Stretch-Induced Tenomodulin Expression Promotes Tenocyte Migration via F-Actin and Chromatin Remodeling

Xu, Pu; Deng, Bin; Zhang, Bingyu; Luo, Qing; Song, Guanbin

doi:10.3390/ijms22094928

Cited by 11 publications

(5 citation statements)

References 31 publications

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“…Previously, we determined that stress deprivation decreases the proportion of F-actin in tendon as compared to freshly isolated tendon. Stretching isolated tenocytes in culture remodels F-actin in order to elicit alterations in gene expression [ 29 ]. Therefore, we sought to determine the effect of mechanical restraining on tenocyte F-actin within native tendons.…”

Section: Resultsmentioning

confidence: 99%

The application of mechanical load onto mouse tendons by magnetic restraining represses Mmp-3 expression

et al. 2023

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Objectives Mechanical loading is crucial for tendon matrix homeostasis. Under-stimulation of tendon tissue promotes matrix degradation and ultimately tendon failure. In this study, we examined the expression of tendon matrix molecules and matrix-degrading enzymes (matrix metalloproteinases) in stress-deprived tail tendons and compared to tendons that were mechanically loaded by a simple restraining method. Data description Isolated mouse tail fascicles were either floated or restrained by magnets in cell culture media for 24 h. The gene expression of tendon matrix molecules and matrix metalloproteinases in the tendon fascicles of mouse tails were examined by real-time RT-PCR. Stress deprivation of tail tendons increase Mmp3 mRNA levels. Restraining tendons represses these increases in Mmp3. The gene expression response to restraining was specific to Mmp3 at 24 h as we did not observe mRNA level changes in other matrix related genes that we examined (Col1, Col3, Tnc, Acan, and Mmp13). To elucidate, the mechanisms that may regulate load transmission in tendon tissue, we examined filamentous (F-)actin staining and nuclear morphology. As compared to stress deprived tendons, restrained tendons had greater staining for F-actin. The nuclei of restrained tendons are smaller and more elongated. These results indicate that mechanical loading regulates specific gene expression potentially through F-actin regulation of nuclear morphology. A further understanding on the mechanisms involved in regulating Mmp3 gene expression may lead to new strategies to prevent tendon degeneration.

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Section: Resultsmentioning

confidence: 99%

The application of mechanical load onto mouse tendons by magnetic restraining represses Mmp-3 expression

et al. 2023

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“…This study found more matrix-related TNMD expression in the 3D-aligned TSPC group for the in vitro and in vivo experiments. It has been shown that the expression of TNMD is upregulated in tendon cells when mechanically stimulated and cultured on the aligned scaffold. − The 3D-aligned TSPCs played an important role in assisting the injured tendons in getting excessive inflammation. Recent studies have revealed that aligned nanofibers induce the development of a pro-healing M2 phenotype .…”

Section: Discussionmentioning

confidence: 99%

Sandwich Biomimetic Scaffold Based Tendon Stem/Progenitor Cell Alignment in a 3D Microenvironment for Functional Tendon Regeneration

Sun

Chen

et al. 2023

ACS Appl. Mater. Interfaces

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Tendon injuries are some of the most commonly diagnosed musculoskeletal diseases. Tendon regeneration is sensitive to the topology of the substitute as it affects the cellular microenvironment and homeostasis. To bionic in vivo three-dimensional (3D) aligned microenvironment, an ordered 3D sandwich model was used to investigate the cell response in the tendon. First, high-resolution 3D printing provided parallel-grooved topographical cues on the hydrogel surface. Then the cells were seeded on its surface to acquire a 2D model. Afterward, an additional hydrogel coating layer was applied to the cells to create the 3D model. The interaction between cells and order structures in three-dimensions is yet to be explored. The study found that the tendon stem/progenitor cells (TSPCs) still maintain their ordering growth in the 3D model as in the 2D model. The study also found that the 3D-aligned TSPCs exhibited enhanced tenogenic differentiation through the PI3K-AKT signaling pathway and presented a less inflammatory phenotype than those in the 2D model. The in vivo implantation of such a 3D-aligned TSPC composite promoted tendon regeneration and mitigated heterotopic ossification in an Achilles defect model. These findings demonstrated that 3D-aligned TSPCs within a biomimetic topology environment are promising for functional tendon regeneration.

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“…The proliferation and migration of tenocytes are believed to play an important role in tendon healing [52] and are related to the strength of the healed tendons [53][54][55][56]. In our previous study, human tenocytes treated with polysaccharides extracted from Bletilla striata demonstrated better migration and enhanced proliferation via activation of MEK/ERK1/2 and PI3K/Akt signaling pathways, as well as in the secretion of extracellular matrix [57].…”

Section: Evs Have Been Generally Accepted As a Modality Of Cell-to-cell Communication Formentioning

confidence: 97%

Extracellular Vesicles of Adipose-Derived Stem Cells Promote the Healing of Traumatized Achilles Tendons

Chen

Lin

et al. 2021

IJMS

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Healing of ruptured tendons remains a clinical challenge because of its slow progress and relatively weak mechanical force at an early stage. Extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) have therapeutic potential for tissue regeneration. In this study, we isolated EVs from adipose-derived stem cells (ADSCs) and evaluated their ability to promote tendon regeneration. Our results indicated that ADSC-EVs significantly enhanced the proliferation and migration of tenocytes in vitro. To further study the roles of ADSC-EVs in tendon regeneration, ADSC-EVs were used in Achilles tendon repair in rabbits. The mechanical strength, histology, and protein expression in the injured tendon tissues significantly improved 4 weeks after ADSC-EV treatment. Decorin and biglycan were significantly upregulated in comparison to the untreated controls. In summary, ADSC-EVs stimulated the proliferation and migration of tenocytes and improved the mechanical strength of repaired tendons, suggesting that ADSC-EV treatment is a potential highly potent therapeutic strategy for tendon injuries.

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Stretch-Induced Tenomodulin Expression Promotes Tenocyte Migration via F-Actin and Chromatin Remodeling

Cited by 11 publications

References 31 publications

The application of mechanical load onto mouse tendons by magnetic restraining represses Mmp-3 expression

The application of mechanical load onto mouse tendons by magnetic restraining represses Mmp-3 expression

Sandwich Biomimetic Scaffold Based Tendon Stem/Progenitor Cell Alignment in a 3D Microenvironment for Functional Tendon Regeneration

Extracellular Vesicles of Adipose-Derived Stem Cells Promote the Healing of Traumatized Achilles Tendons

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