Stromal elastosis in papillary thyroid carcinomas

Kondo, Tetsuo; Nakazawa, Tadao; Murata, Shin‐ichi; Katoh, Ryohei

doi:10.1016/j.humpath.2005.03.006

Cited by 10 publications

(10 citation statements)

References 22 publications

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“…Second, elastin in normal stroma displays the morphology of strings, while elastin in neoplastic is shorter and more fragmental, and it also has an irregular getting together tendency and an increased amount, like stromal elastosis. 5 Last, as can be seen in the combined SHG/TPEF figures, there is a significant alteration in the proportions between collagen and elastic fibers, consistent with which the neoplastic stroma consists of abnormal proportions of matrix molecules. 2 In the following, we performed the quantitative analysis of these interesting results.…”

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confidence: 56%

Extracting diagnostic stromal organization features based on intrinsic two-photon excited fluorescence and second-harmonic generation signals

et al. 2009

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Abstract. Intrinsic two-photon excited fluorescence ͑TPEF͒ and second-harmonic generation ͑SHG͒ signals are shown to differentiate between normal and neoplastic human esophageal stroma. It was found that TPEF and SHG signals from normal and neoplastic stroma exhibit different organization features, providing quantitative information about the biomorphology and biochemistry of tissue. By comparing normal with neoplastic stroma, there were significant differences in collagen-related changes, elastinrelated changes, and alteration in proportions of matrix molecules, giving insight into the stromal changes associated with cancer progression and providing substantial potential to be applied in vivo to the clinical diagnosis of epithelial precancers and cancers. A number of optical spectroscopic and imaging techniques have widely been used for the in vivo, real-time detection of epithelial precancers and cancers.1 However, most studies focus only on the epithelium to analyze morphological, structural, and architectural changes that accompany development of epithelial precancers.2 Recently, it has been recognized that stromal biology is also altered significantly with various pathological processes.2 However, there is a lack of utilizing the alterations in the stroma as an intrinsic indicator of disease states, which can extract quantitative information about the biomorphology and biochemistry and can provide a new means to improve early detection of neoplastic changes.Multiphoton microscopy has several advantages over traditional confocal microscopy, providing high-resolution images at increased imaging depths, minimal out-of-plane absorption, and inherent optical sectioning.3 It is well suited for the observation of unstained samples based on intrinsic sources of nonlinear signals, 4 such as two-photon excited fluorescence ͑TPEF͒ and second-harmonic generation ͑SHG͒. It also has been used for in vivo cancer imaging. 3Our primary goals in this study are to investigate the intrinsic TPEF and SHG signals in epithelial stroma and to extract biomorphologic and biochemical features for potential tissue diagnosis.The multiphoton system used in this study has been described previously. 4 Briefly, it is an Axiovert 200 microscope ͑Zeiss LSM 510 META͒ equipped with a mode-locked femtosecond Ti:sapphire laser ͑Coherent Mira 900-F͒. The polarization of laser light is the linear polarization. To suppress TPEF signals from collagen, the excitation wavelength at 850 nm was used in this work. 4 An oil immersion objective ͑ϫ63 and NA= 1.4͒ was employed. The META detector was used to detect all signals in backscattered geometry. The average laser power at the sample was 5 mW. In this study, all images were 512ϫ 512 pixels. The images were obtained at 2.56 s per pixel. In this work, considering that the surface of the sections is not absolutely flat and that the signals in the surface of sections is not absolutely maximal, we defined a depth of 0 m, referring to the position where the multiphoton signals of reflection from the interfac...

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confidence: 56%

Extracting diagnostic stromal organization features based on intrinsic two-photon excited fluorescence and second-harmonic generation signals

et al. 2009

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“…Stromal elastosis, as indicated by dense aggregates of elastic fibers, is a pathological stromal alteration that is occasionally encountered in various neoplasms. Tumor‐related elastosis has been investigated in several human neoplasms, for example, in breast, lung and thyroid neoplasms 5–7 . However, little is known about this peculiar type of stromal response in neoplasms.…”

Section: Discussionmentioning

confidence: 99%

“…However, little is known about this peculiar type of stromal response in neoplasms. Some studies have suggested that there is an association between stromal elastosis and invasiveness, 5–9 and periductal elastosis commonly occurs in periductal carcinomas of the breast, particularly when invasive areas are present. In the present study, stromal elastosis was more prominent in BCC than in TE.…”

Section: Discussionmentioning

confidence: 99%

Elastic fiber staining and cytokeratin 15 expression pattern in trichoepithelioma and basal cell carcinoma

Choi

Park

Kim

et al. 2008

The Journal of Dermatology

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Trichoepithelioma (TE) is a benign neoplasm of the skin that resembles basal cell carcinoma (BCC) in its clinical and histological features. In this study, we evaluate the usefulness of elastic fiber staining and cytokeratin 15 expression pattern in terms of distinguishing TE from BCC. Eleven TE and 17 BCC were examined histochemically and immunohistochemically. It was found that BCC contain more elastic fiber than TE, and that more TE show peripheral localization than BCC in cytokeratin 15 expression patterns. The present study shows that elastic fiber staining and cytokeratin 15 expression pattern may aid the differentiation of TE from BCC.

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“…15,16 The weak immunoreactivity of fibrillin-1 in the stroma of thyroid tumors suggests a mechanism probably inhibiting the deposition of the protein in the tumorous stroma, since aberrant deposition of matrix proteins has been reported in many tumor types. This might be especially important for papillary carcinoma which exhibits a high degree of elastosis, 17 inasmuch as fibrillin-1 has been suggested to be colocalized with elastin in various tissues. 8,18 Although the mechanism of fibrillin-1 deposition in ECM is largely unknown, it has been shown that in epithelial cells, soluble heparin competitively inhibits an unknown interaction that is needed for ECM deposition of both fibrillin-1 and -2.…”

Section: Discussionmentioning

confidence: 99%

Fibrillin expression and localization in various types of carcinomas of the thyroid gland

et al. 2006

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Fibrillin is an extracellular matrix (ECM) glycoprotein, a main component of microfibrills, suggested to support cell attachment and to impact cell differentiation and migration. The aim of this study was to investigate fibrillin-1 expression in thyroid carcinomas at mRNA and protein level, since ECM proteins are suggested to be of great importance for the metastatic potential of carcinomas. RNA was extracted from 13 thyroid carcinoma cell lines and RT-PCR analysis with gene-specific primers revealed fibrillin-1 mRNA expression in all cell lines, with highest expression in the follicular carcinoma cell line WRO and lowest expression in the two anaplastic cell lines (APO, FRO). Furthermore, we investigated fibrillin-1 expression by immumohistochemistry in a commercially available tissue microarray including 50 thyroid carcinomas as well as in archival tissue from 33 thyroid carcinomas. Fibrillin-1 demonstrated a cytoplasmic location in the neoplastic cells of almost all carcinomas apart from the follicular ones. The most intense staining was observed in papillary carcinomas with some evidence of a slight increased intensity in advanced stages. Our data indicate that fibrillin-1 is strongly expressed by the neoplastic cells of thyroid carcinomas in different degree in the various histologic types and might be implicated in cell-stroma interaction in terms of signaling, attachment and migration.

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Stromal elastosis in papillary thyroid carcinomas

Cited by 10 publications

References 22 publications

Extracting diagnostic stromal organization features based on intrinsic two-photon excited fluorescence and second-harmonic generation signals

Extracting diagnostic stromal organization features based on intrinsic two-photon excited fluorescence and second-harmonic generation signals

Elastic fiber staining and cytokeratin 15 expression pattern in trichoepithelioma and basal cell carcinoma

Fibrillin expression and localization in various types of carcinomas of the thyroid gland

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