Stromal Vascular Fraction Reverses the Age-Related Impairment in Revascularization following Injury

Abstract

Adipose-derived stromal vascular fraction (SVF) has emerged as a potential regenerative therapy, but few studies utilize SVF in a setting of advanced age. Additionally, the specific cell population in SVF providing therapeutic benefit is unknown. We hypothesized that aging would alter the composition of cell populations present in SVF and its ability to promote angiogenesis following injury, a mechanism that is T cell-mediated. SVF isolated from young and old Fischer 344 rats was examined with flow cytometry for cell composition. Mesenteric windows from old rats were isolated following exteriorization-induced (EI) hypoxic injury and intravenous injection of one of four cell therapies: (1) SVF from young or (2) old donors, (3) SVF from old donors depleted of or (4) enriched for T cells. Advancing age increased the SVF T-cell population but reduced revascularization following injury. Both young and aged SVF incorporated throughout the host mesenteric microvessels, but only young SVF significantly increased vascular area following EI. This study highlights the effect of donor age on SVF angiogenic efficacy and demonstrates how the ex vivo mesenteric-window model can be used in conjunction with SVF therapy to investigate its contribution to angiogenesis.

Analyzing the Clinical Potential of Stromal Vascular Fraction: A Comprehensive Literature Review

“…After removing duplicates and screening titles and abstracts, 84 full-text articles were assessed for eligibility. Of these, 16 articles met the inclusion criteria and were included in the review (Table 1) [11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26]. The selected studies included prospective and/or retrospective case series, randomized controlled clinical trials, and reviews.…”

“…The study demonstrated that tissue-engineered dermis grafts incorporating SVF yielded superior scar quality in the alar zone of the nose compared to traditional artificial dermis grafts. This finding is significant as it highlights SVF's potential in improving aesthetic outcomes in reconstructive surgery, offering more effective solutions for challenging areas like nasal defects [19,21]. Jeon et al [24] reported increased fat graft survival rates in breast reconstruction with SVF.…”