String test of hypervirulent Klebsiella pneumonia

Kumabe, Ayako; Kenzaka, Tsuneaki

doi:10.1093/qjmed/hcu124

Cited by 19 publications

(16 citation statements)

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“…Colonies grown on 5% sheep blood agar were touched with an inoculation loop and then the loop was lifted from the agar surface. Each strain was defined as mucoid or hypermucoviscous (HV) when a string‐like growth or a string longer than 5 mm was observed respectively (Kumabe and Kenzaka ).…”

Section: Methodsmentioning

confidence: 99%

Biofilm formation and antibiotic resistance inKlebsiella pneumoniaeurinary strains

et al. 2017

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Section: Methodsmentioning

confidence: 99%

Biofilm formation and antibiotic resistance inKlebsiella pneumoniaeurinary strains

et al. 2017

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“…In order to test for a putative hypervirulence or hypermucoviscous phenotype, we established the so-called ‘string test’ [ 21 ]. The hypervirulent K. pneumoniae isolate of ST2398 possessing a K2 capsule was used as a positive control [ 22 ].…”

Section: Methodsmentioning

confidence: 99%

Genome-based analysis of Carbapenemase-producing Klebsiella pneumoniae isolates from German hospital patients, 2008-2014

Becker

Kaase

Pfeifer

et al. 2018

Antimicrob Resist Infect Control

106

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BackgroundBy using whole genome sequence data we aimed at describing a population snapshot of carbapenemase-producing K. pneumoniae isolated from hospitalized patients in Germany between 2008 and 2014.MethodsWe selected a representative subset of 107 carbapenemase-producing K. pneumoniae clinical isolates possessing the four most prevalent carbapenemase types in Germany (KPC-2, KPC-3, OXA-48, NDM-1). Isolates were processed via illumina NGS. Data were analysed using different SNP-based mapping and de-novo assembly approaches. Relevant information was extracted from NGS data (antibiotic resistance determinants, wzi gene/cps type, virulence genes). NGS data from the present study were also compared with 238 genome data from two previous international studies on K. pneumoniae.ResultsNGS-based analyses revealed a preferred prevalence of KPC-2-producing ST258 and KPC-3-producing ST512 isolates. OXA-48, being the most prevalent carbapenemase type in Germany, was associated with various K. pneumoniae strain types; most of them possessing IncL/M plasmid replicons suggesting a preferred dissemination of blaOXA-48 via this well-known plasmid type. Clusters ST15, ST147, ST258, and ST512 demonstrated an intermingled subset structure consisting of German and other European K. pneumoniae isolates. ST23 being the most frequent MLST type in Asia was found only once in Germany. This latter isolate contained an almost complete set of virulence genes and a K1 capsule suggesting occurrence of a hypervirulent ST23 strain producing OXA-48 in Germany.ConclusionsOur study results suggest prevalence of “classical” K. pneumonaie strain types associated with widely distributed carbapenemase genes such as ST258/KPC-2 or ST512/KPC-3 also in Germany. The finding of a supposed hypervirulent and OXA-48-producing ST23 K. pneumoniae isolates outside Asia is highly worrisome and requires intense molecular surveillance.Electronic supplementary materialThe online version of this article (10.1186/s13756-018-0352-y) contains supplementary material, which is available to authorized users.

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“…or "Please use antibiotics sparingly and only when prescribed by a physician" [7]. Striking images could include a picture of a particularly mucoid stain of Klebsiella pneumoniae [8], or a generic picture of a sick patient lying in a hospital bed. In order to increase their impact, these images and warnings should be adapted to fit with local languages, ethnicities and cultures.…”

Section: Antibiotics As Cigarettesmentioning

confidence: 99%

‘Out-of-the-Box’ Thinking to help Stop the Spread of Antibiotic Resistance

Hays

2016

Future Microbiol.

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String test of hypervirulent Klebsiella pneumonia

Cited by 19 publications

References 1 publication

Biofilm formation and antibiotic resistance inKlebsiella pneumoniaeurinary strains

Biofilm formation and antibiotic resistance inKlebsiella pneumoniaeurinary strains

Genome-based analysis of Carbapenemase-producing Klebsiella pneumoniae isolates from German hospital patients, 2008-2014

‘Out-of-the-Box’ Thinking to help Stop the Spread of Antibiotic Resistance

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