Stroke Therapy: the Potential of Amniotic Fluid-Derived Stem Cells

Elías, Maya N.; Hoover, Jaclyn; Nguyen, Hung; Reyes, Stephanny; Lawton, Christopher; Borlongan, Cesar V.

doi:10.2217/fnl.15.19

Cited by 11 publications

(13 citation statements)

References 42 publications

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“…The high proliferative capacity, the low immunogenicity, and tumorgenicity and the anti‐inflammatory activity support the hAFSCs use as safe and effective donor cells for stroke therapy 88 . Furthermore, human stem cell utilization is useful and helpful for clinical trial translation.…”

Section: Discussionmentioning

confidence: 99%

“…78,87 The high proliferative capacity, the low immunogenicity, and tumorgenicity and the anti-inflammatory activity support the hAFSCs use as safe and effective donor cells for stroke therapy. 88 Further, within exosomal cargo, a broad variety of miRNAs has been detected, which can influence functions associated with neural remodeling and neurogenic processes. Several miRNAs, already identified as players in neuroprotective mechanisms (miR-146a-5p, miR-154-5p, miR-22-3p, miR-23a-3p, miR-27a-3p, miR-29a-3p, and miR-31-5p), were also identified in our hAFSC-derived exosomes.…”

Section: Discussionmentioning

confidence: 99%

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Neuroprotective effects of human amniotic fluid stem cells-derived secretome in an ischemia/reperfusion model

Castelli

Antonucci

Tessitore

et al. 2020

Stem Cells Translational Medicine

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Stem cells offer the basis for the promotion of robust new therapeutic approaches for a variety of human disorders. There are still many limitations to be overcome before clinical therapeutic application, including a better understanding of the mechanism by which stem cell therapies may lead to enhanced recovery. In vitro investigations are necessary to dissect the mechanisms involved and to support the potential development in stem cell-based therapies. In spite of growing interest in human amniotic fluid stem cells, not much is known about the characteristics of their secretome and regarding the potential neuroprotective mechanism in different pathologies, including stroke. To get more insight on amniotic fluid cells therapeutic potential, signal transduction pathways activated by human amniotic fluid stem cells (hAFSCs)derived secretome in a stroke in vitro model (ischemia/reperfusion [I/R] model) were investigated by Western blot. Moreover, miRNA expression in the exosomal fraction of the conditioned medium was analyzed. hAFSCs-derived secretome was able to activate prosurvival and anti-apoptotic pathways. MicroRNA analysis in the exosomal component revealed a panel of 16 overexpressed miRNAs involved in the regulation of coherent signaling pathways. In particular, the pathways of relevance in ischemia/reperfusion, such as neurotrophin signaling, and those related to neuroprotection and neuronal cell death, were analyzed. The results obtained strongly point toward the neuroprotective effects of the hAFSCs-conditioned medium in the in vitro stroke model here analyzed. This can be achieved by the modulation and activation of pro-survival processes, at least in part, due to the activity of secreted miRNAs.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Neuroprotective effects of human amniotic fluid stem cells-derived secretome in an ischemia/reperfusion model

Castelli

Antonucci

Tessitore

et al. 2020

Stem Cells Translational Medicine

View full text Add to dashboard Cite

show abstract

“…Preclinical data of the safety and efficacy of AFSCs suggest that individuals who suffer a stroke and show significant inflammation of the brain, or who display short-term memory loss due to the accompanying injury to the hippocampus, may be ideal candidates for future clinical trials of AFSC transplantation 1,33 .…”

Section: Discussionmentioning

confidence: 99%

Intravenous Grafts of Human Amniotic Fluid-Derived Stem Cells Reduce Behavioral Deficits in Experimental Ischemic Stroke

et al. 2019

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Amniotic fluid has been investigated as new cell source for stem cells in the development of future cell-based transplantation. This study reports isolation of viable human amniotic fluid-derived stem cells, labeled with multimodal iron oxide nanoparticles, and its effect on focal cerebral ischemia–reperfusion injury in Wistar rats. Middle cerebral artery occlusion of 60 min followed by reperfusion for 1 h, 6 h, and 24 h was employed in the present study to produce ischemia and reperfusion-induced cerebral injury in rats. Tests were employed to assess the functional outcome of the sensorimotor center activity in the brain, through a set of modified neurological severity scores used to assess motor and exploratory capacity 24 h, 14, and 28 days after receiving cellular therapy via tail vein. In our animal model of stroke, transplanted cells migrated to the ischemic focus, infarct volume decreased, and motor deficits improved. Therefore, we concluded that these cells appear to have beneficial effects on the ischemic brain, possibly based on their ability to enhance endogenous repair mechanisms.

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“…Yet, these developing technologies face obstacles, such as bioethical concerns and misrepresentations, as well as exploitations by the unscrupulous media and business sector, preventing them from reaching full beneficial capacity. Accordingly, a common theme resonating throughout these papers is a push to increase translational research of cell-based therapeutics for clinical applications that will allow scientifically sound assessments of cell therapy, delineating hype from hope [40][41][42][43][44][45][46][47][48]. Forthcoming updates on the ongoing clinical trials of cell therapy will provide valuable information on which to build the future of stem cell research and therapeutic applications.…”

Section: Resultsmentioning

confidence: 99%

An update on stem cell therapy for neurological disorders: cell death pathways as therapeutic targets

et al. 2017

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Here, we compiled ten papers detailing recent strides in the field of stem cell therapy, which address critical issues relevant to pursuing this experimental treatment for clinical applications in brain disorders. Topics include efficacy, safety, and mechanism of action underlying cell therapy, emerging technologies and combination pharmacotherapies with cell transplantation directed at improving the functional outcomes, and evaluation of key translational components of advancing novel therapeutics to the clinic, including the need for vis-à-vis comparisons with the gold standard of treatment post-injury (i.e., physical rehabilitation). A bioethics paper is also incorporated here to further appreciate the current status of cell therapy in the community setting. The overall goal of this special volume is to provoke a meaningful assessment of the lessons we have learned in recent years and to use such knowledge to carefully translate safe and effective applications of cell therapy, and its mechanism of action for the treatment of neurological disorders.

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Stroke Therapy: the Potential of Amniotic Fluid-Derived Stem Cells

Cited by 11 publications

References 42 publications

Neuroprotective effects of human amniotic fluid stem cells-derived secretome in an ischemia/reperfusion model

Neuroprotective effects of human amniotic fluid stem cells-derived secretome in an ischemia/reperfusion model

Intravenous Grafts of Human Amniotic Fluid-Derived Stem Cells Reduce Behavioral Deficits in Experimental Ischemic Stroke

An update on stem cell therapy for neurological disorders: cell death pathways as therapeutic targets

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