STRoe deer: A validated forensic STR profiling system for the European roe deer (Capreolus capreolus)

Morf, Nadja; Kopps, Anna M.; Nater, Alexander; Lendvay, Bertalan; Vasiljevic, Nina; Webster, Lucy M.I.; Fautley, Richard Guy; Ogden, Rob; Krätzer, A.

doi:10.1016/j.fsiae.2021.100023

Cited by 11 publications

(14 citation statements)

References 36 publications

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“…antlers, meat, trace blood samples) associated with the suspect who might have committed the offence (Poetsch et al 2001). Although, for forensic purposes, a validated DNA profiling system "STRoe deer" for European roe deer consisting of 12 unlinked STR and two sexing markers has been recently developed (Morf et al 2021), the SNP panel described in our study can be used as the complementary tool, especially in labs which have no facility for STR genotyping. Moreover, the overall cost of genotyping an animal by STR is much higher than by SNP (https://lgm.izoo.…”

Section: Discussionmentioning

confidence: 99%

SNP panel for evaluation of genetic variability and relationship in roe deer ( Capreolus capreolus)

Oleński

Zalewski

Kamiński

2023

Polish Journal of Veterinary Sciences

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Blood samples from forty-six roe deer (Capreolus capreolus) acquired during officially approved hunting in six hunting divisions throughout Poland were used to isolate the genomic DNA. All individuals were genotyped by MD_Bovine BeadChip (Illumina) for 46.750 Single Nucleotide Polymorphism (SNP) markers. SNPs of inappropriate clusters, with a marker call rate lower than 90% and with a minor allele frequency (MAF) lower than 0.01, located on sex chromosomes and mitochondrial DNA, were removed. Altogether, 21.033 SNP markers were included for further analysis. Observed and expected heterozygosity amounted to 0.098 and 0.119, respectively. Among 21.033 markers, a panel of 148 SNPs were selected for relationship analysis. They were unlinked and had a MAF higher than 0.2. This set of SNPs showed a probability of parentage exclusion of 1.29x10 -6 and 2.37x10 -19 for one, and two known parents, respectively. The probability of identity was estimated at 1.8x10 -40 . The probabilities obtained in this study are sufficient for the monitoring and effective management of the genetic diversity of roe deer in Poland and is a cost-effective complementary tool for forensic applications.

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Section: Discussionmentioning

confidence: 99%

SNP panel for evaluation of genetic variability and relationship in roe deer ( Capreolus capreolus)

Oleński

Zalewski

Kamiński

2023

Polish Journal of Veterinary Sciences

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“…DNA from tissue and blood samples was extracted using the DNeasy ® Blood and Tissue kit (Qiagen). DNA from blood swab samples was extracted using a chelex protocol followed by a Qiagen PCR purification kit (Qiagen, Switzerland) as described in Morf et al (2021).…”

Section: Methodsmentioning

confidence: 99%

“…A representative subset (n = 152) of the available samples were randomly selected for mtDNA sequencing in order to ensure that each A total of 316 samples were genotyped using a species-specific microsatellite panel consisting of 13 tetrameric microsatellites and two sex-specific markers shown to be polymorphic, following the method described in (Morf et al, 2021). The PCR products were resolved using an ABI 3100 Genetic Analyzer (Thermo Fisher Scientific) and allele sizes called using GeneMapper ID-X version 1.4.…”

Section: Genotyping and Mitochondrial Dna Sequencingmentioning

confidence: 99%

Phylogeography and population genetic structure of the European roe deer in Switzerland following recent recolonization

Vasiljevic

Morf

Senn

et al. 2022

Ecology and Evolution

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In the early 1800s, the European roe deer (Capreolus capreolus) was probably extirpated from Switzerland, due to overhunting and deforestation. After a federal law was enacted in 1875 to protect lactating females and young, and limiting the hunting season, the roe deer successfully recovered and recolonized Switzerland. In this study, we use mitochondrial DNA and nuclear DNA markers to investigate the recolonization and assess contemporary genetic structure in relation to broad topographic features, in order to understand underlying ecological processes, inform future roe deer management strategies, and explore the opportunity for development of forensic traceability tools. The results concerning the recolonization origin support natural, multidirectional immigration from neighboring countries. We further demonstrate that there is evidence of weak genetic differentiation within Switzerland among topographic regions. Finally, we conclude that the genetic data support the recognition of a single roe deer management unit within Switzerland, within which there is a potential for broad‐scale geographic origin assignment using nuclear markers to support law enforcement.

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“…The various deer species are also usually much-prized in Europe, although their economic importance can vary from country to country. Red deer (Cervus elaphus), roe deer (Capreolus capreolus) and fallow deer (Dama dama) are not only the most common game species in Europe, hunted for meat and trophies [14], but can also provide social values, even as mythological ("Wonder Deer") or emblematic members ("Royal Game") of the natural megafauna [15,16]. In several countries, such as Hungary, there are limits on their harvest as game animals.…”

Section: Introductionmentioning

confidence: 99%

“…In spite of the efforts in related research, this field is quite heterogeneous, and the range of forensically validated assays are relatively limited [6,[34][35][36]. Although, recently developed marker sets for forensic applications usually incorporate genetic markers for sex determination [14,15,17], there are only few published validated assays simultaneously detecting sex and species on cervids [31].…”

Section: Introductionmentioning

confidence: 99%

Molecular Sexing and Species Detection of Antlered European Hunting Game for Forensic Purposes

Zenke

Zorkóczy

Lehotzky³

et al. 2022

Animals

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Molecular sexing techniques are widely applied in conservation biology, although the range of forensically validated methods is fairly limited. The primary aim of this work was to develop forensically validated assays, using two PCR panels for sex and species assignment for the abundant antlered European game species: red deer (Cervus elaphus), roe deer (Capreolus capreolus) and fallow deer (Dama dama). Segments of the SRY and Amelogenin X/Y genes for sex determination, additionally species-specific cytochrome b regions for species detection were targeted and separately amplified in two multiplex reactions. These assays can reliably analyze trace amounts of DNA. The results of both can easily be visualized and interpreted practically, either on agarose gel or by capillary electrophoresis. These simple, fast molecular assays are able to affect the early-stage resolution of disputed or unsolved poaching cases, without the need of individualization or sequencing of forensic samples.

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STRoe deer: A validated forensic STR profiling system for the European roe deer (Capreolus capreolus)

Cited by 11 publications

References 36 publications

SNP panel for evaluation of genetic variability and relationship in roe deer ( Capreolus capreolus)

SNP panel for evaluation of genetic variability and relationship in roe deer ( Capreolus capreolus)

Phylogeography and population genetic structure of the European roe deer in Switzerland following recent recolonization

Molecular Sexing and Species Detection of Antlered European Hunting Game for Forensic Purposes

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