Strong-light-induced yellowing of green microalgae Chlorella: A study on molecular mechanisms of the acclimation response

Grudziński, Wojciech; Krzemińska, Izabela; Luchowski, Rafał; Nosalewicz, Artur; Gruszecki, Wiesław I.

doi:10.1016/j.algal.2016.03.021

Cited by 25 publications

(12 citation statements)

References 37 publications

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“…Photo‐acclimation occurred for the stress of strong light intensity, resulting in the change of biochemical composition of microalgae . For the light intensity of 400 µmol photons m −2 s −1 , Chlorella protothecoides and C. vulgaris turn yellow, compared with the green control cells cultured at low light intensity (80 µmol photons m −2 s −1 ) . The fact in our study that the chlorophyll concentration was slightly lower for 60 µmol m −2 s −1 than that for 80 µmol m −2 s −1 also confirmed this point.…”

Section: Resultssupporting

confidence: 82%

“…Actually, light intensity also greatly affects the composition of microalgae. Photo‐acclimation occurred for the stress of strong light intensity, resulting in the change of biochemical composition of microalgae . For the light intensity of 400 µmol photons m −2 s −1 , Chlorella protothecoides and C. vulgaris turn yellow, compared with the green control cells cultured at low light intensity (80 µmol photons m −2 s −1 ) .…”

Section: Resultsmentioning

confidence: 99%

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Influence of light intensity on microalgal growth, nutrients removal and capture of carbon in the wastewater under intermittent supply of CO₂

Liu

Ying

Chen³

et al. 2018

J of Chemical Tech & Biotech

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BACKGROUND: The role of light intensity in microalgal cultivation supplied with CO 2 is still required to be fully investigated. This study researched the growth of Chlorella vulgaris (C. vulgaris), nutrient removal and carbon capture in the wastewater for various light intensities under the intermittent injection of CO 2 . RESULTS: The biomass for 80 mol m −2 s −1 reached maximum value within 6 days. The chlorophyll content of C. vulgaris exhibited an ascending trend when increasing the light intensity from 20 to 60 mol m −2 s −1 . Cultural pH for 80 mol m −2 s −1 showed the largest level of increase in the first 4 days, then began to decline. The light intensity of 80 mol m −2 s −1 had the quickest TN and TP reduction trend in the wastewater. The difference in consumed N versus P ratio was caused by the different specific growth rates and demands for nutrients at each growth stage of C. vulgaris. The degradation of tryptophan-like substances and the increase of other substances were likely due to the release of extracellular polymeric substance of C. vulgaris. CONCLUSION: The light intensity of 80 mol m −2 s −1 resulted in the highest biomass, the quickest TN and TP reduction trend in the wastewater. Light intensity exhibited no significant influence on removal of organic carbon, but affected the transformation of organic compounds in the wastewater under intermittent supply of 10% CO 2 . RESULTS AND DISCUSSION BiomassThe biomass of C. vulgaris presented an ascending trend when increasing the light intensity from 20 to 60 μmol m −2 s −1 , as described in Fig. 1(a). However, no significant differences in biomass were observed between 60 and 80 μmol m −2 s −1 . The biomass for 80 μmol m −2 s −1 reached a plateau within 6 days, being 1.04 g L −1 , whereas the plateaus (0.65, 0.95, 1.03 and 0.95 g L −1 ) were reached within 6.5 days for 20, 40, 60 μmol m −2 s −1 and gradual increase treatments. Thus, C. vulgaris attained the maximum biomass for various light intensities at 6 or 6.5 days. Higher light intensity resulted in an increase in maximum biomass concentration achievable, but when the light intensity was above a certain threshold value, the highest biomass achievable become lower. The result was in agreement with the finding J Chem Technol Biotechnol 2018; 93: [3582][3583][3584][3585][3586][3587][3588][3589]

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Section: Resultssupporting

confidence: 82%

Section: Resultsmentioning

confidence: 99%

Influence of light intensity on microalgal growth, nutrients removal and capture of carbon in the wastewater under intermittent supply of CO₂

Liu

Ying

Chen³

et al. 2018

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

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“…( 1) where: N 1 is the initial biomass concentration at time t 1 and N 2 is the biomass concentration at time t 2 (Grudziński et al, 2016).…”

Section: Methodsmentioning

confidence: 99%

Stimulatory effect of indole-3-acetic acid and continuous illumination on the growth of Parachlorella kessleri

Magierek¹,

Krzemińska²,

Tys³

2017

International Agrophysics

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A b s t r a c t. The effects of the phytohormone indole-3-acetic acid and various conditions of illumination on the growth of Parachlorella kessleri were investigated. Two variants of illumination: continuous and photoperiod 16/8 h (light/dark) and two concentrations of the phytohormone -10 -4 M and 10 -5 M of indole-3-acetic acid were used in the experiment. The results of this study show that the addition of the higher concentration of indole-3-acetic acid stimulated the growth of P. kessleri more efficiently than the addition of the lower concentration of indole-3-acetic acid. This dependence can be observed in both variants of illumination. Increased biomass productivity was observed in the photoperiod conditions. Both the addition of the phytohormone and the conditions of the illumination had an impact on the number of P. kessleri cells. An increased number of cells was observed under the conditions of continuous illumination. This result has shown that the continuous illumination and the higher concentration of the phytohormone stimulated the growth of P. kessleri more effectively than the shorter duration of light (16/8 h (light/dark)).K e y w o r d s: light, photoperiod, microalgae, indole-3-acetic acid, Parachlorella kessleri

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“…Microalgae synthesise many valuable metabolites, e.g. pigments, carbohydrates, amino acids and fatty acids, which are attractive for commercial exploitation (Krzemińska et al 2015a; Grudziński et al 2016). Since these compounds are usually intracellularly located, cell disintegration prior to extraction thereof must be carried out (Postma et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Effectiveness of Parachlorella kessleri cell disruption evaluated with the use of laser light scattering methods

et al. 2018

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The main objective of this study is to demonstrate the possibilities of using laser light scattering methods, dynamic light scattering and laser Doppler electrophoresis, as suitable methods in investigations of algal production biosystems and biotechnology. This paper highlights the innovative use of the dynamic light scattering (DLS) methods for monitoring the destruction of Parachlorella kessleri cells. Additionally, these results indicate electrophoretic mobility as a new parameter to investigate the effectiveness of cell disruption prior to extraction conducted to optimise the biotechnological processes of recovery of microalgal intracellular metabolites. The efficacy of P. kessleri cell disintegration by ultrasound was determined by measurements of the number of cells with the algal cell reduction (CR ns), relative mean hydrodynamic diameter (Rd t) and electrophoretic mobility after applying different lengths of ultrasound exposure to a cell suspension. It was found that stationary-phase cells were the most resistant to the ultrasound treatment, especially at low values of the optical density. Both the relative hydrodynamic diameter and the electrophoretic mobility of cells were correlated statistically significantly with the time of sonication (t) and the algal cell reduction. The relationships allowed estimation of the sonication time needed for total cell disruption.

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Strong-light-induced yellowing of green microalgae Chlorella: A study on molecular mechanisms of the acclimation response

Cited by 25 publications

References 37 publications

Influence of light intensity on microalgal growth, nutrients removal and capture of carbon in the wastewater under intermittent supply of CO₂

Influence of light intensity on microalgal growth, nutrients removal and capture of carbon in the wastewater under intermittent supply of CO₂

Stimulatory effect of indole-3-acetic acid and continuous illumination on the growth of Parachlorella kessleri

Effectiveness of Parachlorella kessleri cell disruption evaluated with the use of laser light scattering methods

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Strong-light-induced yellowing of green microalgae Chlorella: A study on molecular mechanisms of the acclimation response

Cited by 25 publications

References 37 publications

Influence of light intensity on microalgal growth, nutrients removal and capture of carbon in the wastewater under intermittent supply of CO2

Influence of light intensity on microalgal growth, nutrients removal and capture of carbon in the wastewater under intermittent supply of CO2

Stimulatory effect of indole-3-acetic acid and continuous illumination on the growth of Parachlorella kessleri

Effectiveness of Parachlorella kessleri cell disruption evaluated with the use of laser light scattering methods

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Influence of light intensity on microalgal growth, nutrients removal and capture of carbon in the wastewater under intermittent supply of CO₂

Influence of light intensity on microalgal growth, nutrients removal and capture of carbon in the wastewater under intermittent supply of CO₂