Stroke

Rao, Ting

doi:10.5005/jp/books/11411_6

Cited by 1 publication

(2 citation statements)

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“…This adaptability has paved the way for exploring SERSbased wearable sensors for real-time, noninvasive monitoring of diverse biochemical markers and components present in human sweat. 16 Multiplexed analysis using SERS droplet assays offers advantages in the field of analytical chemistry, as it enables the simultaneous detection of multiple analytes within a single assay, dramatically increasing efficiency and throughput. Vedelago and his team recently developed a multiplex SERS micro assay capable of detecting specific variants of SARS-CoV-2.…”

Section: ■ Introductionmentioning

confidence: 99%

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A Wettability Contrast SERS Droplet Assay for Multiplexed Analyte Detection

Puravankara,

Manjeri,

Kulkarni

et al. 2024

Anal. Chem.

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Droplet assay platforms have emerged as a significant methodology, providing distinct advantages such as sample compartmentalization, high throughput, and minimal analyte consumption. However, inherent complexities, especially in multiplexed detection, remain a challenge. We demonstrate a novel strategy to fabricate a plasmonic droplet assay platform (PDAP) for multiplexed analyte detection, enabling surfaceenhanced Raman spectroscopy (SERS). PDAP efficiently splits a microliter droplet into submicroliter to nanoliter droplets under gravity-driven flow by wettability contrast between two distinct regions. The desired hydrophobicity and adhesive contrast between the silicone oil-grafted nonadhesive hydrophilic zone with gold nanoparticles is attained through (3-aminopropyl) triethoxysilane (APTES) functionalization of gold nanoparticles (AuNPs) using a scotch-tape mask. The wettability contrast surface facilitates the splitting of aqueous droplets with various surface tensions (ranging from 39.08 to 72 mN/m) into ultralow volumes of nanoliters. The developed PDAP was used for the multiplexed detection of Rhodamine 6G (Rh6G) and Crystal Violet (CV) dyes. The limit of detection for 120 nL droplet using PDAP was found to be 134 pM and 10.1 nM for Rh6G and CV, respectively. These results align with those from previously reported platforms, highlighting the comparable sensitivity of the developed PDAP. We have also demonstrated the competence of PDAP by testing adulterant spiked milk and obtained very good sensitivity. Thus, PDAP has the potential to be used for the multiplexed screening of food adulterants.

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Section: ■ Introductionmentioning

confidence: 99%

“…AuNPs were synthesized using the previously reported citrate reduction method. 16 50 mL of 1 mM HAuCl 4 • 3H 2 O was heated at 90 °C. To this, 40 mM of trisodium citrate solution was added quickly.…”

Section: ■ Introductionmentioning

confidence: 99%