STRO-1 positive cell expansion during osteogenic differentiation: A comparative study of three mesenchymal stem cell types of dental origin

Perczel-Kovách, Katalin; Hegedűs, Orsolya; Földes, Anna; Sang-Ngoen, Thanyaporn; Kálló, Karola; Steward, Martin C.; Varga, Gábor; Nagy, Krisztina

doi:10.1016/j.archoralbio.2020.104995

Cited by 16 publications

(23 citation statements)

References 75 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Its potential differentiation ability plays an important role in the process of inflammation, repair, cell necrosis, and regeneration of dental pulp tissue [32]. Consistently with previous reports [33,34], DPSCs exhibited multi-directional differentiation ability (adipogenesis and osteogenesis) and have strong expression of stem cell surface markers (CD73, CD90, and CD105). Moreover, cultured cells were also measured by other hematopoietic markers (CD14, CD20, CD34, and CD45) to rule out the possibility that it contains hematopoietic stem cells.…”

Section: Discussionsupporting

confidence: 84%

H2O2 gel bleaching induces cytotoxicity and pain conduction in dental pulp stem cells via intracellular reactive oxygen species on enamel/dentin disc

et al. 2021

View full text Add to dashboard Cite

Background Bleaching is widely accepted for improving the appearance of discolored teeth; however, patient compliance is affected by bleaching-related complications, especially bleaching sensitivity. This study aimed to investigate the role of reactive oxygen species (ROS) in cytotoxicity and pain conduction activated by experimental tooth bleaching. Methods Dental pulp stem cells with or without N-acetyl-L-cysteine (NAC), an ROS scavenger, were cultured on the dentin side of the enamel/dentin disc. Subsequently, 15% (90 min) and 40% (30 min) bleaching gels were painted on the enamel surface. Cell viability, intracellular ROS, Ca2+, adenosine triphosphate (ATP), and extracellular ATP levels were evaluated using the Cell Counting Kit-8 assay, 2’,7’-dichlorodihydrofluorescein diacetate, CellROX, fura-3AM fluorescence assay, and ATP measurement kit. The rat incisor model was used to evaluate in vivo effects after 0, 1, 3, 7, and 30 days of bleaching. Changes in gene and protein expression of interleukin 6 (IL-6), tumor necrosis factor-alpha (TNFα), transient receptor potential ankyrin 1 (TRPA1), and Pannexin1 (PANX1) in dental pulp stem cells and pulp tissue were detected through RT-PCR, western blotting, and immunofluorescence. Results The bleaching gel suppressed dental pulp stem cell viability and extracellular ATP levels and increased intracellular ROS, Ca2+, and intracellular ATP levels. The mRNA and protein expression of IL-6, TNFα, TRPA1, and PANX1 were up-regulated in vitro and in vivo. Furthermore, the 40% gel had a stronger effect than the 15% gel, and NAC ameliorated the gel effects. Conclusions Our findings suggest that bleaching gels induce cytotoxicity and pain conduction in dental pulp stem cells via intracellular ROS, which may provide a potential therapeutic target for alleviating tooth bleaching nociception.

show abstract

Section: Discussionsupporting

confidence: 84%

H2O2 gel bleaching induces cytotoxicity and pain conduction in dental pulp stem cells via intracellular reactive oxygen species on enamel/dentin disc

et al. 2021

View full text Add to dashboard Cite

show abstract

“…During osteogenic differentiation, Nestin mRNA expression was increased corresponding with the increase of BMP2 and OCN in human dental follicle cells, human stem cell isolated from apical papilla and murine odontoblast‐like cells (Bakopoulou et al, 2013; Man et al, 2012; Morsczeck et al, 2005). On the contrary, a study on periodontal ligament stem cells and dental pulp stem cells revealed that the similar Nestin expression levels were observed after osteogenic differentiation (Perczel‐Kovach et al, 2021). The present study demonstrated the reduction of NESTIN mRNA expression in Jagged1 treatment and TGF‐beta pre‐treatment in osteogenic induction medium.…”

Section: Discussionmentioning

confidence: 98%

Dorsomorphin attenuates Jagged1‐induced mineralization in human dental pulp cells

et al. 2021

View full text Add to dashboard Cite

Aim To investigate whether TGF‐β/BMP signalling participates in Jagged1‐induced osteogenic differentiation in human dental pulp cells (hDPs). Methodology Bioinformatic analysis of publicly available RNA sequencing data of Jagged1‐treated hDPs was performed using NetworkAnalyst. The mRNA expression was validated using real‐time polymerase chain reaction. hDPs were seeded on Jagged1 immobilized surfaces in the presence or absence of TGF‐β or BMP inhibitor. Osteogenic differentiation was evaluated using alkaline phosphatase staining, osteogenic marker gene expression and mineralization assay. Statistical analyses were performed using a Kruskal–Wallis test, followed by a pairwise comparison for more than three group comparison. Mann–Whitney U‐test was employed for two group comparison. The statistical significance was considered at p < .05. Results Jagged1 treatment in growth medium significantly promoted TGFB1, TGFB2 and TGFB3 whilst significantly inhibited BMP2, BMP4 and BMP6 mRNA expression (p < .05). In osteogenic induction medium, Jagged1 significantly up‐regulated TGFB1, TGFB2 and TGFB3 at days 1 and 3 (p < .05). Pre‐treatment with TGF‐β1, TGF‐β2 or TGF‐β3 prior to osteogenic induction resulted in the significant increase of osteogenic marker gene expression, collagen type 1 protein expression, alkaline phosphatase enzymatic activity and mineral deposition (p < .05). However, TGF‐β signalling inhibition with SB431542 (4 μmol L−1) or SB505124 (47 and 129 nmol L−1) failed to attenuate the effect of Jagged1‐induced osteogenic differentiation in hDPs. Dorsomorphin (4 and 8 μmol L−1) treatment significantly abolished the effect of Jagged1 on mineralization by hDPs (p < .05). Conclusion Notch signalling activation by Jagged1 modulated TGF‐β and BMP ligand expression. Dorsomorphin, but not TGF‐β receptor inhibitor, attenuated Jagged1‐induced osteogenic differentiation in hDPs.

show abstract

“…DPSCs are known to share common properties with bone marrow mesenchymal stromal cells [8], expressing CD73, CD90, CD105 and Stro-1 cell surface markers [9], mesenchymal stem cell markers such as nestin and vimentin [10][11][12], and also osteogenic markers such as osteonectin and bone sialoprotein [8,13]. Furthermore, they also show similar, but not identical, differentiation capabilities with other mesenchymal stem cells [8,13].…”

Section: Introductionmentioning

confidence: 99%

“…Cells in these cultures uniformly show stem cell/progenitor cell marker proteins including nestin and vimentin, but they are also somewhat heterologous, as indicated by the expression of Stro-1, which is generally regarded as a mesenchymal stem cell marker. Stro-1 is exhibited by 10-20% of the cell population, and its expression is dependent on the type of applied medium, FBS concentration and passage number [12]. These cells are clearly regarded as multipotent, but the differentiation potential does not apply only to the small portion of Stro-1 positive cells in the DPSC cultures.…”

Section: Introductionmentioning

confidence: 99%

“…These cells are clearly regarded as multipotent, but the differentiation potential does not apply only to the small portion of Stro-1 positive cells in the DPSC cultures. Previous quantitative studies by us and by others have clearly demonstrated that the majority of cells in the culture are able to differentiate into both osteogenic [10][11][12]17] and neurogenic [10,11,20,21] lineages. Obviously, many of these are not Stro-1 positive, but are nonetheless able to show multipotency for differentiation.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Culturing and Scaling up Stem Cells of Dental Pulp Origin Using Microcarriers

et al. 2021

Self Cite

View full text Add to dashboard Cite

Ectomesenchymal stem cells derived from the dental pulp are of neural crest origin, and as such are promising sources for cell therapy and tissue engineering. For safe upscaling of these cells, microcarrier-based culturing under dynamic conditions is a promising technology. We tested the suitability of two microcarriers, non-porous Cytodex 1 and porous Cytopore 2, for culturing well characterized dental pulp stem cells (DPSCs) using a shake flask system. Human DPSCs were cultured on these microcarriers in 96-well plates, and further expanded in shake flasks for upscaling experiments. Cell viability was measured using the alamarBlue assay, while cell morphology was observed by conventional and two-photon microscopies. Glucose consumption of cells was detected by the glucose oxidase/Clark-electrode method. DPSCs adhered to and grew well on both microcarrier surfaces and were also found in the pores of the Cytopore 2. Cells grown in tissue culture plates (static, non-shaking conditions) yielded 7 × 105 cells/well. In shake flasks, static preincubation promoted cell adhesion to the microcarriers. Under dynamic culture conditions (shaking) 3 × 107 cells were obtained in shake flasks. The DPSCs exhausted their glucose supply from the medium by day seven even with partial batch-feeding. In conclusion, both non-porous and porous microcarriers are suitable for upscaling ectomesenchymal DPSCs under dynamic culture conditions.

show abstract

STRO-1 positive cell expansion during osteogenic differentiation: A comparative study of three mesenchymal stem cell types of dental origin

Cited by 16 publications

References 75 publications

H2O2 gel bleaching induces cytotoxicity and pain conduction in dental pulp stem cells via intracellular reactive oxygen species on enamel/dentin disc

H2O2 gel bleaching induces cytotoxicity and pain conduction in dental pulp stem cells via intracellular reactive oxygen species on enamel/dentin disc

Dorsomorphin attenuates Jagged1‐induced mineralization in human dental pulp cells

Culturing and Scaling up Stem Cells of Dental Pulp Origin Using Microcarriers

Contact Info

Product

Resources

About