'Stressing' rodent self-grooming for neuroscience research

Song, Cai; Berridge, Kent; Kalueff, Allan V.

doi:10.1038/nrn.2016.103

Cited by 49 publications

(34 citation statements)

References 15 publications

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“…7a). In line, the grooming behavior, indicating the level of stress to which rodents might be exposed (Song et al, 2016), it was significantly reduced in all experimental groups in comparison to control ( Supplementary Fig. 7b).…”

Section: Behavioral Correlates Of General De-and Remyelinationmentioning

confidence: 59%

Myelination- and immune mediated MR-based brain network correlates

Cerina

Gallus

Koirala³

et al. 2020

Preprint

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Background: Multiple sclerosis (MS) is an autoimmune disease of the central nervous system (CNS), characterized by inflammatory and neurodegenerative processes. Despite demyelination being a hallmark of the disease, how it relates to neurodegeneration has still not been completely unraveled, and research is still ongoing into how these processes can be tracked non-invasively. Magnetic resonance imaging (MRI) derived brain network characteristics, which closely mirror disease processes and relate to functional impairment, recently became important variables for characterizing immune-mediated neurodegeneration, however their histopathological basis remains unclear. Methods: In order to determine the MRI-derived correlates of myelin dynamics and to test if brain network characteristics derived from diffusion tensor imaging reflect microstructural tissue reorganization, we took advantage of the cuprizone model of general demyelination in mice and performed longitudinal histological and imaging analyses with behavioral tests. By introducing cuprizone into the diet, we induced targeted and consistent demyelination of oligodendrocytes, over a period of 5 weeks. Subsequent myelin synthesis was enabled by reintroduction of normal food. Results: Using specific immune-histological markers, we demonstrated that two weeks of cuprizone diet induced a 52% reduction of myelin content in the corpus callosum (CC) and a 35% reduction in the neocortex. An extended cuprizone diet increased myelin loss in the CC, while remyelination commenced in the neocortex. These histologically determined dynamics were reflected by MRI measurements from diffusion tensor imaging. Demyelination was associated with decreased fractional anisotropy (FA) values and increased modularity and clustering at the network level. MRI-derived modularization of the brain network and FA reduction in key anatomical regions, including the hippocampus, thalamus and analyzed cortical areas, were closely related to impaired memory function and anxiety-like behavior. Conclusion: Network specific remyelination, shown by histology and MRI metrics, determined amelioration of functional performance and neuropsychiatric symptoms. Taken together, we illustrate the histological basis for the MRI-driven network responses to demyelination, where increased modularity leads to evolving damage and abnormal behavior in MS. Quantitative information about in vivo myelination processes is mirrored by diffusion based imaging of microstructural integrity and network characteristics.

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Section: Behavioral Correlates Of General De-and Remyelinationmentioning

confidence: 59%

Myelination- and immune mediated MR-based brain network correlates

Cerina

Gallus

Koirala³

et al. 2020

Preprint

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“…However, analysis of distinct grooming types revealed specific excessive grooming of the back in Fmr1-KO mice exposed to the novel open field, a phenotype reversed by pretreatment with BMS-204352. Previous work has shown that the behavioral microstructure of self-grooming in rodents may serve as a sensitive marker of stress levels, even without significant change in overall grooming time (Song et al, 2016). Increased caudal selfgrooming has been reported as a response to relatively moderate aversive conditions such as bright light and novelty exposure (Meshalkina and Kalueff, 2016;van Erp et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

Potential Involvement of Impaired BKCa Channel Function in Sensory Defensiveness and Some Behavioral Disturbances Induced by Unfamiliar Environment in a Mouse Model of Fragile X Syndrome

Carreno-Muñoz

Martins

Medrano

et al. 2017

Neuropsychopharmacol.

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In fragile X syndrome (FXS), sensory hypersensitivity and impaired habituation is thought to result in attention overload and various behavioral abnormalities in reaction to the excessive and remanent salience of environment features that would normally be ignored. This phenomenon, termed sensory defensiveness, has been proposed as the potential cause of hyperactivity, hyperarousal, and negative reactions to changes in routine that are often deleterious for FXS patients. However, the lack of tools for manipulating sensory hypersensitivity has not allowed the experimental testing required to evaluate the relevance of this hypothesis. Recent work has shown that BMS-204352, a BK channel agonist, was efficient to reverse cortical hyperexcitability and related sensory hypersensitivity in the Fmr1-KO mouse model of FXS. In the present study, we report that exposing Fmr1-KO mice to novel or unfamiliar environments resulted in multiple behavioral perturbations, such as hyperactivity, impaired nest building and excessive grooming of the back. Reversing sensory hypersensitivity with the BK channel agonist BMS-204352 prevented these behavioral abnormalities in Fmr1-KO mice. These results are in support of the sensory defensiveness hypothesis, and confirm BK as a potentially relevant molecular target for the development of drug medication against FXS/ASD.

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“…In addition, self-grooming can also be an expression of stress. Taking rodent as a research model, previous studies [41][42][43] has reported that the relationship between stress and self-grooming can be described as an inverted U-shaped: Self-grooming typically occurs spontaneously at low stress and becomes longer during moderate stress and can be inhibited by high-stress states. Thus, the higher self-grooming of individually housed calves may respond to the higher stress (moderate stress) they faced compared with paired calves during weaning and postweaning periods.…”

Section: Behaviormentioning

confidence: 99%

Effects of Pair Versus Individual Housing on Performance, Health, and Behavior of Dairy Calves

Лю

et al. 2019

Animals

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The aim of this study was to evaluate the effects of pair versus individual housing on performance, health, and behavior of dairy calves. Thirty female Holstein dairy calves were assigned to individual (n = 10) or pair housing (n = 10 pairs). The results showed that both treatments had a similar starter intake and average daily gain (ADG) during the preweaning period. During weaning and postweaning periods, paired calves had a higher starter intake, and the ADG of paired calves continued to increase but calves housed individually experienced a growth check. Paired calves showed higher diarrhea frequency only in week three. The results on behavior showed that feeding, chewing and ruminating time increased, and self-grooming time decreased with age during weaning and postweaning periods, and paired calves spent less time feeding, standing and self-grooming but more time lying during this time. After mixing, feeding, and chewing and ruminating time continued to rise, and self-grooming time continued to decline for both treatments. All calves spent less time standing and non-nutritive manipulation after mixing, and previously individually housed calves tended to increase non-nutritive manipulation. These results showed that pair housing improved growth during weaning and postweaning periods and that calves altered their behavior at different phases. Less social contact may lead to more non-nutritive manipulation.

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'Stressing' rodent self-grooming for neuroscience research

Cited by 49 publications

References 15 publications

Myelination- and immune mediated MR-based brain network correlates

Myelination- and immune mediated MR-based brain network correlates

Potential Involvement of Impaired BKCa Channel Function in Sensory Defensiveness and Some Behavioral Disturbances Induced by Unfamiliar Environment in a Mouse Model of Fragile X Syndrome

Effects of Pair Versus Individual Housing on Performance, Health, and Behavior of Dairy Calves

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