Stretching of Single Dna Molecules by Langmuir–blodgett Method

Abstract

We propose a new method that double-stranded DNA molecules can be stretched and immobilized on the clean glass substrate by using a lipid monolayer at the air-water interface. This method is based on the substrate lifting of Langmuir-Blodgett method. We observed fluorescence images of polyion complex films with a scanning near-field optical microscope (SNOM). As a result, straight fluorescent lines aligned parallel to the lifting direction were observed and it was considered that isolated single DNA molecules were extended to align on the substrate. This method is applied to various DNA molecules.

Sequence-specifically platinum metal deposition on enzymatically synthesized DNA block copolymer

“…0.50 nm). 23 The length of segment B (1.5 mm) accorded well with the length of the ATpart estimated from the extension rate of d(AT)10 templateprimers. These results indicate that segment B corresponded to the AT-part in the DNA block copolymer.…”

“…4 shows an AFM image and height histogram of the platinum deposited DNA block copolymer stretched and immobilized on a mica substrate by the LB method. 11,23,24 We produced the height histograms from cross-sectional images of the AFM image shown in Fig. 4(a).…”

“…The stretches of DNA ending in tails which appears significantly higher than the rest is caused by folding of DNA ending in the process of the DNA transferring onto a substrate. 23 Accordingly, segment A could be identified as the GC-part in the DNA block copolymer. This indicated the successful enzymatic synthesis of the DNA block copolymer and its sequence-selective modification of 1-2 nm platinum clusters.…”

Fabrication of Metal Nanowires by Electroless Plating of DNA

“…We have found that when a DNA-amphiphile polyion complex monolayer, which is formed at the air-water interface, was transferred to a glass substrate using the Langmuir-Blodgett (LB) method, DNA molecules could be immobilized on the glass substrate in a stretched configuration [10,11]. The stretching direction of the DNA molecules is parallel to the lifting direction of the substrate.…”