Structural alteration in hypochlorous acid modified antithrombin indicates generation of neo-epitopes

Ahmad, Parvez; Tantry, Irfan Qadir; Ali, Asif; Siddiqui, Shahid Ali; Rehman, Sufia; Waris, Sana; Jairajpuri, Mohamad Aman

doi:10.1016/j.abb.2020.108332

Cited by 4 publications

(1 citation statement)

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“…Depending on the degree of modification, oxidative damage can also lead to increased antibody binding. This was shown in several studies examining the effect of hypochlorous acid on extracellular matrix proteins [ 87 , 88 ]. The rather high sensitivity of immunological methods and easy-to-carry-out protocols boosted the number of biological studies working with immunoblotting or enzyme linked immunosorbent assay (ELISA).…”

Section: Analysis Of Oxidative Protein Modificationsmentioning

confidence: 68%

Protein oxidation - Formation mechanisms, detection and relevance as biomarkers in human diseases

et al. 2021

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Generation of reactive oxygen species and related oxidants is an inevitable consequence of life. Proteins are major targets for oxidation reactions, because of their rapid reaction rates with oxidants and their high abundance in cells, extracellular tissues, and body fluids. Additionally, oxidative stress is able to degrade lipids and carbohydrates to highly reactive intermediates, which eventually attack proteins at various functional sites. Consequently, a wide variety of distinct posttranslational protein modifications is formed by protein oxidation, glycoxidation, and lipoxidation. Reversible modifications are relevant in physiological processes and constitute signaling mechanisms (“redox signaling”), while non-reversible modifications may contribute to pathological situations and several diseases. A rising number of publications provide evidence for their involvement in the onset and progression of diseases as well as aging processes. Certain protein oxidation products are chemically stable and formed in large quantity, which makes them promising candidates to become biomarkers of oxidative damage. Moreover, progress in the development of detection and quantification methods facilitates analysis time and effort and contributes to their future applicability in clinical routine. The present review outlines the most important classes and selected examples of oxidative protein modifications, elucidates the chemistry beyond their formation and discusses available methods for detection and analysis. Furthermore, the relevance and potential of protein modifications as biomarkers in the context of disease and aging is summarized.

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Section: Analysis Of Oxidative Protein Modificationsmentioning

confidence: 68%