Strong bottlenecks, inbreeding and multiple hybridization of threatened European Ruppia maritima populations

Triest, Ludwig; Sierens, Tim

doi:10.1016/j.aquabot.2015.05.001

Cited by 17 publications

(55 citation statements)

References 51 publications

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“…Heterogeneous PhyB sequences (Ito et al, , ) or ITS sequences (Martinez‐Garrido et al, ) and a duplicated microsatellite marker RC3 (Triest & Sierens, ) were used as potential indicators of tetraploidy at individual level. So far, 12 microsatellite markers have been developed for European R. maritima and related hybrids (Triest & Sierens, ). Six microsatellite loci were specifically developed for R. cirrhosa from the Southern Iberian peninsula and confirmed similar entities and hybridization events in a regional study (Martinez‐Garrido et al, ) than reported across Europe in Triest and Sierens ().…”

Section: Methodsmentioning

confidence: 99%

“…To enhance the understanding of the phylogenetic relationships within the genus, chloroplast markers and a limited set of nuclear markers were used to study different Ruppia species and populations from different continents (Ito, Ohi‐Toma, Murata, & Tanaka, , ; Martinez‐Garrido, Serrao, Engelen, & González‐Wangüemert, ; Triest & Sierens, , , , ; Yu, Shi, & Chen, ). These markers revealed independently a high level of complexity within the genus, caused by hybridization, introgression, polyploidy, and haplotype convergence.…”

Section: Introductionmentioning

confidence: 99%

“…Only three ITS variants of Ruppia were detected so far and should be complemented with additional nuclear markers to unravel delineation of Ruppia taxa (Triest & Sierens, ). Individuals belonging to R. maritima and R. drepanensis were considered diploid, whereas tetraploidy was observed in most other taxa (Martinez‐Garrido et al, ; Triest & Sierens, , ). When used in ecological studies, populations containing different ploidy levels or cryptic species could lead to inaccurate estimations of, for example, annual versus perennial survival strategies or biomass allocation in belowground and aboveground structures (Mannino et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…An out‐of‐Africa hypothesis was presented for many Mediterranean lineages (Triest & Sierens, ) and the W Mediterranean can be considered as the origin of R. cirrhosa diversity (Martinez‐Garrido et al, ; Triest & Sierens, , ). A higher level of haplotypic diversity in Ruppia populations was reported for the Iberian peninsula than in the Eastern Mediterranean basin (Triest & Sierens, ) and 12 microsatellite markers developed for R. maritima aided in identification of ancient introgressed and recent hybrids with R. cirrhosa (Triest & Sierens, ). Six microsatellite loci were specifically developed for R. cirrhosa from the Southern Iberian peninsula and confirmed similar entities and hybridization events in a regional study (Martinez‐Garrido et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…Sequences of Ruppia deposited in GenBank indicated an overall low resolution of taxonomic entities because of too short partial sequences in rbcL (Martinez‐Garrido et al, ; Triest & Sierens, ) and an overall shortage of parsimonious sites. A widely used intergenic spacer region ( trnH‐psbA ) revealed phenetic entities with strong support (Triest & Sierens, , ), Standard phylogenetic analyses ignore information from insertion–deletion (indel) variations, thus underestimating diversity. Indels might have potential for better resolution in barcoding without leading to drastic differences if indels are not considered (Raymundez, Mathez, Xena de Enrech, & Dubuisson, ).…”

Section: Introductionmentioning

confidence: 99%

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Lagoons and saltwater wetlands getting more diversity: A molecular approach reveals cryptic lineages of a euryhaline submerged macrophyte (Ruppia)

Triest

Beirinckx

Sierens

2017

Aquatic Conservation

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Ruppia species are distantly related to seagrasses and occur in saltwater coastal and inland lagoons, mostly as monospecific beds. The diversity of euryhaline Ruppia populations from different continents recently became better understood from a suite of chloroplast sequences but limited nuclear markers. These revealed a high complexity from hybridization, introgression, polyploidy and haplotypic divergence. Because of this complexity within the genus and multiple allelic states in polyploids, three multiplexed sets of 24 nuclear microsatellites were developed from four Ruppia provenances and cross‐amplified on 130 individuals from a wide range of taxa in various aquatic habitats on different continents. Secondly, pure R. cirrhosa and R. maritima individuals could be unambiguously identified from their introgressed hybrids and from other known or yet unidentified taxa, using diagnostic markers that referred to autotetraploid individuals in R. cirrhosa and allotetraploidy in an ancient hybrid complex ‘haplogroup E’. Thirdly, a phenetic barcoding approach of trnH‐psbA chloroplast haplotypes taking into account insertion–deletion variations, revealed lineages of recently described taxa from lagoons in different continents (i.e. R. sinensis, R. brevipedunculata, R. mexicana) in addition to separate lineages of hybrid origin. Congruence between pollination mode and diversification of lineages, allows one to hypothesize whether selfing underwater leads to clearly separated lineages whereas outcrossing at the water surface allows hybridization and extensive introgression with potential chloroplast capture. This study raises a renewed interest in cryptic lineages, hybrid taxa and shallow phylogenies of Ruppia lineages, thereby critically questioning worldwide distributions of least concern species. Recognition and monitoring of unique Ruppia lineages will support further studies on connectivity, survival strategies and movement ecology to aid in determining the conservation status of a wide variety of lagoon and coastal wetland habitats.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Lagoons and saltwater wetlands getting more diversity: A molecular approach reveals cryptic lineages of a euryhaline submerged macrophyte (Ruppia)

Triest

Beirinckx

Sierens

2017

Aquatic Conservation

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Reproductive ecology and postpollination development in the hydrophilous monocot Ruppia maritima

Taylor

Giffei

Dang

et al. 2020

American J of Botany

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Premise Water‐pollination (hydrophily) is a rare but important pollination mechanism that has allowed angiosperms to colonize marine and aquatic habitats. Hydrophilous plants face unique reproductive challenges, and many have evolved characteristic pollen traits and pollination strategies that may have downstream consequences for pollen performance. However, little is known about reproductive development in the life history stage between pollination and fertilization (the progamic phase) in hydrophilous plants. The purpose of this study was to characterize reproductive ecology and postpollination development in water‐pollinated Ruppia maritima L. Methods Naturally pollinated inflorescences of R. maritima were collected from the field. Experimental pollinations using both putatively outcross and self pollen were conducted in the greenhouse and inflorescences were collected at appropriate intervals after pollination. Pollen reception, pollen germination, pollen tube growth, and carpel morphology were characterized. Results Ruppia maritima exhibits incomplete protogyny, allowing for delayed selfing. Pollen germinated within 15 min after pollination. The average shortest possible pollen tube pathway was 425 μm and pollen tubes first reached the ovule at 45 min after pollination. The mean adjusted pollen tube growth rate was 551 μm/h. Conclusions Ruppia pollen is adapted for rapid pollen germination, which is likely advantageous in an aquatic habitat. Small effective pollen loads suggest that pollen competition intensity is low. Selection for traits such as a long period of stigma receptivity, fast pollen germination, and carpel morphology likely played a larger role in shaping postpollination reproductive development in Ruppia than evolution in pollen tube growth rates.

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Regional Genetic Structure in the Aquatic Macrophyte Ruppia cirrhosa Suggests Dispersal by Waterbirds

Martínez-Garrido

Bermejo

Serrão

et al. 2017

Estuaries and Coasts

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The evolutionary history of the genus Ruppia has been shaped by hybridization, polyploidisation and vicariance, that have resulted in a problematic taxonomy. Recent studies provided insight into species circumscription, organelle takeover by hybridization, and revealed the importance of verifying species identification to avoid distorting effects of mixing different species, when estimating population connectivity. In the present study, we use microsatellite markers to determine population diversity and connectivity patterns in Ruppia cirrhosa including two spatial scales: 1) from the Atlantic Iberian coastline in Portugal to the Siculo-Tunisian strait in Sicily, and 2) within the Iberian peninsula comprising the Atlantic-Mediterranean transition. The higher diversity in the Mediterranean Sea suggests that populations have had longer persistence there, suggesting a possible origin and/or refugial area for the species. The high genotypic diversities highlight the importance of sexual reproduction for survival and maintenance of populations.Results revealed a regional population structure matching a continent-island model, with strong genetic isolation and low gene flow between populations. This population structure could be maintained by waterbirds, acting as occasional dispersal vectors. This information elucidates ecological strategies of brackish plants species in coastal lagoons, suggesting mechanisms used by this species to colonize new isolated habitats and dominate brackish aquatic macrophyte systems, yet maintaining strong genetic structure suggestive of very low dispersal.

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Strong bottlenecks, inbreeding and multiple hybridization of threatened European Ruppia maritima populations

Cited by 17 publications

References 51 publications

Lagoons and saltwater wetlands getting more diversity: A molecular approach reveals cryptic lineages of a euryhaline submerged macrophyte (Ruppia)

Lagoons and saltwater wetlands getting more diversity: A molecular approach reveals cryptic lineages of a euryhaline submerged macrophyte (Ruppia)

Reproductive ecology and postpollination development in the hydrophilous monocot Ruppia maritima

Regional Genetic Structure in the Aquatic Macrophyte Ruppia cirrhosa Suggests Dispersal by Waterbirds

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