Strigolactone signaling inhibition increases adventitious shoot formation on internodal segments of ipecac

Okazaki, Karin; Watanabe, Setsuko; Koike, Imari; Kawada, Kojiro; Ito, Shinsaku; Nakamura, Hidemitsu; Asami, Tadao; Shimomura, Koichiro; Umehara, Mikihisa

doi:10.1007/s00425-021-03640-1

Cited by 9 publications

(5 citation statements)

References 37 publications

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“…Elevation of CYP711A expression in rice produced SLs, which induced CK degradation by CKX9 [ 37 ]. Our recent study showed that exogenously applied SL suppressed adventitious shoot formation of ipecac, whereas application of SL biosynthesis inhibitors (TIS108 and KK5) or an antagonist (KK094) stimulated it by inducing biosynthesis of 2iP-type CKs [ 38 ]. Because TIS108 and KK5 both inhibited enzymatic activity of CYP711A [ 39 , 40 ], reduction of SL levels might downregulate CKX expression, allowing endogenous CK levels to increase and thus stimulating adventitious shoot formation.…”

Section: Discussionmentioning

confidence: 99%

“…We propose that CK biosynthesis, acquisition of cellular pluripotency, and initiation of cell division spontaneously start at almost the same time following wounding, and elevated endogenous CKs induce adventitious shoot formation without callusing on internodal segments. When we compare endogenous phytohormone levels in some plant species, auxin levels are lower and CK levels are higher in ipecac than in Arabidopsis, rapeseed, and tomato, which cannot form adventitious shoots without phytohormone treatment [ 18 , 38 , 59 , 60 ]. Thus, endogenous CKs (especially 2iP-type CK) is a key regulator of adventitious shoot formation of ipecac.…”

Section: Discussionmentioning

confidence: 99%

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Gene expression profiling before and after internode culture for adventitious shoot formation in ipecac

Okazaki

Koike²,

Kera³

et al. 2022

BMC Plant Biol

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Background In ipecac (Carapichea ipecacuanha (Brot.) L. Andersson), adventitious shoots can be induced simply by placing internodal segments on phytohormone-free culture medium. The shoots form locally on the epidermis of the apical region of the segments, but not the basal region. Levels of endogenous auxin and cytokinin transiently increase in the segments after 1 week of culture. Results Here, we conducted RNA-seq analysis to compare gene expression patterns in apical and basal regions of segments before culture and after 1 week of culture for adventitious shoot formation. The results revealed 8987 differentially expressed genes in a de novo assembly of 76,684 genes. Among them, 276 genes were upregulated in the apical region after 1 week of culture relative to before culture and the basal region after 1 week of culture. These genes include 18 phytohormone-response genes and shoot-formation-related genes. Validation of the gene expression by quantitative real-time PCR assay confirmed that the expression patterns were similar to those of the RNA-seq data. Conclusions The transcriptome data show that expression of cytokinin biosynthesis genes is induced along with the acquisition of cellular pluripotency and the initiation of cell division by wounding in the apical region of internodal segments, that trigger adventitious shoot formation without callusing.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Gene expression profiling before and after internode culture for adventitious shoot formation in ipecac

Okazaki

Koike²,

Kera³

et al. 2022

BMC Plant Biol

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“…Okazaki et al found that KK094 promotes the formation of adventitious shoots of the medicinal plant ipecac. 76 ) This suggests that KK094 may be a plant growth regulator applicable to various plant species. However, no studies have shown that SL receptor inhibitors promote branching and increase yields at the field level.…”

Section: Receptor Inhibition In Parasitic Plantsmentioning

confidence: 99%

Emerging technologies for the chemical control of root parasitic weeds

et al. 2022

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Parasitic plants in the Orobanchaceae family include devastating weed species, such as Striga, Orobanche, and Phelipanche, which parasitize major crops, drastically reduces crop yields and cause economic losses of over a billion US dollars worldwide. Advances in basic research on molecular and cellular processes responsible for parasitic relationships has now achieved steady progress through advances in genome analysis, biochemical analysis and structural biology. On the basis of these advances it is now possible to develop chemicals that control parasitism and reduce agricultural damage. In this review we summarized the recent development of chemicals that can control each step of parasitism from strigolactone biosynthesis in host plants to haustorium formation.

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“…Notably, auxin and ethylene both induce root hair elongation, and SLs may function upstream of the ethylene signaling pathway [ 83 ]. In addition, adventitious rooting was enhanced in SLs-signaling mutants of Arabidopsis and ipecac ( Carapichea ipecacuanha ), implying that SLs inhibit adventitious rooting [ 81 , 156 ].…”

Section: Altered Sls Metabolism Through Gene Manipulation To Cope With Environmental Stressesmentioning

confidence: 99%

Harmony but Not Uniformity: Role of Strigolactone in Plants

Rehman

Zeng

et al. 2021

Biomolecules

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Strigolactones (SLs) represent an important new plant hormone class marked by their multifunctional roles in plants and rhizosphere interactions, which stimulate hyphal branching in arbuscular mycorrhizal fungi (AMF) and seed germination of root parasitic plants. SLs have been broadly implicated in regulating root growth, shoot architecture, leaf senescence, nodulation, and legume–symbionts interaction, as well as a response to various external stimuli, such as abiotic and biotic stresses. These functional properties of SLs enable the genetic engineering of crop plants to improve crop yield and productivity. In this review, the conservation and divergence of SL pathways and its biological processes in multiple plant species have been extensively discussed with a particular emphasis on its interactions with other different phytohormones. These interactions may shed further light on the regulatory networks underlying plant growth, development, and stress responses, ultimately providing certain strategies for promoting crop yield and productivity with the challenges of global climate and environmental changes.

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Strigolactone signaling inhibition increases adventitious shoot formation on internodal segments of ipecac

Cited by 9 publications

References 37 publications

Gene expression profiling before and after internode culture for adventitious shoot formation in ipecac

Gene expression profiling before and after internode culture for adventitious shoot formation in ipecac

Emerging technologies for the chemical control of root parasitic weeds

Harmony but Not Uniformity: Role of Strigolactone in Plants

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