Strongly Enhanced Serum Levels of Vascular Endothelial Growth Factor (VEGF) after Poly-trauma and Burn

Grad, Sibylle; Ertel, Wolfgang; Keel, Marius; Infanger, Manfred; Vonderschmitt, Dieter J.; Maly, Friedrich E.

doi:10.1515/cclm.1998.064

Cited by 61 publications

(55 citation statements)

References 21 publications

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“…In addition, the young age of our sample population could account for some difference. Vascular endothelial growth factor concentrations can be raised by all manner of pathological processes from trauma to liver disease to pre-eclampsia (Iitaka et al, 1998;Akiyoshi et al, 1998;Baker et al, 1995;Grad et al, 1998). In a population with an older mean age, you could reasonably expect to find a cross section of pathology that is not present in our study population, thus giving our lower plasma concentrations.…”

Section: Molecular and Cellular Pathologymentioning

confidence: 79%

A comparison of serum and plasma levels of vascular endothelial growth factor during the menstrual cycle in healthy female volunteers

2002

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Angiogenesis is the formation of new blood vessels from the existing vasculature, and is essential for the growth and metastasis of most solid tumours. One of the most important growth factors involved in the angiogenesis process is vascular endothelial growth factor. Vascular endothelial growth factor expression has been shown to be regulated by female hormones in breast cancer cell lines, and two previous authors have reported on cyclical variations in serum vascular endothelial growth factor concentrations with conflicting results. No work has been performed on variations in plasma levels of vascular endothelial growth factor during the menstrual cycle. We therefore conducted the first prospective trial to compare serum and plasma levels of vascular endothelial growth factor in healthy pre-menopausal volunteers. Twenty healthy pre-menopausal women were recruited and had blood samples taken over one menstrual cycle with an average of eight samples taken per patient. Plasma and serum samples were then analysed for sex hormones and vascular endothelial growth factor 165. Serum vascular endothelial growth factor levels were found to be significantly higher than plasma vascular endothelial growth factor levels (P50.005). We found no significant difference between serum and plasma vascular endothelial growth factor in the luteal and follicular phases of the cycle. The majority of the measurements for plasma levels of vascular endothelial growth factor at all phases of the cycle were under the limit of detection of the vascular endothelial growth factor ELISA kit. We found no significant correlation between plasma or serum levels of vascular endothelial growth factor and either FSH, LH, Oestradiol or Progesterone levels. This study has demonstrated no difference in serum concentrations of vascular endothelial growth factor during the different phases of the menstrual cycle in a group of healthy volunteers. We also demonstrated no obvious difference in plasma concentrations of vascular endothelial growth factor between the phases of the cycle, but most of the measurements were below the level of accuracy reported by the ELISA kit manufacturer. With the sensitivity of this ELISA test, therefore, we must still regard the question of whether there is a variation in plasma concentrations of vascular endothelial growth factor throughout the menstrual cycle as unanswered.

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Section: Molecular and Cellular Pathologymentioning

confidence: 79%

A comparison of serum and plasma levels of vascular endothelial growth factor during the menstrual cycle in healthy female volunteers

2002

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“…Our results indicate that necrotic cells also may function as inducers of metalloproteinase expression and thus directly initiate a wound-healing response after tissue damage. VEGF is an important mediator of angiogenesis, another key component of the tissue-repair pathway (12). Interestingly, VEGF expression could be detected in areas adjacent to necrotic cells in various disease models (13,14) and in situ analysis of tumor specimens also has demonstrated the localization of VEGF-producing cells proximal to necrotic foci (39).…”

Section: Discussionmentioning

confidence: 99%

“…Transcripts encoding stromelysin-1/MMP3 and VEGF, two proteins which play an important role in tissue repair (12,31), also were induced by necrotic cells in RelA ϩ/Ϫ MEFs, whereas induction was reduced in RelA Ϫ/Ϫ MEFs (Fig. 3A).…”

Section: Inflammatory and Tissue-repair Gene Induction In Mefs And Mamentioning

confidence: 97%

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An Essential Role of the NF-κB/Toll-Like Receptor Pathway in Induction of Inflammatory and Tissue-Repair Gene Expression by Necrotic Cells

Carpio²,

Zheng³

et al. 2001

The Journal of Immunology

411

291

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Tissue damage induced by infection or injury can result in necrosis, a mode of cell death characterized by induction of an inflammatory response. In contrast, cells dying by apoptosis do not induce inflammation. However, the reasons for underlying differences between these two modes of cell death in inducing inflammation are not known. Here we show that necrotic cells, but not apoptotic cells, activate NF-κB and induce expression of genes involved in inflammatory and tissue-repair responses, including neutrophil-specific chemokine genes KC and macrophage-inflammatory protein-2, in viable fibroblasts and macrophages. Intriguingly, NF-κB activation by necrotic cells was dependent on Toll-like receptor 2, a signaling pathway that induces inflammation in response to microbial agents. These results have identified a novel mechanism by which cell necrosis, but not apoptosis, can induce expression of genes involved in inflammation and tissue-repair responses. Furthermore, these results also demonstrate that the NF-κB/Toll-like receptor 2 pathway can be activated both by exogenous microbial agents and endogenous inflammatory stimuli.

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“…Atypically severe orf virus lesions have been reported for thermally injured skin (20), and erythema multiforme reactions are a relatively common complication of orf virus infection. Intriguingly, both of these pathologies, in the absence of orf virus, have been linked to elevated levels of VEGF (6,17).…”

Section: Discussionmentioning

confidence: 99%

Viral Vascular Endothelial Growth Factor Plays a Critical Role in Orf Virus Infection

Savory¹,

Stacker

Fleming³

et al. 2000

J Virol

125

116

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Infection by the parapoxvirus orf virus causes proliferative skin lesions in which extensive capillary proliferation and dilation are prominent histological features. This infective phenotype may be linked to a unique virus-encoded factor, a distinctive new member of the vascular endothelial growth factor (VEGF) family of molecules. We constructed a recombinant orf virus in which the VEGF-like gene was disrupted and show that inactivation of this gene resulted in the loss of three VEGF activities expressed by the parent virus: mitogenesis of vascular endothelial cells, induction of vascular permeability, and activation of VEGF receptor 2. We used the recombinant orf virus to assess the contribution of the viral VEGF to the vascular response seen during orf virus infection of skin. Our results demonstrate that the viral VEGF, while recognizing a unique profile of the known VEGF receptors (receptor 2 and neuropilin 1), is able to stimulate a striking proliferation of blood vessels in the dermis underlying the site of infection. Furthermore, the data demonstrate that the viral VEGF participates in promoting a distinctive pattern of epidermal proliferation. Loss of a functional viral VEGF resulted in lesions with markedly reduced clinical indications of infection. However, viral replication in the early stages of infection was not impaired, and only at later times did it appear that replication of the recombinant virus might be reduced.

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Strongly Enhanced Serum Levels of Vascular Endothelial Growth Factor (VEGF) after Poly-trauma and Burn

Cited by 61 publications

References 21 publications

A comparison of serum and plasma levels of vascular endothelial growth factor during the menstrual cycle in healthy female volunteers

A comparison of serum and plasma levels of vascular endothelial growth factor during the menstrual cycle in healthy female volunteers

An Essential Role of the NF-κB/Toll-Like Receptor Pathway in Induction of Inflammatory and Tissue-Repair Gene Expression by Necrotic Cells

Viral Vascular Endothelial Growth Factor Plays a Critical Role in Orf Virus Infection

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