Strong cation-exchange chromatography of proteins on a sulfoalkylated monolithic cryogel

Perçin, Işık; Khalaf, Rushd; Brand, Bastian; Morbidelli, Massimo; Gezici, Orhan

doi:10.1016/j.chroma.2015.01.075

Cited by 30 publications

(24 citation statements)

References 27 publications

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“…A sharp peak was observed in at the 1646 cm −1 wavelength (Figure A), characteristic of the amide grouping from the monomers (AAm and BAAm) used in cryogel synthesis . Perçin et al . and Gonçalves et al ., working with monolithic cryogels, identified the occurrence of strong bands around this same wavelength FTIR spectra, also associated with the presence of the amide group.…”

Section: Resultsmentioning

confidence: 91%

Enhancements in sugar immobilization in polymeric macroporous matrices for affinity capture

Silva

Nascimento

et al. 2019

J of Applied Polymer Sci

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The use of macroporous monolithic matrices in the purification of biocompounds is constantly growing and developing. In this work, the objective was to optimize the quantity of N-acetyl-D-glucosamine (D-GlcNAc) immobilized on the surface of macroporous polymeric cryogels for capture of lectins from less clarified solutions. Surface response methodology was applied and it was observed that the immobilization temperature of the glutaraldehyde (GLU) and the D-GlcNAc concentration influenced the amount of sugar immobilized. The matrices produced with 1.1% of allyl glycidyl ether were functionalized by GLU. Optimal maximum condition was obtained with mean value of 160.39 AE 26.38 mg of D-GlcNAc immobilized per gram of dry cryogel. Characterization analyses of the matrices showed that the activation process was effective, maintaining the macroporous structure and physical characteristics. The adsorbents produced were tested for capture of lectins from a crude protein solution of barley. At tested conditions, adsorbent capture around 11% of protein in solution but reduce the hemaglutinating capacity in 40%, demonstrating its selectivity. The cryogels functionalized with D-GlcNAc present potential for use in capture compounds by affinity with carbohydrates, such as lectins.

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Section: Resultsmentioning

confidence: 91%

Enhancements in sugar immobilization in polymeric macroporous matrices for affinity capture

Silva

Nascimento

et al. 2019

J of Applied Polymer Sci

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“…Moreover, silica does not swells or shrink in water and organic solvents and thus it offers a good environment as a support. Despite the mentioned desirable properties, poor chemical (hydrolytic) stability of silica is considered as an important problem that should be taken into account when working at highly acidic (pH \3) and alkaline (pH [8) conditions [23,24]. In other words, when it comes into contact with highly acidic and basic aqueous solutions, silica and silica-derived materials can hydrolyze and thus the ligand immobilized on the silica is loosen.…”

Section: Immobilization Of Calixarenesmentioning

confidence: 98%

“…Separation processes are being widely employed in diverse applications ranging from wastewater treatment to purification of drugs. The cost associated with these processes sometimes becomes very high as in the case of biopharmaceuticals [24]. For this Fig.…”

Section: Calixarene-based Sorbents and Hplc Stationary Phasesmentioning

confidence: 99%

Calixarene-engineered surfaces and separation science

Gezici

Bayrakcı

2015

J Incl Phenom Macrocycl Chem

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After their discovery, calixarenes have attracted the attention of many researchers from various disciplines. This group of supramolecules has an increasing popularity and this is most probably related with the flexibility of calixarene chemistry. Owing to their multifunctional character and stability, calixarenes became important precursors in separation science to derive new-type of sorbents or stationary phases. Immobilization of calixarenes to a suitable solid support (e.g. silica, synthetic polymers, magnetite nanoparticles, etc.) is a very popular concept being used for this purpose, and various immobilization methodologies have been proposed in the literature. In the present work, some state-of-the-art researches and developments published in the past are reviewed in a collective manner, and thus fundamentals of calixareneimmobilization and the application of the obtained materials in sorption and high performance liquid chromatography are represented.

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“…The good separation performance of monolithic capillary columns with gradient elution has been demonstrated for complex mixtures of proteins [135, 146–148]. Detobel et al [146], studied the effect of column parameters (morphology and length) and gradient conditions on the performance of capillary poly(styrene-co-divinylbenzene) monoliths.…”

Section: Different Stationary Phase Morphologies For Intact Protein Smentioning

confidence: 99%

“…Recently, the use of strong cation exchange (SCX) sulfoalkylated monolithic cryogel for the separation of 3 model proteins was reported [135]. The continuous network comprising interconnected macropores (10–100 μm) gave little or no mass-transfer resistance allowing the use of high flow rates without losing separation power.…”

Section: Different Stationary Phase Morphologies For Intact Protein Smentioning

confidence: 99%

Different Stationary Phase Selectivities and Morphologies for Intact Protein Separations

2016

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The central dogma of biology proposed that one gene encodes for one protein. We now know that this does not reflect reality. The human body has approximately 20,000 protein-encoding genes; each of these genes can encode more than one protein. Proteins expressed from a single gene can vary in terms of their post-translational modifications, which often regulate their function within the body. Understanding the proteins within our bodies is a key step in understanding the cause, and perhaps the solution, to disease. This is one of the application areas of proteomics, which is defined as the study of all proteins expressed within an organism at a given point in time. The human proteome is incredibly complex. The complexity of biological samples requires a combination of technologies to achieve high resolution and high sensitivity analysis. Despite the significant advances in mass spectrometry, separation techniques are still essential in this field. Liquid chromatography is an indispensable tool by which low-abundant proteins in complex samples can be enriched and separated. However, advances in chromatography are not as readily adapted in proteomics compared to advances in mass spectrometry. Biologists in this field still favour reversed-phase chromatography with fully porous particles. The purpose of this review is to highlight alternative selectivities and stationary phase morphologies that show potential for application in top-down proteomics; the study of intact proteins.

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Strong cation-exchange chromatography of proteins on a sulfoalkylated monolithic cryogel

Cited by 30 publications

References 27 publications

Enhancements in sugar immobilization in polymeric macroporous matrices for affinity capture

Enhancements in sugar immobilization in polymeric macroporous matrices for affinity capture

Calixarene-engineered surfaces and separation science

Different Stationary Phase Selectivities and Morphologies for Intact Protein Separations

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