Stress, Sublethal Injury, Resuscitation, and Virulence of Bacterial Foodborne Pathogens

Wesche, Alissa M.; Gurtler, Joshua B.; Marks, Bradley P.; Ryser, Elliot T.

doi:10.4315/0362-028x-72.5.1121

Cited by 413 publications

(300 citation statements)

References 197 publications

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“…Those injured cells may recover and then grow under proper conditions possibly to cause some microbiological problem in food safety, human health and manufacturing of industrial products. In practical microbiological tests, therefore, the detection of such injured microorganisms have recently been concerned and different methods for the detection of injured microbes based upon their own principles have been developed so far e.g., Mossell and van Netten, 1984;Foegeding and Ray, 1992;Wesche et al, 2009;Tsuchido, 2013 . Those methods can be in general divided into cultivation and non-cultivation techniques.…”

Section: Communicationmentioning

confidence: 99%

A Novel Double Subculture Method and Its Theory for the Enumeration of Injured Cells in Stressed Microbial Population

Tsuchido

2017

Biocontrol Sci.

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A novel double subculture method, termed DiVSaL Differential Viabilities between Solid and Liquid media method, for the enumeration of injured cell population of a microorganism, which occurs after some sublethal to lethal treatment, was proposed. In this method injured cells were enumerated as the differential value between viabilities determined with two different techniques, the conventional plate counting using a solid agar medium and the growth delay analysis using a liquid medium. In the former technique, the viable cell number is obtained as colony forming unit CFU formed on an agar medium where sublethally injured cells are as much rescued as possible. In the latter technique, on the other hand, the integrated viability defined by Takano and Tsuchido 1982 is introduced and is calculated from the growth delay of a stressed population, referred to unstressed one. For the growth delay analysis, in this paper, not only the original theoretical model, where the specific growth rate and therefore the defined G 10 value does not change after the exposure to a stress treatment, but also a novel modified theory, where the parameter changes, is proposed. On the theoretical background, this DiVSaL method as a double subculture method can be used to enumerate the injured cells without selection by addition of some inhibitor or by nutritional shortage.

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Section: Communicationmentioning

confidence: 99%

A Novel Double Subculture Method and Its Theory for the Enumeration of Injured Cells in Stressed Microbial Population

Tsuchido

2017

Biocontrol Sci.

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“…Pathogens in foods are also frequently exposed to stresses with varying magnitudes [6]. Stresses to these microorganisms in foods during processing include physical stresses, such as heat, high pressure, desiccation, and irradiation, chemical stresses, such as acids, salts, and oxidants, and biological stresses, such as microbial antagonism [7].…”

Section: Stresses Encountered By Foodborne Pathogensmentioning

confidence: 99%

Stress Responses of Foodborne Pathogens and Implications in Food Safety

Zhao¹

2017

J Food Microbiol Saf Hyg

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Some microorganisms can induce adaptive responses to environmental stresses, which can enhance their tolerance to these stresses and may promote persistence under adverse conditions. Stress responses of foodborne pathogens can have profound effects on their survival in foods. Additionally, exposure to one sublethal stress may produce a spectrum of adaptive responses, cross-protecting microorganisms against multiple stresses. Understanding the mechanisms underlying the microbial responses to different stresses will improve the effective use of intervention strategies to inhibit the survival of pathogens in foods.

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“…The mechanism of cinnamaldehyde that triggered the cellular recovery from VBNC state is unknown and still needs further investigation. Wesche et al [38] explained that the alternate sigma factor, when triggered in bacterial pathogens under stress, may induce cellular survival or cross protection, enabling cells to resist other environmental stresses. It is possible that cinnamaldehyde could have triggered this or similar stress-response mechanisms, leading to cellular "hardening," which could have promoted survival at the 4 ∘ C storage temperature.…”

Section: Parahaemolyticus Inactivation In Shrimp With Low Density mentioning

confidence: 99%

Inactivation Kinetics ofVibrio parahaemolyticuson Sand Shrimp(Metapenaeus ensis)by Cinnamaldehyde at 4°C

Zhang

Dong

Ross

2017

Journal of Food Quality

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Sand shrimp (Metapenaeus ensis), shrimp shell, and shrimp meat were inoculated with a three-strain cocktail of Vibrio parahaemolyticus with or without the natural antimicrobial cinnamaldehyde (2.5 mg/ml) and were, then, stored at 4 ∘ C for up to 25 days and 18 inactivation curves were obtained. V. parahaemolyticus were inactivated down to the minimum level of detection (2.48 log CFU/g) on thiosulfate citrate bile salts sucrose agar (TCBS) plates within 7 and 10 days with low and high densities of V. parahaemolyticus inoculation, 4.5 log CFU/g and 8.2 log CFU/g, respectively. With adding cinnamaldehyde, the inactivation process of V. parahaemolyticus with low populations, 4.5 log CFU/g, lasted for only 4 days. Therefore, cinnamaldehyde inactivated cells faster as expected. However, unexpectedly, in shrimp meat cases, cells have much more persistence of over even 25 days before entering the minimum level of detection both with and without cinnamaldehyde treatment. Therefore, a hypothesis was formed that when cells kept in cold environments (4 ∘ C) after several days recovered to up to 10 3 -10 4 CFU/g towards the end of the experiments and with starvation (shell and shrimp studies), cells might render a viable but nonculturable (VBNC) state.

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Stress, Sublethal Injury, Resuscitation, and Virulence of Bacterial Foodborne Pathogens

Cited by 413 publications

References 197 publications

A Novel Double Subculture Method and Its Theory for the Enumeration of Injured Cells in Stressed Microbial Population

A Novel Double Subculture Method and Its Theory for the Enumeration of Injured Cells in Stressed Microbial Population

Stress Responses of Foodborne Pathogens and Implications in Food Safety

Inactivation Kinetics ofVibrio parahaemolyticuson Sand Shrimp(Metapenaeus ensis)by Cinnamaldehyde at 4°C

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