Stressed Jerusalem artichoke tubers (Helianthus tuberosus L.) excrete a protein fraction with specific cytotoxicity on plant and animal tumour cell

Griffaut, B.; Débiton, Eric; Madelmont, Jean‐Claude; Maurizis, Jean‐Claude; Ledoigt, Gérard

doi:10.1016/j.bbagen.2007.06.007

Cited by 14 publications

(14 citation statements)

References 33 publications

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“…Stressed Jerusalem artichoke tubers excrete proteins causing necrosis of crowngalled tissue and also exhibiting activity against some human tumor cells, especially mammary tumor cells. (43) Antitumoral activity was mainly associated with an 18-kDa superoxide dismutase, but the presence of a 28 kDa protein with a DING N terminus (Table 1 and Fig. 1) also seemed important.…”

Section: More Ding Proteinsmentioning

confidence: 96%

The DING family of proteins: ubiquitous in eukaryotes, but where are the genes?

et al. 2009

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PstS and DING proteins are members of a superfamily of secreted, high-affinity phosphate-binding proteins. Whereas microbial PstS have a well-defined role in phosphate ABC transporters, the physiological function of DING proteins, named after their DINGGG N termini, still needs to be determined. PstS and DING proteins co-exist in some Pseudomonas strains, to which they confer a highly adhesive and virulent phenotype. More than 30 DING proteins have now been purified, mostly from eukaryotes. They are often associated with infections or with dysregulation of cell proliferation. Consequently, eukaryotic DING proteins could also be involved in cell-cell communication or adherence. The ubiquitous presence in eukaryotes of proteins structurally and functionally related to bacterial virulence factors is intriguing, as is the absence of eukaryotic genes encoding DING proteins in databases. DING proteins in eukaryotes could originate from unidentified commensal or symbiotic bacteria and could contribute to essential functions. Alternatively, DING proteins could be encoded by eukaryotic genes sharing special features that prevent their cloning. Both hypotheses are discussed.

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Section: More Ding Proteinsmentioning

confidence: 96%

The DING family of proteins: ubiquitous in eukaryotes, but where are the genes?

et al. 2009

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“…At least one of these compounds, kinetin riboside, induced a cytotoxic effect both on plant and mammalian tumour cells [1]. In Jerusalem artichoke tubers (Helianthus tuberosus), after the same stimuli, we have shown secretion of a cytotoxic protein related to a superoxide dismutase [2]. This protein alone or possibly associated with a co-factor was shown to induce cytotoxicity in plant and animal tumour cells, preserving non-tumour cells in both cases.…”

Section: Introductionmentioning

confidence: 77%

“…In contrast, BEL, which specifically inhibits iPLA 2 activity, did not significantly decrease lipid acyl hydrolase activity of the product, and addition of EGTA, a calcium chelator which inhibits cPLA 2 activity, also did not decrease acyl hydrolase activity. Therefore, secreted patatin-like protein displayed unusual features for PLA 2 activity, and could be classed as BEL-insensitive iPLA 2 .…”

Section: Discussionmentioning

confidence: 99%

“…Secretion of cytotoxic products against melanoma B16 cells, thus, can be performed through specific stress on storage organs of plants usually cultivated [93]. In Jerusalem artichoke (Helianthus tuberosus L.) tubers, active agent was shown to contain 18-kDa polypeptide with homology to superoxide dismutase (SOD) [2].…”

Section: Discussionmentioning

confidence: 99%

“…Apoptotic cell death induction through several cancer cell death mechanisms, including activation of caspase and matrix metalloproteinases, is important for melittin-induced anti-cancer effects [84]. Loss of cPLA 2 activity, either through genetic knockout in mice, or by treatment with a cPLA 2 inhibitor, results in an attenuation of arachidonic acid release as well as of the apoptotic response to oxLDL in peritoneal macrophages or to 25-OHC in cultured fibroblast and macrophage cell lines [85].…”

Section: Discussionmentioning

confidence: 99%

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Patatin-like PLA<sub>2</sub> with cytotoxicity against mammalian and plant tumour cells

Griffaut¹,

Débiton²,

Galmier³

et al. 2013

ABC

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Plants can respond to traumatism by synthesis and secretion of defence molecules. Wound-healing and desiccation stress of pieces of potato tuber parenchyma (Solanum tuberosum) promoted the secretion of a patatin-like phospholipase A 2 , PLA 2 (EC.3.1.1.4.) that displayed cytotoxic activity against tumour cells. The potato secretion product, an oligomeric form of patatin-like protein, was shown to contain several isoforms of PLA 2 polypeptides and to be associated with other proteins, including Kunitz-type protease inhibitors. Patatin-like protein secretion was inhibited by vanadate. Secreted patatin-like proteins displayed specific features, such as extracellular function and low molecular weights, mainly 36 to 40 kDa. The 36-kDa polypeptide sequence was related to iPLA 2 α. Polypeptide spots of secreted patatin-like protein exhibited a nucleotide-binding consensus motif, GGGIKG that has been described in iPLA 2 gene family. The cytotoxic agent caused cell death of plant crown-gall induced by Agrobacterium tumefaciens, and inhibited B16 cell proliferation, but at the same concentration did not display any toxicity against non-transformed cells. PLA 2 enzyme activity was required for cytotoxicity against B16 melanoma cells. A model for such a specific activity against tumour cells is discussed in connection with asymmetric phospholipid patterns of cell membranes. In conclusion, secreted patatin-like PLA 2 (e-patatin) may represent a novel therapeutic target for the development of new agents against cancer.

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Helianthus tuberosus

Lim¹

2014

Edible Medicinal and Non Medicinal Plants

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Stressed Jerusalem artichoke tubers (Helianthus tuberosus L.) excrete a protein fraction with specific cytotoxicity on plant and animal tumour cell

Cited by 14 publications

References 33 publications

The DING family of proteins: ubiquitous in eukaryotes, but where are the genes?

The DING family of proteins: ubiquitous in eukaryotes, but where are the genes?

Patatin-like PLA<sub>2</sub> with cytotoxicity against mammalian and plant tumour cells

Helianthus tuberosus

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Stressed Jerusalem artichoke tubers (Helianthus tuberosus L.) excrete a protein fraction with specific cytotoxicity on plant and animal tumour cell

Cited by 14 publications

References 33 publications

The DING family of proteins: ubiquitous in eukaryotes, but where are the genes?

The DING family of proteins: ubiquitous in eukaryotes, but where are the genes?

Patatin-like PLA&lt;sub&gt;2&lt;/sub&gt; with cytotoxicity against mammalian and plant tumour cells

Helianthus tuberosus

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Product

Resources

About

Patatin-like PLA<sub>2</sub> with cytotoxicity against mammalian and plant tumour cells