Strontium- and cobalt-substituted bioactive glasses seeded with human umbilical cord perivascular cells to promote bone regeneration via enhanced osteogenic and angiogenic activities

Kargozar, Saeid; Lotfibakhshaiesh, Nasrin; Ai, Jafar; Mozafari, Masoud; Milan, Peiman Brouki; Hamzehlou, Sepideh; Barati, Mahmood; Baino, Francesco; Hill, Robert G.; Joghataei, Mohammad Taghi

doi:10.1016/j.actbio.2017.06.021

Cited by 146 publications

(75 citation statements)

References 45 publications

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“…Cobalt doped bioactive glasses have been widely studied as hypoxia mimicking biomaterials for bone tissue regeneration, however, the majority of these studies have been in silicate based glasses [23,[30][31][32][33][34][35][36][37]. Whilst many studies have been undertaken to elucidate the antimicrobial potential of various glass dissolution with an increasing metal oxide content [11,12,38].…”

Section: Discussionmentioning

confidence: 99%

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The Antimicrobial Efficacy of Hypoxia Mimicking Cobalt Oxide Doped Phosphate-Based Glasses against Clinically Relevant Gram Positive, Gram Negative Bacteria and a Fungal Strain

Raja

Worthington

Isaacs

et al. 2018

ACS Biomater. Sci. Eng.

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Bioactive phosphate glasses are of considerable interest for a range of soft and hard tissue engineering applications. The glasses are degradable and can release biologically important ions in a controlled manner. The glasses can also potentially be used as an antimicrobial delivery system. In the given study, novel cobalt doped phosphate-based glasses, (P O 5) 50 (Na 2 O) 20 (CaO) 30-x (CoO) x where 0 ≤ x (mol%) ≤ 10, were manufactured and characterised. As the cobalt oxide concentration increased the rate of dissolution was observed to decrease. The antimicrobial potential of the glasses was studied using direct and indirect contact methods against both Escherichia coli (NCTC 10538) Staphylococcus aureus (ATCC 6538) and Candida albicans (ATCC 76615). The results showed a strong, time dependent and strain specific, antimicrobial activity of the glasses against microorganisms when in direct contact. Antimicrobial activity (R) ≥ 2 was observed within 2 hours against Escherichia coli whereas similar effect was achieved in 6 hours against Staphylococcus aureus and Candida albicans. However, when in indirect contact, the dissolution products from the bioactive glasses failed to show antimicrobial effect. Following direct exposure to the glasses for 7 days, osteoblast-like SAOS-2 cells showed a 5-fold increase in VEGF mRNA whilst THP-1 monocytic cells showed a 4-fold increase in VEGF mRNA expression when exposed to 10% CoO doped glass compared with the cobalt free control glass. Endothelial cells stimulated with conditioned medium taken from cell cultures of THP-1 monocytes exposed to 10% CoO doped glass showed clear tube-like structure (blood vessel) formation after 4 hours.

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Section: Discussionmentioning

confidence: 99%

“…To date the majority of hypoxia mimicking glasses have focused on silicate based glasses [23,[30][31][32][33][34][35][36][37]. Relative few studies have been undertaken on cobalt doped phosphate glasses and studies that have been undertaken were co-doped with titanium dioxide [40,41].…”

Section: Discussionmentioning

confidence: 99%

The Antimicrobial Efficacy of Hypoxia Mimicking Cobalt Oxide Doped Phosphate-Based Glasses against Clinically Relevant Gram Positive, Gram Negative Bacteria and a Fungal Strain

Raja

Worthington

Isaacs

et al. 2018

ACS Biomater. Sci. Eng.

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“…It has previously been well documented that strontium is an osteogenesis-inducing element which could be easily substituted with calcium in the BG network [16,17]. Strontium could enhance the bone density through increase in the osteoblast activity and inhibition of osteoclast function (anti-osteoporotic effect) [18,19].…”

Section: Introductionmentioning

confidence: 99%

Strontium- and Cobalt-Doped Multicomponent Mesoporous Bioactive Glasses (MBGs) for Potential Use in Bone Tissue Engineering Applications

et al. 2020

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Mesoporous bioactive glasses (MBGs) offer suitable platforms for drug/ion delivery in tissue engineering strategies. The main goal of this study was to prepare strontium (Sr)- and cobalt (Co)-doped MBGs; strontium is currently used in the treatment of osteoporosis, and cobalt is known to exhibit pro-angiogenic effects. Sr- and Co-doped mesoporous glasses were synthesized for the first time in a multicomponent silicate system via the sol–gel method by using P123 as a structure-directing agent. The glassy state of the Sr- and Co-doped materials was confirmed by XRD before immersion in SBF, while an apatite-like layer was detected onto the surface of samples post-immersion. The textural characteristics of MBGs were confirmed by nitrogen adsorption/desorption measurements. In vitro experiments including MTT assay, Alizarin red staining, and cell attachment and migration showed the cytocompatibility of all the samples as well as their positive effects on osteoblast-like cell line MG-63. Early experiments with human umbilical vein endothelial cells also suggested the potential of these MBGs in the context of angiogenesis. In conclusion, the prepared materials were bioactive, showed the ability to improve osteoblast cell function in vitro and could be considered as valuable delivery vehicles for therapeutics, like Co2+ and Sr2+ ions.

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“…The simultaneous effect of applying Sr and Co ions on the acceleration of bone healing and vascularization was further con rmed by implanting Sr-Co-BG seeded with human umbilical cord perivascular cells (HUCPVCs) in the knee defect of the rabbits for 12 weeks. The results revealed signi cant improved angiogenic and regeneration potential of BGs after being doped with Sr and Co [25]. Previously, our group demonstrated that BG/Sr containing scaffolds promote proliferation and osteogenic differentiation of Mesenchymal Stem Cells (MSCs) as well as angiogenesis [21].…”

Section: Introductionmentioning

confidence: 83%

Effects of strontium ions with potential antibacterial activity on in vivo bone regeneration

Baheiraei

Eyni

Bakhshi

et al. 2020

Preprint

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Background: Bioactive glasses (BGs) have attracted added attention in the structure of the scaffolds for bone repair applications. Different metal ions could be doped in BGs to induce specific biological responses. Among these ions, strontium (Sr) is considered as an effective and safe doping element with promising effects on bone formation and regeneration.Methods: In this experiment, we evaluated the antibacterial activities of the gelatin-BG (Gel-BG) and Gel-BG/Sr scaffolds in vitro. The osteogenic properties of the prepared scaffolds were also assessed in rabbit calvarial bone defects for 12 weeks. Alizarin Red, Hematoxylin & Eosin (H&E) and Masson’s Trichrome staining were performed to assess bone regeneration and the obtained results were compared with those without Sr. Also, histomorphometric data were obtained to evaluate the new bone, residual graft, and connective tissue.Results: Both scaffolds showed in vivo bone formation during 12 weeks with the newly formed bone area in Gel-BG/Sr scaffold was higher than that in Gel-BG scaffolds after the whole period. Based on the histological results, Gel-BG/Sr exhibited acceleration of early-stage bone formation in vivo. The results of antibacterial investigation showed that although both Gel-BG/Sr and Gel-BG effectively inhibited the growth of Escherichia coli (E. coli) but, only Gel-BG/Sr structure could lead to a 3 log reduction in Staphylococcus aureus (S. aureus). Conclusions: Our results confirmed that Sr doped BG is a favorable candidate for bone tissue engineering with superior antibacterial activity and bone regeneration capacity compared with similar counterparts having no Sr ion.

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Strontium- and cobalt-substituted bioactive glasses seeded with human umbilical cord perivascular cells to promote bone regeneration via enhanced osteogenic and angiogenic activities

Cited by 146 publications

References 45 publications

The Antimicrobial Efficacy of Hypoxia Mimicking Cobalt Oxide Doped Phosphate-Based Glasses against Clinically Relevant Gram Positive, Gram Negative Bacteria and a Fungal Strain

The Antimicrobial Efficacy of Hypoxia Mimicking Cobalt Oxide Doped Phosphate-Based Glasses against Clinically Relevant Gram Positive, Gram Negative Bacteria and a Fungal Strain

Strontium- and Cobalt-Doped Multicomponent Mesoporous Bioactive Glasses (MBGs) for Potential Use in Bone Tissue Engineering Applications

Effects of strontium ions with potential antibacterial activity on in vivo bone regeneration

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