation. In conclusion, AP could alleviate LPS-induced inflammatory injury of HaCaT cells through upregulating SIRT1 expression and then activating Nrf2/HO-1 pathway but inactivating NF-κB pathway. This study provided a possible therapeutic strategy for clinical CLP treatments.

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“…It has been reported that natural polysaccharides exhibited a wide range of biological activities, especially antioxidant and antitumor activities (Ghazala et al, ; Kong et al, ). Till now, many plant polysaccharides, such as polysaccharides from Angelica sinensis and arabinoxylans from many cereal grains and Yupingfeng polysaccharides have been demonstrated to have beneficial effects on immunoregulation in vitro and in vivo (Mendis, Leclerc, & Simsek, ; Sun et al, ; Wang, Ge, Li, Guan, & Li, ).…”

Section: Introductionmentioning

confidence: 99%

Immunomodulatory effects of polysaccharides from purple sweet potato on lipopolysaccharide treated RAW 264.7 macrophages

et al. 2018

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Immunomodulatory effects of water-soluble polysaccharide (WSP), dilute alkali-soluble polysaccharide (DASP), and concentrated alkali-soluble polysaccharide (CASP) from purple sweet potato were evaluated by using lipopolysaccharide (LPS) treated RAW264.7 macrophage cell models. Results showed WSP, DASP, and CASP (100, 200, and 400 lg/mL) could suppress abnormal phagocytosis, overproduction of TNF-a and IL-6, and abnormal apoptosis caused by LPS. The high doses (200 and 400 lg/mL) of DASP and CASP could distinctly reduce excessive NO production as compared with LPS treatment group. In addition, 400 lg/mL of WSP, DASP, and CASP treatment groups exhibited dramatic decrease in the overexpression of IL-1b stimulated by LPS. Moreover, 200 and 400 lg/mL of WSP, DASP, and CASP markedly improved anti-inflammatory cytokine IL-10 as compared with LPS treatment group. Furthermore, 400 lg/mL of WSP and DASP treatments significantly increased the level of IL-10 in nonstimulated macrophages when compared with normal control group. Practical applicationsPurple sweet potato [Ipomoea batatas (L.) Lam] is one type of highly nutritional vegetable containing many vitamins, minerals, dietary fiber, anthocyanin, and polysaccharides. In addition, it is also one of health-promoting edible plant sources. Recently, polysaccharides have attracted extensive attentions due to their various biological activities. Our study reveals that polysaccharides extracted from purple sweet potato are effective in protecting macrophages damaged by lipopolysaccharide. Therefore, polysaccharides from purple sweet potato have anti-inflammatory efficacy.Thus, this study indicates the potential applications of purple sweet potato polysaccharides in functional food.

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Structural analysis and immunoregulation activity comparison of five polysaccharides from Angelica sinensis

Cited by 78 publications

References 26 publications

<p>Metabonomic Variation of Exopolysaccharide from <em>Rhizopus nigricans</em> on AOM/DSS-Induced Colorectal Cancer in Mice</p>

<p>Metabonomic Variation of Exopolysaccharide from <em>Rhizopus nigricans</em> on AOM/DSS-Induced Colorectal Cancer in Mice</p>

RETRACTED: Possible treatment for cutaneous lichen planus: An in vitro anti-inflammatory role of Angelica polysaccharide in human keratinocytes HaCaT

Immunomodulatory effects of polysaccharides from purple sweet potato on lipopolysaccharide treated RAW 264.7 macrophages

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