Abstract:Excretion of urinary proteins (UP) is an important marker for the evaluation of patients with progressive renal disease. In order to analyze quantitative and qualitative variability of UP in relation to physical activity, we used standardized stress tolerance test and SDS-PAGE. Five urine samples were obtained from each patient at rest, during ordinary daily activity and after physical stress. Determination of total proteins was performed using Meulman's classic method with sulfosalicylic acid. UP were separat… Show more
“…Before the treatment of a child with proteinuria, functional (orthostatic) proteinuria should be excluded [ 2 ] [ 3 ] [ 4 ] [ 5 ]. It is characterised by excretion of proteins only in postural position.…”
Section: Introductionmentioning
confidence: 99%
“…If this processing shows normal results, only annual audits are sufficient. SDS-PAG electrophoresis is an elegant method where the presence of Apolipoprotein A1 fraction (APO-A1) with a molecular mass of 28 KD is a pathognomonic sign for orthostatic proteinuria [ 2 ] [ 3 ].…”
Section: Introductionmentioning
confidence: 99%
“…It is a method that was developed during the 90s of the last century. The advantage is that a panoramic view of all proteins is obtained, so a clear classification of the type of proteinuria is possible [ 1 ] [ 2 ] [ 3 ] [ 17 ] [ 18 ] [ 19 ] [ 20 ]. It is relatively fast and inexpensive method; it is possible to analyse multiple samples, especially using the Phast system [ 21 ].…”
BACKGROUND:Proteinuria, in addition to haematuria, is the most important laboratory parameter in patients with nephro-urological diseases. Low molecular weight proteinuria (LMWP) is of particular importance because some diseases genetic and tubulointerstitial are diagnosed based on its presence.AIM:The purpose of this study is to describe the clinical features, the course and outcome of pediatric patients with a renal disease associated with LMWP.MATERIAL AND METHODS:This retrospective observational study included 250 pediatric patients with various kidney diseases in which the type of proteinuria was defined by 4-20% gradient gel sodium dodecyl sulphate polyacrylamide gel (SDS-PAG) electrophoresis.RESULTS:Isolated LMWP was detected in 12% of patients, while mixed glomerulotubular proteinuria was detected in 18% of patients. It was detected in all patients with the Dent-1/2 disease, Lowe’s syndrome and secondary Fanconi syndrome. Transient LMWP was also detected in a series of 12 patients with distal renal tubular acidosis. In patients with nephrotic syndrome, it was associated with corticoresistence and unfavourable clinical course.CONCLUSION:This study contributes to the understanding of the clinical spectrum of various kidney diseases associated with LMWP, their natural course, and the effect of therapy.
“…Before the treatment of a child with proteinuria, functional (orthostatic) proteinuria should be excluded [ 2 ] [ 3 ] [ 4 ] [ 5 ]. It is characterised by excretion of proteins only in postural position.…”
Section: Introductionmentioning
confidence: 99%
“…If this processing shows normal results, only annual audits are sufficient. SDS-PAG electrophoresis is an elegant method where the presence of Apolipoprotein A1 fraction (APO-A1) with a molecular mass of 28 KD is a pathognomonic sign for orthostatic proteinuria [ 2 ] [ 3 ].…”
Section: Introductionmentioning
confidence: 99%
“…It is a method that was developed during the 90s of the last century. The advantage is that a panoramic view of all proteins is obtained, so a clear classification of the type of proteinuria is possible [ 1 ] [ 2 ] [ 3 ] [ 17 ] [ 18 ] [ 19 ] [ 20 ]. It is relatively fast and inexpensive method; it is possible to analyse multiple samples, especially using the Phast system [ 21 ].…”
BACKGROUND:Proteinuria, in addition to haematuria, is the most important laboratory parameter in patients with nephro-urological diseases. Low molecular weight proteinuria (LMWP) is of particular importance because some diseases genetic and tubulointerstitial are diagnosed based on its presence.AIM:The purpose of this study is to describe the clinical features, the course and outcome of pediatric patients with a renal disease associated with LMWP.MATERIAL AND METHODS:This retrospective observational study included 250 pediatric patients with various kidney diseases in which the type of proteinuria was defined by 4-20% gradient gel sodium dodecyl sulphate polyacrylamide gel (SDS-PAG) electrophoresis.RESULTS:Isolated LMWP was detected in 12% of patients, while mixed glomerulotubular proteinuria was detected in 18% of patients. It was detected in all patients with the Dent-1/2 disease, Lowe’s syndrome and secondary Fanconi syndrome. Transient LMWP was also detected in a series of 12 patients with distal renal tubular acidosis. In patients with nephrotic syndrome, it was associated with corticoresistence and unfavourable clinical course.CONCLUSION:This study contributes to the understanding of the clinical spectrum of various kidney diseases associated with LMWP, their natural course, and the effect of therapy.
“…We have successfully used it for differentiation of glomerular from non-glomerular hematurias and for analysis of functional proteinurias (orthostatic, exercise induced proteinuria). [19,20,21] Analysis of the elepherograms enables pan-oramic investigation of various fractions according to their molecular weight and more subtle characterization of proteinuria (e.g. non selective glomerular with incomplete tubular proteinuria).…”
Distal renal tubular acidosis (dRTA) (MIM #267300, #602722 and #179800) is a rare inherited tubulopathy characterized by the inability of the distal tubule to acidify the urine with consecutive systemic acidosis. The clinical features include polyuria, polydipsia, poor appetite, failure to thrive, short stature and rickets. Prominent biochemical features are hypokalemia, hypercalciuria and hypocitraturia. There are reports on patients who presented with unusual biochemical features such as low molecular proteinuria, hypophosphatemia, hypouricemia, generalized hyperaminioaciduria, hyperoxaluria and other making diagnostic confusion to the clinicians. In this work, we report on a series of 8 children with clinically, biochemically and genetically proven dRTA who present with low molecular proteinuria at the disease onset. With metabolic compensation of the disease, there was complete resolution of the low molecular weight protenuria and other proximal tubular abnormalities in all children. Late recognition of the disease with long standing hypokalemia and acidosis may result in abnormal expression and function of the transporters in the proximal tubules. Sodium dodecyl sulphate polyacrylamide gel electrophoeresis is an accurate method for detection and follow up of patients with low molecular weight proteinuria.
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