Stretchable Piezoelectric Substrate Providing Pulsatile Mechanoelectric Cues for Cardiomyogenic Differentiation of Mesenchymal Stem Cells

Yoon, Jong Il; Lee, Tae Il; Bhang, Suk Ho; Shin, Jung Youn; Myoung, Jae Min; Kim, Byung Soo

doi:10.1021/acsami.7b03050

Cited by 22 publications

(19 citation statements)

References 59 publications

(114 reference statements)

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“…demonstrated a contractile cardiomyocyte‐driven PVDF piezoelectric nanofiber as a biogenerator, which generated an output of 200 mV (open‐circuit voltage) and 45 nA (short‐circuit current). [ 289 ] Piezoelectric PLGA/BTO, [ 290 ] electrospun P(VDF‐TrFE), [ 161a ,293] PCL/P(VDF‐TrFE), [ 292 ] ZnO nanorods/PDMS [ 293 ] have also been explored for promoting CMs proliferation, alignment, contraction, cell‐cell communication, and myocardial differentiation. Not only that, piezoelectric scaffolds can also monitor contractile activity of CMs and heart based on the voltage transients, simultaneously.…”

Section: Application In Tissue Regenerationmentioning

confidence: 99%

Electroactive Biomaterials and Systems for Cell Fate Determination and Tissue Regeneration: Design and Applications

et al. 2021

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During natural tissue regeneration, tissue microenvironment and stem cell niche including cell–cell interaction, soluble factors, and extracellular matrix (ECM) provide a train of biochemical and biophysical cues for modulation of cell behaviors and tissue functions. Design of functional biomaterials to mimic the tissue/cell microenvironment have great potentials for tissue regeneration applications. Recently, electroactive biomaterials have drawn increasing attentions not only as scaffolds for cell adhesion and structural support, but also as modulators to regulate cell/tissue behaviors and function, especially for electrically excitable cells and tissues. More importantly, electrostimulation can further modulate a myriad of biological processes, from cell cycle, migration, proliferation and differentiation to neural conduction, muscle contraction, embryogenesis, and tissue regeneration. In this review, endogenous bioelectricity and piezoelectricity are introduced. Then, design rationale of electroactive biomaterials is discussed for imitating dynamic cell microenvironment, as well as their mediated electrostimulation and the applying pathways. Recent advances in electroactive biomaterials are systematically overviewed for modulation of stem cell fate and tissue regeneration, mainly including nerve regeneration, bone tissue engineering, and cardiac tissue engineering. Finally, the significance for simulating the native tissue microenvironment is emphasized and the open challenges and future perspectives of electroactive biomaterials are concluded.

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Section: Application In Tissue Regenerationmentioning

confidence: 99%

Electroactive Biomaterials and Systems for Cell Fate Determination and Tissue Regeneration: Design and Applications

et al. 2021

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“…This is the reason why cell cultures in vitro and tissue-engineered samples tend to reproduce the correct environmental conditions that real cells would live in (Costa et al 2012). Consequently, just to mention two relevant examples, muscle cells and cardiac cells are often grown on substrata that are cyclically stretched, in order to mimic respectively muscle contraction and heartbeat (Kim et al 1999;Laflamme and Murry 2011;Yoon et al 2017). Now, when subject to a periodic deformation, several types of cells (not only those that have a bipolar morphology, such as fibroblasts, myofibroblasts, myocytes, airway smooth muscle cells, but also endothelial cells (Moretti et al 2004)) show a peculiar response that proves their subtle sensitivity to mechanical prompts: when laying on a substratum they tend to re-orient themselves until they reach a stable configuration, with a well-defined angle between their polarization axis (along which the stress fibers become mainly aligned) and the direction of stretching.…”

Section: Introductionmentioning

confidence: 99%

A nonlinear elastic description of cell preferential orientations over a stretched substrate

Lucci

Preziosi

2021

Biomech Model Mechanobiol

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The active response of cells to mechanical cues due to their interaction with the environment has been of increasing interest, since it is involved in many physiological phenomena, pathologies, and in tissue engineering. In particular, several experiments have shown that, if a substrate with overlying cells is cyclically stretched, they will reorient to reach a well-defined angle between their major axis and the main stretching direction. Recent experimental findings, also supported by a linear elastic model, indicated that the minimization of an elastic energy might drive this reorientation process. Motivated by the fact that a similar behaviour is observed even for high strains, in this paper we address the problem in the framework of finite elasticity, in order to study the presence of nonlinear effects. We find that, for a very large class of constitutive orthotropic models and with very general assumptions, there is a single linear relationship between a parameter describing the biaxial deformation and $$\cos ^2\theta _{\mathrm{eq}}$$ cos 2 θ eq , where $$\theta _{\mathrm{eq}}$$ θ eq is the orientation angle of the cell, with the slope of the line depending on a specific combination of four parameters that characterize the nonlinear constitutive equation. We also study the effect of introducing a further dependence of the energy on the anisotropic invariants related to the square of the Cauchy–Green strain tensor. This leads to departures from the linear relationship mentioned above, that are again critically compared with experimental data.

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“…These biomaterials can convey the mechanical force generated by the dynamic deformation to the adherent cells and adapt in real‐time to fit the changing mechanical properties of tissues at the implanted site. Frequently used stimulations include magnets, [ 72b ] electricity, [ 76 ] temperature, [ 77 ] light, [ 78 ] and ion. [ 79 ] Hydrogels are one of the most commonly used biomaterials to construct stimuli‐responsive deformable actuators owing to their high water content, which is comparable to that in biological tissues, noncytotoxicity, and mechanical properties consistent with those of target tissues that can be tailored by changing the degree of crosslinking.…”

Section: Biophysical Cues Of Biomaterials For Regulating Stem Cell Behaviormentioning

confidence: 99%

“…This effect can provide mechanoelectrical cues for cardiomyogenic differentiation of MSCs. [ 76 ] Our group recently reported that piezoelectric polyvinylidene fluoride (PVDF) films with aligned nanostripes can promote neuron‐like differentiation of MSCs. Compared to the nonpiezoelectric control with the same surface topography, the traction force of MSCs can induce the surface piezoelectric potential of the piezoelectric topography, which in turn affected the cells to substantially increase neuron‐like differentiation.…”

Section: Biophysical Cues Of Biomaterials For Regulating Stem Cell Behaviormentioning

confidence: 99%

Manipulation of Stem Cells Fates: The Master and Multifaceted Roles of Biophysical Cues of Biomaterials

Wan

Liu

2021

Adv Funct Materials

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Owing to their self‐renewal and differentiation ability, stem cells are conducive for repairing injured tissues, making them a promising source of seed cells for tissue engineering. The extracellular microenvironment (ECM) is under dynamic mechanical control, which is closely related to stem cell behaviors. During the design and fabrication of biomaterials for regenerative medicine, the physiochemical properties of the natural ECM should be closely mimicked, which can reinforce stem cell lineage choice and tissue engineering. By reproducing the biophysical stimulations that stem cells may experience in vivo, many studies have highlighted the key role of biophysical cues in regulation of cell fate. Optimization of biophysical factors leads to desirable stem cell functions, which can maximize the effectiveness of regenerative treatment. In this review, the main biophysical cues of biomaterials, including stiffness, topography, mechanical force, and external physical fields are summarized, and their individual and synergistic influence on stem cell behavior is discussed. Subsequently, the current progress in tissue regeneration using biomaterials is presented, which directs the design and fabrication of functional biomaterial. The mechanisms via which biophysical cues activate cellular responses are also analyzed. Finally, the challenges in basic research as well as for clinical translation in this field are discussed.

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Stretchable Piezoelectric Substrate Providing Pulsatile Mechanoelectric Cues for Cardiomyogenic Differentiation of Mesenchymal Stem Cells

Cited by 22 publications

References 59 publications

Electroactive Biomaterials and Systems for Cell Fate Determination and Tissue Regeneration: Design and Applications

Electroactive Biomaterials and Systems for Cell Fate Determination and Tissue Regeneration: Design and Applications

A nonlinear elastic description of cell preferential orientations over a stretched substrate

Manipulation of Stem Cells Fates: The Master and Multifaceted Roles of Biophysical Cues of Biomaterials

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