Stromal p16 Overexpression in Adult Granulosa Cell Tumors of the Ovary

Abstract

Our observations suggest that stromal p16 expression may be involved in the development and progression of ovarian adult granulosa cell tumors.

TP53 Mutation Status of Tubo-ovarian and Peritoneal High-grade Serous Carcinoma with a Wild-type p53 Immunostaining Pattern

“…These slices were deparaffinized in xylenes and rehydrated through graded alcohols. Immunohistochemical staining was performed using automatic immunostaining instruments, either a Ventana Benchmark XT automated staining system (Ventana Medical Systems) or Dako Omnis system (Dako, Agilent Technologies, Carpinteria, CA, USA), according to the manufacturer's recommendations (13)(14)(15)(16)(17). Antigen retrieval was performed using Cell Conditioning Solution (CC1; Ventana Medical Systems) or EnVision FLEX Target Retrieval Solution, High pH (Dako, Agilent Technologies).…”

The role of p16 as putative biomarker for cervical neoplasia: A controversial issue?

“…23 or hypermethylation; 5,6 this is the a reason why The Lower Anogenital Squamous Terminology Standardization Project for HPV-associated Lesions (LAST Project) suggested p16 staining as a preferred biomarker for cervical lesions. 7 HPV oncogenic types associated with high grade lesions are characterized by overexpression of p16, which can be detected by immunohistochemistry.…”

Predictive Value of Sphingosine Kinase 1 Expression in Papillary Thyroid Carcinoma

“…Immunohistochemical staining was performed using a compact polymer method (Bond Intense Detection Kit, Leica Biosystems, Newcastle upon Tyne, UK) (3,7,14,(26)(27)(28)(29)(30). The 4-μm-thick, formalin-fixed, paraffin-embedded slices were deparaffinized and dehydrated with xylene and then rehydrated in a graded series of alcohol solutions.…”