Stress Tolerance and Glucose Insensitive Phenotypes in Arabidopsis Overexpressing theCpMYB10Transcription Factor Gene

Abstract

The resurrection plant Craterostigma plantagineum has the ability to survive complete dehydration. In an attempt to further understand desiccation tolerance in this plant, the CpMYB10 transcription factor gene was functionally characterized. CpMYB10 is rapidly induced by dehydration and abscisic acid (ABA) treatments in leaves and roots, but no expression was detected in fully hydrated tissues. Electrophoretic mobility shift assay experiments showed binding of rCpMYB10 to specific mybRE elements within the LEA Cp11-24 and CpMYB10 promoters. Localization of CpMYB10 transcript by in situ reverse transcription-PCR reactions showed expression in vascular tissues, parenchyma, and epidermis both in leaves and roots in response to ABA. Transgenic Arabidopsis plants transformed with CpMYB10 promoter fused to GUS gene showed reporter expression under ABA and stress conditions in several organs. Overexpression of CpMYB10 cDNA in Arabidopsis led to desiccation and salt tolerance of transgenics lines. Interestingly, it was found that plants overexpressing CpMYB10 exhibited Glc-insensitive and ABA hypersensitive phenotypes. Therefore, our results indicate that CpMYB10 in Arabidopsis is mediating stress tolerance and altering ABA and Glc signaling responses.

Relationship between water use efficiency (WUE) and production of different wheat genotypes at soil water deficit

“…Plants have evolved a wide range of defense systems to survive continuous assault by an arsenal of biotic attacks, constantly changing weather and other environmental conditions [35,41,42,45,55,56,61,63,69,73,75,77]. Unlocking the physiological and molecular basis for the plasticity of plant defending metabolism not only provides access to a largely untapped resource of genes and selection markers for breeding enhanced stress tolerance in crops but also ensures improved food security and agricultural sustainable development worldwide [5,8,10,19,32,44,48,52,62].…”

“…Just 1 year ago, European Commission, who once kept conservative to biotechnological breeding, constructed a big project: Plants for the Future: a European vision for Plant Biotechnology towards 2025, in which much is involved in resistance drought [14]. Many advances in relation to this hot topic, including molecular mechanism of anti-drought and corresponding molecular breeding have taken place [28,33,34,39,44,47,50,53,[55][56]63,[72][73][74][75]78]. Although the obtained transgenic crops (mainly, wheat) by different types of gene technology all exhibit drought resistance to some extent, they have many shortfalls related to agronomical performance and/or development [47,49,53,62,66,68,74].…”

Changes of some anti-oxidative physiological indices under soil water deficits among 10 wheat (Triticum aestivum L.) genotypes at tillering stage

“…Just 2 years ago, European Commission, who once kept conservative to biotechnological breeding, constructed a big project: Plants for the Future in which much is involved in resistance drought [5]. Many advances in relation to this hot topic, including molecular mechanism of antidrought and corresponding molecular breeding have taken place [4,10,16,17,21,[23][24][25]30,31,35,37,39,42,48,[55][56][57][58][59]. Although the obtained transgenic crops (mainly, wheat) by different types of gene technology all exhibit resistance drought to some extent, they have many shortfalls related to agronomical performance and/or development [1,16,17,21,24,30,39,49,56].…”

Raising salinity tolerant rice: recent progress and future perspectives

“…(Riano-Pachon et al, 2007). In Arabidopsis, transcription factors belonging to various subfamilies such as DREB1A and DREB2A of AP2 family (Liu et al, 1998), AREB1, AREB2 and AREB3 of bZIP family (Uno et al, 2000), Atmyb2, CpMYB10 and BOS1 of MYB family (Urao et al, 1993;Mengiste et al, 2003;Villalobos et al, 2004), RD26, ANAC019, ANAC055 and ANAC072 of NAC family Tran et al, 2004), and zinc finger proteins such as AZF1, AZF2, AZF3, STZ and ZPT2-3 (Sugano et al, 2003;Sakamoto et al, 2004) have been implicated in plant stress responses. Multiple TFs required for transcriptome reprogramming under abiotic stresses have been identified and functionally analyzed.…”