Strong inbreeding depression and local outbreeding depression in the rewarding orchid Gymnadenia conopsea

Sletvold, Nina; Grindeland, John Magne; Zu, Pengjuan; Ågren, Jon

doi:10.1007/s10592-012-0373-7

Cited by 53 publications

(62 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Furthermore, the apparent reduced levels of selfing at DN may explain why the seeds from this population often outperformed those from other provenances regardless of where they were grown. Due to genetic purge, inbreeding depression is supposed to have less impact in selfers than in obligated outcrossers (Crnokrak and Barret, 2002) but several studies have reported larger seed production and greater seed performance for plants reproduced by outcrossing rather than selfing (Bellusci et al, 2009;Sletvold et al, 2012). The reasons of why plants at this site may behave differently are difficult to grasp and we are left to speculate.…”

Section: Discussionmentioning

confidence: 96%

A multi-faceted approach for assessing evolutionary significant conservation units in the endangered Omphalodes littoralis subsp. gallaecica (Boraginaceae)

López¹,

Retuerto²,

Roiloa³

et al. 2015

Perspectives in Plant Ecology, Evolution and Systematics

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 96%

A multi-faceted approach for assessing evolutionary significant conservation units in the endangered Omphalodes littoralis subsp. gallaecica (Boraginaceae)

López¹,

Retuerto²,

Roiloa³

et al. 2015

Perspectives in Plant Ecology, Evolution and Systematics

View full text Add to dashboard Cite

“…In this way pollinia can contact a stigma only for a short while after they have been removed from the donating flower. However, pollinia of G. conopsea do not show bending (Meekers et al 2012;Sletvold et al 2012b) and are therefore immediately susceptible to self-pollination.…”

Section: Discussionmentioning

confidence: 97%

“…Despite the increased fruiting success, fruits were more likely to originate from self-pollination, as the genetic profiles of seeds and mother plants were more often identical, indicating increased selfing. These higher selfing rates were reflected in a lower seed set possibly indicating inbreeding depression (Meekers and Honnay 2011;Sletvold et al 2012b). To avoid self-pollination during pollinator visits, some orchid species show delayed bending of pollinia (Peter and Johnson 2006).…”

Section: Discussionmentioning

confidence: 99%

“…In contrast, individuals within populations with thorough pollination and high fruiting success, such as those we studied, can suffer from a strong fitness decline due to increased selfing, while fruiting success hardly increases. In these populations, the advantages of an increase in pollen removal and deposition following a mutation for higher nectar AAs concentration would unlikely outweigh the negative consequences of increased self-pollination, especially since G. conopsea is highly susceptible to inbreeding depression through a strongly reduced seed production per fruit and lower germination rates (Sletvold et al 2012b). These limited benefits of increased nectar attraction might also explain the absence of phenotypic selection on G. conopsea nectar AA concentrations (Gijbels et al 2015).…”

Section: Discussionmentioning

confidence: 99%

“…Due to their minute seed size, mycorrhizal dependence and slow growth rates, estimating reproductive success of orchids is notoriously difficult (Sletvold et al 2012b). Therefore most studies estimate fitness and inbreeding depression in orchids as fruit and seed set (the proportion of seeds with embryos).…”

Section: Seed Set and Dna Analysesmentioning

confidence: 99%

See 2 more Smart Citations

Experimental fertilization increases amino acid content in floral nectar, fruit set and degree of selfing in the orchid Gymnadenia conopsea

et al. 2015

View full text Add to dashboard Cite

Floral traits have evolved to maximize reproductive success by attracting pollinators and facilitating pollination. Highly attractive floral traits may, however, also increase the degree of self-pollination, which could become detrimental for plant fitness through inbreeding depression. Floral nectar is a trait that is known to strongly mediate pollinator attraction and plant reproductive success, but the particular role of the nectar amino acid (AA) composition is poorly understood. Therefore, we experimentally manipulated the nectar AA composition and abundance of the Lepidoptera-pollinated orchid Gymnadenia conopsea through soil fertilization, and we quantified AA content and AA composition through high performance anion exchange chromatography with pulsed amperometric detection. Mixed models were then used to evaluate differences in pollinia removal, fruit set, seed set and degree of selfing between fertilized and control individuals. Selfing rates were estimated using microsatellite markers. We found that fertilized individuals had a significantly higher nectar AA content and an altered AA composition, whereas plant height, number of flowers, nectar volume and sugar concentration remained unchanged. Fertilized individuals also had significantly more pollinia removed and a higher fruit set, whereas control plants that did not receive the fertilization treatment had significantly fewer selfed seeds, and more viable seeds. Although we cannot exclude a role of changes in floral scent following the fertilization treatment, our results strongly suggest a relation among nectar AA composition, fruiting success and selfing rates. Our results also indicate potential consequences of nutrient pollution for plant reproductive success, through the induced changes in nectar AA composition.

show abstract

Fine‐scale genetic structure in the orchid Gymnadenia conopsea is not associated with local density of flowering plants

Sletvold,

Joffard,

Söderquist

2024

American J of Botany

Self Cite

View full text Add to dashboard Cite

PremiseDensity‐dependent pollinator visitation can lead to density‐dependent mating patterns and within‐population genetic structure. In Gymnadenia conopsea, individuals in low‐density patches receive more self pollen than individuals in high‐density patches, suggesting higher relatedness at low density. Ongoing fragmentation is also expected to cause more local matings, potentially leading to biparental inbreeding depression.MethodsTo evaluate whether relatedness decreases with local density, we analyzed 1315 SNP loci in 113 individuals within two large populations. We quantified within‐population genetic structure in one of the populations, recorded potential habitat barriers, and visualized gene flow using estimated effective migration surfaces (EEMS). We further estimated the magnitude of biparental inbreeding depression that would result from matings restricted to within 5 m.ResultsThere was no significant relationship between local density and relatedness in any population. We detected significant fine‐scale genetic structure consistent with isolation by distance, with positive kinship coefficients at distances below 10 m. Kinship coefficients were low, and predicted biparental inbreeding depression resulting from matings within the closest 5 m was a modest 1–3%. The EEMS suggested that rocks and bushes may act as barriers to gene flow within a population.ConclusionsThe results suggest that increased self‐pollen deposition in sparse patches does not necessarily cause higher selfing rates or that inbreeding depression results in low establishment success of inbred individuals. The modest relatedness suggests that biparental inbreeding depression is unlikely to be an immediate problem following fragmentation of large populations. The results further indicate that habitat structure may contribute to governing fine‐scale genetic structure in G. conopsea.

show abstract

Strong inbreeding depression and local outbreeding depression in the rewarding orchid Gymnadenia conopsea

Cited by 53 publications

References 65 publications

A multi-faceted approach for assessing evolutionary significant conservation units in the endangered Omphalodes littoralis subsp. gallaecica (Boraginaceae)

A multi-faceted approach for assessing evolutionary significant conservation units in the endangered Omphalodes littoralis subsp. gallaecica (Boraginaceae)

Experimental fertilization increases amino acid content in floral nectar, fruit set and degree of selfing in the orchid Gymnadenia conopsea

Fine‐scale genetic structure in the orchid Gymnadenia conopsea is not associated with local density of flowering plants

Contact Info

Product

Resources

About