Strong Antibacterial Polydopamine Coatings Prepared by a Shaking-assisted Method

Abstract

Strong antibacterial polydopamine (PDA) coatings prepared by a facile shaking-assisted method is reported for the first time. It was found that a minor modification made to the conventional synthesis procedure of PDA coatings, viz. replacing the static solution condition with a shaking solution condition by using a mechanical shaker, can produce the roughened polydopamine (rPDA) coatings at different substrates, e.g., glass, stainless steel, plastic, and gauze. The resulting rPDA coatings were characterized with Raman spectrum, zeta-potential analysis and contact angle measurement. The antibacterial activity of the rPDA coatings was evaluated by a shake flask test with gram-positive Staphylococcus aureus, and gram-negative Escherichia coli and Pseudomonas aeruginosa as bacteria models. Testing results revealed that, in the absence of any other antibacterial agents, the rPDA coatings exhibited remarkably enhanced antibacterial activities. In addition, such enhanced antibacterial activities of the rPDA coatings were found to be unimpaired by steam sterilization treatments.

Polydopamine functionalized hydrogel beads as magnetically separable antibacterial materials

“…4(c2 and c3). Our obtained FE-SEM ndings of PDA coatings were similar to rough PDA (rPDA) coated glass substrates synthesized by Su et al 38 For a clear view of the internal structure, the freeze-dried beads were cross-sectioned using a sharp blade and then viewed. Fig.…”

“…These ndings are in consistence with previous studies where PDA is known to make surfaces more hydrophilic. 38,59,60 Elasticity and viscosity are signicant properties related to hydrogel materials. Any change in the structure will reect a change in these properties.…”

“…Versatile adhesion capability, catalytic, antimicrobial and antifouling properties of PDA renders it as a suitable functionalization material. [33][34][35][36][37][38] Also, like other natural adhesive proteins PDA exhibits good biocompatibility and low-toxicity to the environment. 39 Hence, use of PDA as a functionalization material can serve as a facile and ecofriendly strategy to provide stability and antibacterial competence.…”

Polydopamine‐collagen complex to enhance the biocompatibility of polydimethylsiloxane substrates for sustaining long‐term culture of L929 fibroblasts and tendon stem cells

“…Alternatively, surface coating with polydopamine (PDA) has been proved as an effective method for supporting cell adhesion and long‐term proliferation on PDMS . Dopamine (DA) is generally considered as a type of cell‐compatible molecules, which can be strongly adsorbed onto a majority of substrate materials through covalent conjugation or physical interactions . However, only a few reports demonstrated that PDA can be codeposited with other functional molecules onto PDMS surfaces to achieve stabilized adhesion and biological activity.…”

Probing polydopamine adhesion to protein and polymer films: microscopic and spectroscopic evaluation

“…The mussel adhesive-inspired polydopamine (PD) appears to be an ideal biomaterial coating since it fulfils all these criteria. It exhibits low cytotoxicity [2], antibacterial properties [3,4], promotes cell growth [5][6][7][8], resists corrosion [9], can aid antibiotic release [10,11], is simple to produce with control over film thickness [12] and can be attached to a variety of surfaces including silanes [13], metals [9,14], polymers [7,[15][16][17] dentine [18] and even hair [19].…”