STRIP1, a core component of STRIPAK complexes, is essential for normal mesoderm migration in the mouse embryo

Bazzi, Hisham; Soroka, Ekaterina; Alcorn, Heather L.; Anderson, Kathryn V.

doi:10.1073/pnas.1713535114

Cited by 29 publications

(34 citation statements)

References 59 publications

(66 reference statements)

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“…Over the past few years, extensive functional and mechanistic research has been conducted to resolve the framework of the Striatin Interacting Phosphatase and Kinase (STRIPAK) complex. The accumulated findings have linked specific components of the complex to various biological functions including vesicular trafficking (Zhang et al, 2013;Lant et al, 2015), Golgi assembly (Kean et al, 2011), Hippo signaling (Ribeiro et al, 2010;Zheng et al, 2017), autophagy (Huang et al, 2017), cell migration (Madsen et al, 2015;Bazzi et al, 2017), and cell cycle control (Cornils et al, 2011;Frost et al, 2012;Kazmierczak-Baranska et al, 2015;Pandey et al, 2017). Substantiated by these findings, the STRIPAK complex is supervising embryogenesis and development (Lant et al, 2015;Madsen et al, 2015;Sakuma et al, 2015Sakuma et al, , 2016Bazzi et al, 2017;Pal et al, 2017;Zheng et al, 2017), circadian rhythms (Andreazza et al, 2015), type 2 diabetes (Chursa et al, 2017), and progression of cancer (Wong et al, 2014;Zhang et al, 2014;Madsen et al, 2015;Huang et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…The Strip1 homolog in the fruit fly has also been linked to cell proliferation by antagonizing Hippo signaling and by supporting RAS/MAPK signaling (Ashton-Beaucage et al, 2014). In the mouse embryo, loss of Strip1 arrests mesoderm migration after the gastrulation epithelial-to-mesenchymal transition (Bazzi et al, 2017). Indeed, STRIP1 has been shown to regulate cytoskeleton dynamics and cell migration on several occasions (Bai et al, 2011;Sakuma et al, 2015Sakuma et al, , 2016Suryavanshi et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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The STRIPAK Complex Regulates Response to Chemotherapy Through p21 and p27

Rodriguez-Cupello

Dam

Serini

et al. 2020

Front. Cell Dev. Biol.

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The STRIPAK complex has been linked to a variety of biological processes taking place during embryogenesis and development, but its role in cancer has only just started to be defined. Here, we expand on previous work indicating a role for the scaffolding protein STRIP1 in cancer cell migration and metastasis. We show that cell cycle arrest and decreased proliferation are seen upon loss of STRIP1 in MDA-MB-231 cells due to the induction of cyclin dependent kinase inhibitors, including p21 and p27. We demonstrate that p21 and p27 induction is observed in a subpopulation of cells having low DNA damage response and that the p21 high /γH2AX low ratio within single cells can be rescued by depleting MST3&4 kinases. While the loss of STRIP1 decreases cell proliferation and tumor growth, cells treated with low dosage of chemotherapeutics in vitro paradoxically escape therapy-induced senescence and begin to proliferate after recovery. This corroborates with already known research on the dual role of p21 and indicates that STRIP1 also plays a contradictory role in breast cancer, suppressing tumor growth, but once treated with chemotherapeutics, allowing for possible recurrence and decreased patient survival.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The STRIPAK Complex Regulates Response to Chemotherapy Through p21 and p27

Rodriguez-Cupello

Dam

Serini

et al. 2020

Front. Cell Dev. Biol.

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“…The cells of the node and notochordal plate are also characterized by constricted apical surfaces, which face the outside and thus can be stained with fluorescence-conjugated Phalloidin to mark F-Actin at the apical constrictions. Using these reagents as examples, the combination of T and F-Actin staining by WMIF provides a representation of the node and notochordal plate in 3D in gastrulating mouse embryos as we demonstrate in Step 2 8 . However, markers of cilia, such as ARL13B or acetylated tubulin, as well as other markers of the node and notochordal plate, such as FOXA2, can also be used to perform WMIF on developing mouse embryos 3,4 .…”

mentioning

confidence: 69%

“…They were identifiable with their shorter cilia. Therefore, SEM was important to reveal the node morphogenesis defects in Strip1 mutants 8 . We have also used SEM in previous studies to show the absence of cilia in the embryonic node of mutants that lacked centrioles, which provide the template for cilia 9 .…”

Section: Representative Resultsmentioning

confidence: 99%

“…Because of the attributes of location (surface), shape (cup) and possessing distinct outer cellular structures (cilia), SEM has traditionally been used to visualize the node and notochordal plate and study their formation and structure 2,7 . SEM is also used to study the changes in the structure of the node itself or the cilia on its cells in mutations that affect gastrulation, morphogenesis, as well as cilia formation 8,9,10 . SEM is a technique that utilizes a focused beam of electrons to interrogate the topological ultrastructure of the outer surface of materials such as biological specimens…”

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confidence: 99%

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Visualizing the Node and Notochordal Plate In Gastrulating Mouse Embryos Using Scanning Electron Microscopy and Whole Mount Immunofluorescence

Xiao

Nitsche

Bazzi

2018

JoVE

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The post-implantation mouse embryo undergoes major shape changes after the initiation of gastrulation and morphogenesis. A hallmark of morphogenesis is the formation of the transient organizers, the node and notochordal plate, from cells that have passed through the primitive streak. The proper formation of these signaling centers is essential for the development of the body plan and techniques to visualize them are of high interest to mouse developmental biologists. The node and notochordal plate lie on the ventral surface of gastrulating mouse embryos around embryonic day (E) 7.5 of development. The node is a cup-shaped structure whose cells possess a single slender cilium each. The proper subcellular localization and rotation of the cilia in the node pit determines left-right asymmetry. The notochordal plate cells also possess single cilia albeit shorter than those of the node cells. The notochordal plate forms the notochord which acts as an important signaling organizer for somitogenesis and neural patterning. Because the cells of the node and notochordal plate are transiently present on the surface and possess cilia, they can be visualized using scanning electron microscopy (SEM). Among other techniques used to visualize these structures at the cellular level is whole mount immunofluorescence (WMIF) using the antibodies against the proteins that are highly expressed in the node and notochordal plate. In this report, we describe our optimized protocols to perform SEM and WMIF of the node and notochordal plate in developing mouse embryos to help in the assessment of tissue shape and cellular organization in wild-type and gastrulation mutant embryos.

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Subcellular dynamics of variants of SG2NA in NIH3T3 fibroblasts

Chauhan

Gupta

Jain

et al. 2019

Cell Biology International

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SG2NA, a WD40 repeat protein of the Striatin subfamily, has four splicing and one messenger RNA edit variants. It is fast emerging as a scaffold for multimeric signaling complexes with roles in tissue development and disease. The green fluorescent protein (GFP)‐tagged variants of SG2NA were ectopically expressed in NIH3T3 cells and their modulation by serum and GSK3β‐ERK signaling were monitored. The 87, 78, and 35 kDa variants showed a biphasic modulation by serum till 24 h but the 52 kDa variant remained largely unresponsive. Inhibition of phosphatases by okadaic acid increased the levels of the endogenous 78 kDa and the ectopically expressed GFP‐tagged 87 and 78 kDa SG2NAs. Contrastingly, okadaic acid treatment reduced the level of GFP‐tagged 35 kDa SG2NA, suggesting differential modes of their stability through phosphorylation‐dephosphorylation. The inhibition of GSK3β by LiCl showed a gradual decrease in the levels of 78 kDa. In the case of the other variants viz, GFP‐tagged 35, 52, and 87 kDa, inhibition of GSK3β caused an initial increase followed by a decrease with a subtle difference in kinetics and intensities. Similar results were also seen upon inhibition of GSK3β by small interfering RNA. All the variants showed an increase followed by a decrease upon inhibition of extracellular‐signal‐regulated‐kinase (ERK). These variants are localized in the plasma membrane, endoplasmic reticulum, mitochondria, and the nucleus with different propensities and no discernable subcellular distribution was seen upon stimulation by serum and the inhibition of phosphatases, GSK3β, and ERK. Taken together, the variants of SG2NA are modulated by the kinase‐phosphatase network in a similar but characteristic manner.

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STRIP1, a core component of STRIPAK complexes, is essential for normal mesoderm migration in the mouse embryo

Cited by 29 publications

References 59 publications

The STRIPAK Complex Regulates Response to Chemotherapy Through p21 and p27

The STRIPAK Complex Regulates Response to Chemotherapy Through p21 and p27

Visualizing the Node and Notochordal Plate In Gastrulating Mouse Embryos Using Scanning Electron Microscopy and Whole Mount Immunofluorescence

Subcellular dynamics of variants of SG2NA in NIH3T3 fibroblasts

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