Stretching stimulates fibulin‐5 expression and controls microfibril bundles in human periodontal ligament cells

Nakashima, Kazuki; Tsuruga, Eichi; Hisanaga, Yutaka; Ishikawa, Hiroyuki; Sawa, Yoshihiko

doi:10.1111/j.1600-0765.2008.01170.x

Cited by 27 publications

(16 citation statements)

References 17 publications

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“…We showed previously that fibulin-5 localizes with oxytalan fibers to control their coalescence [16]. In the present study, we showed by immunoprecipitation that EMILIN-1 colocalizes with fibrillin-1.…”

Section: Discussionsupporting

confidence: 71%

“…An ultrastructural study of EMILIN-1 immunolocalization revealed that the molecule was clearly labeled in oxytalan fibers of the corneal stroma [13]. Moreover, based on the fact that EMILIN-1 can bind with fibulin-5, which is expressed on oxytalan fibers in our PDL culture model [15,16], we hypothesized that EMILIN-1 might contribute to the formation of oxytalan fibers in PDL. In the present study, we used a culture model of pure microfibrils to investigate the role of EMILIN-1 in the development of oxytalan fibers and found that EMILIN-1 colocalizes with oxytalan fibers and regulates their biogenesis.…”

Section: Introductionmentioning

confidence: 89%

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EMILIN-1 regulates the amount of oxytalan fiber formation in periodontal ligaments in vitro

Nakatomi

Tsuruga

Nakashima

et al. 2010

Connective Tissue Research

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The elastic system fibers comprise oxytalan, elaunin, and elastic fibers, differing in their relative microfibril and elastin contents. Among them, human periodontal ligament (PDL) contains only oxytalan fibers (pure microfibrils). Elastin microfibril interface-located protein-1 (EMILIN-1) is localized at the interface between microfibrils and elastin. We hypothesized that EMILIN-1 may contribute to the formation of oxytalan fibers. We used a small interfering RNA (siRNA) for EMILIN-1 in PDL cell culture to examine the extracellular deposition of fibrillin-1 (the major component of microfibrils). EMILIN-1 was labeled on microfibrils positive for fibrillin-1 and was colocalized with fibrillin-1 upon immunoprecipitation assay. EMILIN-1 suppression reduced the level of fibrillin-1 deposition to 23% of the control, and this was responsible for the diminution of fibrillin-1 deposition revealed by immunofluorescence. These results suggest that EMILIN-1 may regulate the formation of oxytalan fibers and play a role in their homeostasis.

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Section: Discussionsupporting

confidence: 71%

Section: Introductionmentioning

confidence: 89%

EMILIN-1 regulates the amount of oxytalan fiber formation in periodontal ligaments in vitro

Nakatomi

Tsuruga

Nakashima

et al. 2010

Connective Tissue Research

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“…Functional strain has been shown to induce coalescence of oxytalan fibers in cultured PDL fibroblasts [9]. By means of the gene silencing technique, we have demonstrated that fibulin-5, upregulated upon exposure to stretching stress, is a key molecule for the induction of oxytalan fiber coalescence [10]. Fibulin-5 is also known to be a crucial molecule in the control of elastic fiber formation [11,12].…”

Section: Introductionmentioning

confidence: 94%

“…PDL fibroblasts were isolated from three different donors and cultured, as described previously [10]. Briefly, connective tissues were obtained surgically from the PDL of premolar teeth extracted for orthodontic reasons.…”

Section: Cells and Culturementioning

confidence: 99%

Latent Transforming Growth Factor-β Binding Protein 2 Negatively Regulates Coalescence of Oxytalan Fibers Induced by Stretching Stress

Tsuruga¹,

Oka

Hatakeyama³

et al. 2012

Connective Tissue Research

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Oxytalan fibers are extracellular matrix components consisting of pure microfibrils. However, the mechanism whereby oxytalan fibers develop is not fully understood. We have previously reported that in human periodontal ligament (PDL) fibroblasts subjected to stretching stress, bundles of oxytalan fibers coalesce under the control of fibulin-5. Latent transforming growth factor-β binding protein 2 (LTBP-2) is known to bind to fibulin-5. The purpose of this study was to clarify the role of LTBP-2 in the coalescence of oxytalan fibers. We subjected PDL fibroblasts to stretching in order to examine the effects of LTBP-2 on the coalescence of oxytalan fibers in cell/matrix layers. Interaction of LTBP-2 with fibulin-5 was examined by immunoprecipitation assay, and changes in LTBP-2 deposition upon stretching were investigated by Western blotting and immunofluorescence assays. We used small interfering RNA against LTBP-2 in PDL cell culture and examined the appearance of oxytalan fibers on the basis of immunofluorescence. Stretching induced coalescence of oxytalan fibers, but did not affect LTBP-2 expression. The amount of extracellularly deposited LTBP-2 was decreased by about 70% as a result of stretching, compared with the control. LTBP-2 interacted with fibulin-5 on the fibers, and stretching decreased the amount of the LTBP-2 interacted with fibulin-5 by about 60%. Oxytalan fiber coalescence did not occur when LTBP-2 was suppressed by about 95%, whereas it occurred when LTBP-2 was suppressed by about 40%, fibulin-5 being colocalized with oxytalan fibers. These results suggest that LTBP-2, in response to tension stress, may negatively control the function of fibulin-5, thereby modulating the mechanism of oxytalan fiber coalescence.

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“…PDL cell culture and migration assay PDL fibroblasts were isolated from a donor and cultured, as described previously 23) . Culture medium for PDL fibroblasts was supplemented with 10 % newborn calf serum (NCS; Invitrogen, Carlsbad, CA, USA) and 100 units/ml penicillin and 100 µg/ml streptomycin (Roche Diagnostics, Mannheim, Germany) at 37 °C in humidified air containing 5 % CO 2 .…”

Section: Section Preparation and Immunohistochemistrymentioning

confidence: 99%

Effects of Fibrillin Application on Periodontal Ligament Regeneration in Mouse Model of Tooth Replantation

Tamura

Oka

Itaya

et al. 2016

J. Hard Tissue Biology.

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The periodontal ligament (PDL) is fibrous tissue that maintains the connective space between tooth root and alveolar bone while avoiding the root resorption and the ankylosis. Fibrillin is one of the components of the elastic system fibers which exist in PDL as oxytalan fibers, and it plays an important role in organizing PDL with collagen fibers. The aim of the present study was to evaluate the effect of applying fibrillin protein for PDL regeneration using a mouse tooth replantation model. The ratio of root resorption and ankylosis was not improved in a replanted tooth soaked in fibrillin/PBS solution as compared to being soaked in PBS only. Interestingly, replantation with fibrillin and propylene glycol alginate (fibrillin/PGA) induced less root resorption and ankylosis than were observed with teeth replanted with PGA only. The PDL space between tooth root and alveolar bone in the fibrillin/PGA was clearly wider than that with PGA only. This regenerated PDL with Azan staining showed the meshwork structures including rich fibers in the horizontal direction. Furthermore, immunohistochemical expression of periostin was strongly detected in the entire regenerated PDL in the fibrillin/ PGA. These results suggest that fibrillin promotes the formation of fibrous connective tissue in a PDL replantation and might be a good candidate as regenerative material for the PDL.

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Stretching stimulates fibulin‐5 expression and controls microfibril bundles in human periodontal ligament cells

Abstract: These results suggest that fibulin-5 up-regulated in response to tension strain may control the formation of microfibril bundles in periodontal ligament.

Cited by 27 publications

References 17 publications

EMILIN-1 regulates the amount of oxytalan fiber formation in periodontal ligaments in vitro

EMILIN-1 regulates the amount of oxytalan fiber formation in periodontal ligaments in vitro

Latent Transforming Growth Factor-β Binding Protein 2 Negatively Regulates Coalescence of Oxytalan Fibers Induced by Stretching Stress

Effects of Fibrillin Application on Periodontal Ligament Regeneration in Mouse Model of Tooth Replantation

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