Stress Responsive Proteins Are Actively Regulated during Rice (Oryza sativa) Embryogenesis as Indicated by Quantitative Proteomics Analysis

Zi, Jian; Zhang, Jiyuan; Wang, Quanhui; Zhou, Baojin; Zhong, Junyan; Zhang, Chaoliang; Qiu, Xuemei; Wen, Bo; Zhang, Shenyan; Fu, Xiqin; Lin, Liang; Liu, Siqi

doi:10.1371/journal.pone.0074229

Cited by 39 publications

(34 citation statements)

References 48 publications

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“…These cellular responses, helping in the prevention or alleviation of cellular damage, the re-establishment of homeostatic conditions and the resumption of growth, often mimics certain normal stages of development2930. Thus, there is a broader overlapping set of genes and their cognate proteins involved in developmental processes and stress responses which are driven by systematic regulation of gene expression31323334. Our data showed that gene OsSGL participated in the regulation of both stress-tolerance and yield component traits in rice.…”

Section: Discussionmentioning

confidence: 64%

OsSGL, a novel pleiotropic stress-related gene enhances grain length and yield in rice

Wang

et al. 2016

Sci Rep

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Abiotic stress seriously affects the yield of rice (Oryza sativa L.). Grain yield in rice is multiplicatively determined by the number of panicles, number of grains per panicle, and grain weight. Here, we describe the molecular and functional characterization of STRESS_tolerance and GRAIN_LENGTH (OsSGL), a rice gene strongly up-regulated by a wide spectrum of abiotic stresses. OsSGL encodes a putative member of the DUF1645 protein family of unknown function. Overexpression of OsSGL significantly altered certain development processes greatly and positively affecting an array of traits in transgenic rice plants, including increased grain length, grain weight and grain number per panicle, resulting in a significant increase in yield. Microscopical analysis showed that the enhanced OsSGL expression promoted cell division and grain filling. Microarray and quantitative real-time PCR (qRT-PCR) analyses revealed that a large number of genes involved in stress-response, cell cycle and cytokinin signaling processes were induced or suppressed in OsSGL-overexpressing plants. Together, our results suggest that OsSGL may regulate stress-tolerance and cell growth by acting via a cytokinin signaling pathway. This study not only contributes to our understanding of the underlying mechanism regulating rice stress-tolerance and grain length, but also provides a strategy for tailor-made crop yield improvement.

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Section: Discussionmentioning

confidence: 64%

OsSGL, a novel pleiotropic stress-related gene enhances grain length and yield in rice

Wang

et al. 2016

Sci Rep

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“…The proteins were isolated using trichloroacetic acid (TCA) [ 23 ]. About 20 mg of pooled protein from control or salt stress treated samples was labeled with iTRAQ reagents according to the manufacturer’s instructions (iTRAQ Reagents Multiplex kit, Applied Biosystems/MDS Sciex, Foster City, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

Quantitative Proteomic Analysis of the Rice (Oryza sativa L.) Salt Response

Lan

Fang

et al. 2015

PLoS ONE

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Salt stress is one of most serious limiting factors for crop growth and production. An isobaric Tags for Relative and Absolute Quantitation (iTRAQ) approach was used to analyze proteomic changes in rice shoots under salt stress in this study. A total of 56 proteins were significantly altered and 16 of them were enriched in the pathways of photosynthesis, antioxidant and oxidative phosphorylation. Among these 16 proteins, peroxiredoxin Q and photosystem I subunit D were up-regulated, while thioredoxin M-like, thioredoxin x, thioredoxin peroxidase, glutathione S-transferase F3, PSI subunit H, light-harvesting antenna complex I subunits, chloroplast chaperonin, vacuolar ATP synthase subunit H, and ATP synthase delta chain were down-regulated. Moreover, physiological data including total antioxidant capacity, peroxiredoxin activity, chlorophyll a/b content, glutathione S-transferase activity, reduced glutathione content and ATPase activity were consistent with changes in the levels of these proteins. The levels of the mRNAs encoding these proteins were also analyzed by real-time quantitative reverse transcription PCR, and approximately 86% of the results were consistent with the iTRAQ data. Importantly, our data suggest the important role of PSI in balancing energy supply and ROS generation under salt stress. This study provides information for an improved understanding of the function of photosynthesis and PSI in the salt-stress response of rice.

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“…Recently, an alternative approach has been developed using isobaric tag for relative and absolute quantitation (iTRAQ) as a mass spectrometry-based quantitative technology; this technique overcomes some of the limitations of 2-DE, especially for multiple samples, and allows identification of a greater number of proteins to provide more reliable quantitative information [28, 29]. The advantages of iTRAQ technology have been exploited to identify and quantify 2165 proteins in developing rice embryos [30] and 1815 proteins in wheat grains [31]. …”

Section: Introductionmentioning

confidence: 99%

Proteomic analysis of maize grain development using iTRAQ reveals temporal programs of diverse metabolic processes

Dong

et al. 2016

BMC Plant Biol

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BackgroundGrain development in maize is an essential process in the plant’s life cycle and is vital for use of the plant as a crop for animals and humans. However, little is known regarding the protein regulatory networks that control grain development. Here, isobaric tag for relative and absolute quantification (iTRAQ) technology was used to analyze temporal changes in protein expression during maize grain development.ResultsMaize grain proteins and changes in protein expression at eight developmental stages from 3 to 50 d after pollination (DAP) were performed using iTRAQ-based proteomics. Overall, 4751 proteins were identified; 2639 of these were quantified and 1235 showed at least 1.5-fold changes in expression levels at different developmental stages and were identified as differentially expressed proteins (DEPs). The DEPs were involved in different cellular and metabolic processes with a preferential distribution to protein synthesis/destination and metabolism categories. A K-means clustering analysis revealed coordinated protein expression associated with different functional categories/subcategories at different development stages.ConclusionsOur results revealed developing maize grain display different proteomic characteristics at distinct stages, such as numerous DEPs for cell growth/division were highly expressed during early stages, whereas those for starch biosynthesis and defense/stress accumulated in middle and late stages, respectively. We also observed coordinated expression of multiple proteins of the antioxidant system, which are essential for the maintenance of reactive oxygen species (ROS) homeostasis during grain development. Particularly, some DEPs, such as zinc metallothionein class II, pyruvate orthophosphate dikinase (PPDK) and 14-3-3 proteins, undergo major changes in expression at specific developmental stages, suggesting their roles in maize grain development. These results provide a valuable resource for analyzing protein function on a global scale and also provide new insights into the potential protein regulatory networks that control grain yield and quality.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-016-0878-1) contains supplementary material, which is available to authorized users.

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Stress Responsive Proteins Are Actively Regulated during Rice (Oryza sativa) Embryogenesis as Indicated by Quantitative Proteomics Analysis

Cited by 39 publications

References 48 publications

OsSGL, a novel pleiotropic stress-related gene enhances grain length and yield in rice

OsSGL, a novel pleiotropic stress-related gene enhances grain length and yield in rice

Quantitative Proteomic Analysis of the Rice (Oryza sativa L.) Salt Response

Proteomic analysis of maize grain development using iTRAQ reveals temporal programs of diverse metabolic processes

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