Stromelysin-3 over-expression enhances tumourigenesis in MCF-7 and MDA-MB-231 breast cancer cell lines: involvement of the IGF-1 signalling pathway

Kasper, Grit; Reule, Matthias; Tschirschmann, Miriam; Dankert, Niels; Stout-Weider, Karen; Lauster, Roland; Schröck, Evelin; Mennerich, Detlev; Duda, Georg N.; Lehmann, Kerstin

doi:10.1186/1471-2407-7-12

Cited by 43 publications

(33 citation statements)

References 37 publications

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“…For this we used the BR55 MB targeted against the human VEGFR2 which different from most previous approaches [11,12,[14][15][16][17]22]. Most importantly, the coupling of VEGFR2-targeting ligands to the MB was not performed via the potentially immunogenic streptavidin-biotin method.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, the purpose of this study was to investigate the circulation characteristics of novel nonspecific microbubbles (BR38) and of VEGFR2-targeted (BR55) microbubbles (MB), both suited to clinical use. Furthermore, their ability to discriminate differences in the angiogenesis of oestrogenindependent, highly aggressive (MDA-MB-231) and oestrogen-dependent, low aggressive (MCF-7) breast cancer xenografts [20][21][22], respectively, was evaluated. With BR38 we assessed the vascularization as an angiogenic marker and with BR55 the VEGFR2-status.…”

Section: Introductionmentioning

confidence: 99%

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Molecular and functional ultrasound imaging in differently aggressive breast cancer xenografts using two novel ultrasound contrast agents (BR55 and BR38)

et al. 2011

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular and functional ultrasound imaging in differently aggressive breast cancer xenografts using two novel ultrasound contrast agents (BR55 and BR38)

et al. 2011

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“…In addition, anthocyanin intake inhibits tumor proliferation in patients with colorectal cancer (6,10). Studies have also indicated that various anthocyanins derived from fruits and vegetables are effective in the prevention or reduction of solid tumor development in animal models (11)(12)(13). Altogether, these data indicate that using dietary chemopreventive ONCOLOGY REPORTS 24: 1471-1477 Anthocyanins are novel AMPK·1 stimulators that suppress tumor growth by inhibiting mTOR phosphorylation agents, such as AIMs, might be a promising strategy for controlling colon cancer.…”

Section: Introductionmentioning

confidence: 89%

Anthocyanins are novel AMPKα1 stimulators that suppress tumor growth by inhibiting mTOR phosphorylation

Lee

Lee²,

Kim³

et al. 2010

Oncol Rep

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Abstract. AMP-activated protein kinase (AMPK) has emerged as a therapeutic target of cancer. AMPK functions as an upstream regulator of proliferative signals such as mammalian target of rapamycin (mTOR), tuberous sclerosis complex (TSC), p70S6 and elongation factor-2, indicating that AMPK can be applied for the inhibition of cancer cell proliferation via modulating the proliferative signaling network. The Akt/mTOR signaling pathway is activated in colon cancer. The well known mTOR inhibitor rapamycin has a disadvantage of feedback stimulation of Akt. Anthocyanins are naturally-occurring mTOR inhibitor possessing Akt inhibitory activities. We have investigated the mTOR inhibitory effect of anthocyanins through the activation of AMPK. In this study, anthocyanins were applied to colon cancer cells and tumor-bearing xenograft models to investigate their anti-proliferative and pro-apoptotic effects, and elucidate the mechanisms that link AMP-activated protein kinase (AMPK) ·1 activation to the survival signal of mTOR. Our results indicated that anthocyanins significantly decreased phospho-mTOR comparable to rapamycin, a synthetic mTOR inhibitor, and this inhibitory effect of anthocyanins on mTOR was completely abrogated by inactivating AMPK·1. Furthermore, suppression of cell growth with anthocyanins was also alleviated in the absence of noticeable AMPK·1 activities. For the first time we have found anthocyanins as novel AMPK·1 activators, and in conditions of AMPK·1 inactivation, anthocyanins lost their ability to inhibit mTOR in HT-29 colon cancer cells. The activation of AMPK·1, and the deactivation of mTOR and Akt were observed in anthocyanins-treated tumor-bearing xenograft models. The results from this study suggest that there is a complex interaction between AMPK·1 and mTOR signaling, and anthocyanins are powerful AMPK·1 activators that inhibit cancer cell growth by inhibiting mTOR phosphorylation.

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“…IGF-I signaling can promote the tumor growth and invasion through regulation of PCNA and VEGF expression (33,34). Furthermore, MMP II leads to the stimulation ofthe IGF-I R pathway in carcinoma cells, thus enhancing their proliferative capacity (30,35). In the present study, our finding showed that knockdown ofMMP II downregulated the expression ofIGF-I, PCNA and VEGF and suppressed the bioactivities in GAC cells, suggesting that MMPII may be involved in the development and progression of gastric cancer via IGF-I-mediated regulation of the PCNA and VEGF expression.In conclusion, our findings demonstrate that MMP II expression is increased in human GAC tissues, but does not correlate with the clinicopathologic features.…”

Section: Discussionmentioning

confidence: 99%

Knockdown of MMP11 Inhibits Proliferation and Invasion of Gastric Cancer Cells

et al. 2013

Int J Immunopathol Pharmacol

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Matrix metalloproteinase 11(MMPll or stromelysin-3) has recently been reported to playa crucial role in the development and progression of multiple malignancies. The aim of this study was to investigate the function ofMMPll expression in human gastric adenocarcinoma (GAC). Using immunohistochemistry assay, we studied the expression level of MMPll in GAC and adjacent non-cancerous tissues (ANCT). The association between MMPll expression and tumor size and pathological grade, as well as metastatic potential was analyzed. Through small hairpin RNA (shRNA)-mediated MMPll knockdown in SGC-7901 GAC cells, we observed the changes of the biological behaviors of GAC cells. Our results indicated that the rate of positive expression ofMMPll was higher in GAC tissues than in ANCT (55.0% vs 30.0%, P=0.025). MMPll expression had no association with the factors of age or gender of the GAC patients, or the size, pathological staging and lymph node metastases of the tumors (each P>0.05). Furthermore, MMPll knockdown inhibited the proliferative activities and invasive potential of SGC-7901 GAC cells with decreased expression of IGF-l, PCNA and VEGF. Taken together, our findings demonstrated that MMPll expression was increased in GAC tissues, but did not correlate with the clinicopathologic features. Knockdown of MMPll expression could inhibit the proliferation and invasion of GAC cells probably through down-regulation of the IGF-l signaling pathway, suggesting that MMPll might be a potential therapeutic target for the treatment of gastric cancer.Gastric cancer (GC) is a world health burden, ranging as the second cause of cancer death worldwide. The prognosis of gastric cancer is poor with an estimated relative five year survival rate of less than 20% (1). Etiologically, GC arises not only from the combined effects of environmental factors and susceptible genetic variants but also from the accumulation of genetic and epigenetic alterations. Molecular oncobiology studies have brought to light a number of genes that are implicated in gastric carcinogenesis (2).Matrix metalloproteases (MMPs) playa key role in the metabolism of connective tissue proteins in the norm and in pathology. Major MMP subfamilies and matrixins act through promoting the oncogenic transformation of the cells, and angiogenesis (3). MMPs constitute one of the major extracellular matrix degrading enzymic families implicated in cancer development. MMP11 in particular, a member of the matrix metalloproteinases, exhibits collagenolytic function against collagen VI under normal and malignant conditions (4), and is closely related to invasiveness and tumor progression (5). MMP-ll expression is simulated in mouse

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Stromelysin-3 over-expression enhances tumourigenesis in MCF-7 and MDA-MB-231 breast cancer cell lines: involvement of the IGF-1 signalling pathway

Cited by 43 publications

References 37 publications

Molecular and functional ultrasound imaging in differently aggressive breast cancer xenografts using two novel ultrasound contrast agents (BR55 and BR38)

Molecular and functional ultrasound imaging in differently aggressive breast cancer xenografts using two novel ultrasound contrast agents (BR55 and BR38)

Anthocyanins are novel AMPKα1 stimulators that suppress tumor growth by inhibiting mTOR phosphorylation

Knockdown of MMP11 Inhibits Proliferation and Invasion of Gastric Cancer Cells

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