STRIP2, a member of the striatin‐interacting phosphatase and kinase complex, is implicated in lung adenocarcinoma cell growth and migration

Qiu, Limin; Sun, Yunhao; Chen, Tingting; Chen, Jinjin; Ma, Hu

doi:10.1002/2211-5463.12785

Cited by 9 publications

(10 citation statements)

References 33 publications

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“…Among the 7 genes that were associated with both sphere induction and poor prognosis of HCC, the expression of PBLD , STRIP2 , and LOC344887 has also been correlated with poor prognosis of HCC, lung adenocarcinoma, and non-small cell lung cance [ 40 , 41 ]. Moreover, these genes are associated with EMT and invasive potential of cells [ 40 , 42 , 43 ]. Recently, ATP6V0D2 , a proton transporter, which showed the highest M value in our study (Fig.…”

Section: Discussionmentioning

confidence: 99%

Elevated expression of RAB3B plays important roles in chemoresistance and metastatic potential of hepatoma cells

et al. 2022

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Background Cancer stem cells (CSCs) are thought to play important roles in carcinogenesis, recurrence, metastasis, and therapy-resistance. We have successfully induced cancer stem-like sphere cells (CSLCs) which possess enhanced chemoresistance and metastatic potential. To enable the development of targeted therapy against CSLCs, we identified a gene responsible for this phenotype in CSLC. Methods Human hepatoma cell line SK-HEP-1 was used for CSLC induction with a unique sphere inducing medium, and HuH-7 cells were used as non-sphere forming cells in the same condition. RNA-sequencing was performed followed by validation with quantitative RT-PCR and western blotting. Knockdown experiments were done by using CRISPR-Cas9 genome-editing, and the rescue experiments were performed using the expressing plasmid vector. Chemoresistance and liver metastasis of the cells, was studied following the splenic injection of cells to severely immune deficient mice and evaluated using the MTS assay. Quantification of exosomes in the medium was done using ELISA. Results RAB3B was identified as an up-regulated gene in both CSLCs and prognostically poor hepatocellular carcinoma (HCC) by RNA-sequencing. RAB3B-KD cells showed altered CSLC phenotypes such as sphere formation, chemoresistance, and metastatic potentials, and those were rescued by RAB3B complementation. Increased exosome secretion was observed in CSLCs, and it was not observed in the RAB3B-KD cells. In addition, the RAB3B expression correlated with the expression of ABCG2, APOE, LEPR, LXN, and TSPAN13. Conclusion The up regulation of RAB3B may play an important role in the chemoresistance and metastatic potential of CSLCs.

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Section: Discussionmentioning

confidence: 99%

Elevated expression of RAB3B plays important roles in chemoresistance and metastatic potential of hepatoma cells

et al. 2022

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“…Strip1 is one of the scaffolding proteins in the STRIPAK complex, which is involved in embryogenesis and development ( Lant et al, 2015 ; Sakuma et al, 2015 , 2016 ; Bazzi et al, 2017 ; Pal et al, 2017 ; Zheng et al, 2017 ). Strip2, another scaffolding protein in the STRIPAK complex, is also reported to play roles in lung adenocarcinoma, smooth muscle, embryonic stem cells, and inner ear HCs ( Sabour et al, 2017 ; Dai et al, 2019 ; Pisciottano et al, 2019 ; Qiu et al, 2020 ). In the cochlea, Strip2 is reported to be expressed in cochlear HCs, and mice lacking Strip2 have decreased neural response amplitudes and reduced numbers of afferent synapses ( Pisciottano et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

“…Strip2 (also called FAM40B), another Strip proteins, is also one of the scaffold proteins in the STRIPAK complex ( Hwang and Pallas, 2014 ). Strip2 is also reported to play important roles in cell survival, growth, proliferation, differentiation and migration ( Sabour et al, 2017 ; Dai et al, 2019 ; Pisciottano et al, 2019 ; Qiu et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Characterization of Strip1 Expression in Mouse Cochlear Hair Cells

et al. 2021

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Striatin-interacting protein 1 (Strip1) is a core component of the striatin interacting phosphatase and kinase (STRIPAK) complex, which is involved in embryogenesis and development, circadian rhythms, type 2 diabetes, and cancer progression. However, the expression and role of Strip1 in the mammalian cochlea remains unclear. Here we studied the expression and function of Strip1 in the mouse cochlea by using Strip1 knockout mice. We first found that the mRNA and protein expression of Strip1 increases as mice age starting from postnatal day (P) 3 and reaches its highest expression level at P30 and that the expression of Strip1 can be detected by immunofluorescent staining starting from P14 only in cochlear HCs, and not in supporting cells (SCs). Next, we crossed Strip1 heterozygous knockout (Strip +/−) mice to obtain Strip1 homozygous knockout (Strip1−/−) mice for studying the role of Strip1 in cochlear HCs. However, no Strip1−/− mice were obtained and the ratio of Strip +/− to Strip1+/+ mice per litter was about 2:1, which suggested that homozygous Strip1 knockout is embryonic lethal. We measured hearing function and counted the HC number in P30 and P60 Strip +/− mice and found that they had normal hearing ability and HC numbers compared to Strip1+/+ mice. Our study suggested that Strip1 probably play important roles in HC development and maturation, which needs further study in the future.

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“…Lung cancer is one of the most common disease of cancer-related death in the past ten years (2008-2017) [1], and the ve-year survival rate for patients with lung cancer is only 19%. Lung adenocarcinoma (LUAC) accounts for 40% of lung cancer types, with more than 1 million deaths annually worldwide [2,3]. In recent decades, circRNAs have great potential as prognostic and diagnostic biomarkers for LUAC [4].…”

Section: Introductionmentioning

confidence: 99%

Advances in Research on the circRNA-miRNA-mRNA regulatory network in lung adenocarcinoma by analysis of microarray data

Yao

Pang

et al. 2020

Preprint

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Background: The present researches indicate that circular RNAs (circRNAs) play pivotal roles in the generation and development of human cancers. However, function of circRNAs in lung adenocarcinoma is still unknown. Herein, we focused our study on the regulation mechanism of circRNAs in lung adenocarcinoma (LUAC)Methods: CircRNAs-related data can be downloaded from Gene Expression Omnibus(GEO) microarray databases. Relevant expression data of miRNAs and mRNAs were obtained from The Cancer Genome Atlas (TCGA) databases. The differentially expressed circRNAs (DEcircRNAs) were obtained by the robust rank aggregation method, and constructed a ceRNA network with circRNA-miRNA pairs and miRNA-mRNA pairs. The function and pathway enrichment of different genes were analyzed, and the protein-protein interaction (PPI) was predicted by String software, and the subnetwork management module was constructed by MCODE plugin. The differentially expressed mRNAs (DEmRNAs) were used to establish survival analysis by R software.Results: A total of 19 up-regulated circRNAs, 45 down-regulated circRNAs, 98 up-regulated miRNAs, 18 down-regulated miRNAs, 2002 up-regulated mRNAs, and 553 down-regulated mRNAs were obtained in lung adenocarcinoma. The circRNA-miRNA-mRNA network was constructed by six circRNAs (hsa_circ_0003528, hsa_circ_0004315, hsa_circ_0005699, hsa_circ_0002588, hsa_circ_0005777, hsa_circ_0027033), 19 miRNAs and 33 mRNAs. GO and KEGG pathway analysis indicated that DEmRNAs might be related to the progression of LUAC. The PPI network was constructed and four hub genes (CEP55, CHEK1, CDC25A, KIF23) were obtained from the network, and four hub genes were found to be associated with the same miRNA and circRNA, including hsa_circ_0004315/hsa-miR-195/CEP55, CHEK1, CDC25A, KIF23. Three hub genes (CEP55, CHEK1, KIF23) were significantly associated with LUAC survival and prognosis, and the overall survival rate of lung adenocarcinoma was decreased in high-expression genes compared with low-expression genes.Conclusion: Our finding indicated that circRNA-miRNA- mRNA axis paly key role in the pathogenesis of lung adenocarcinoma, and some circRNAs could be considered as a biomarker and target for the diagnosis and therapy of LUAC patients.

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STRIP2, a member of the striatin‐interacting phosphatase and kinase complex, is implicated in lung adenocarcinoma cell growth and migration

Cited by 9 publications

References 33 publications

Elevated expression of RAB3B plays important roles in chemoresistance and metastatic potential of hepatoma cells

Elevated expression of RAB3B plays important roles in chemoresistance and metastatic potential of hepatoma cells

Characterization of Strip1 Expression in Mouse Cochlear Hair Cells

Advances in Research on the circRNA-miRNA-mRNA regulatory network in lung adenocarcinoma by analysis of microarray data

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