Stress responses to warming in Japanese flounder (Paralichthys olivaceus) from different environmental scenarios

Han, Ping; Qiao, Yingjie; He, Jianzhong; Wang, Xubo

doi:10.1016/j.scitotenv.2023.165341

Cited by 8 publications

(4 citation statements)

References 74 publications

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“…The detection of GPT, GOT, and AKP activities is a common way to diagnose damage to the liver (41). GPT and GOT, as aminotransferases, can catalyze the redistribution of nitrogen between amino acids and corresponding oxoacids, thereby regulating protein metabolism and gluconeogenesis (20). In our study, plasma GPT and GOT activities were significantly increased to the highest levels at 24 h after A. hydrophila infection and decreased gradually at the later infective stages, indicating that A. hydrophila infection led to severe hepatic damage at the initial stage of infection.…”

Section: Plasma Biochemical Indices Changed After Infectionsupporting

confidence: 50%

“…In our study, plasma GPT and GOT activities were significantly increased to the highest levels at 24 h after A. hydrophila infection and decreased gradually at the later infective stages, indicating that A. hydrophila infection led to severe hepatic damage at the initial stage of infection. Similarly, GPT and GOT activities were elevated in Japanese flounder in response to heat stress (20) and in crucian carp after A. veronii challenge (21).…”

Section: Plasma Biochemical Indices Changed After Infectionmentioning

confidence: 87%

“…For example, glutamate pyruvate transaminase (GPT) and glutamic oxalacetic transaminase (GOT), as ubiquitous transaminases, are two clinical biomarkers of hepatic health. These two enzymes, generally existing in hepatocytes, are secreted into the blood when the permeability of the hepatic cell membrane becomes damaged (20). Alkaline phosphatase (AKP), existing in macrophage lysosomes, has important functions in immunity, as it can mitigate the negative effects of lipopolysaccharides and alleviate inflammation (21).…”

Section: Introductionmentioning

confidence: 99%

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Integrated time-series biochemical, transcriptomic, and metabolomic analyses reveal key metabolites and signaling pathways in the liver of the Chinese soft-shelled turtle (Pelodiscus sinensis) against Aeromonas hydrophila infection

Ji,

Chen,

Zhu

et al. 2024

Front. Immunol.

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IntroductionAeromonas hydrophila, a bacterium widely distributed in the natural environment, causes multiple diseases in various animals. Exploring the mechanism of the host defense against A. hydrophila can help develop efficient strategies against Aeromonas infection.MethodsHerein, we investigated the temporal influence of A. hydrophila on the Chinese soft-shelled turtle, an economically important species, at the biochemical, transcriptomic, and metabolomic levels. Plasma parameters were detected with the test kits. Transcriptome and metabolome were respectively applied to screen the differentially expressed genes and metabolites.ResultsThe contents or activities of these plasma parameters were significantly increased at 24 hpi and declined at 96 hpi, indicating that 24 and 96 hpi were two important time points during infection. Totals of 3121 and 274 differentially expressed genes (DEGs) from the transcriptome while 74 and 91 differentially abundant metabolites (DAMs) from the metabolome were detected at 24 and 96 hpi. The top DEGs at 24 hpi included Ccl2, Ccl3, Ccl4, Il1β, Il6, Il7, Il15, Tnf, and Tnfr1 while Zap70, Cd3g, Cd8a, Itk, Pik3r3, Cd247, Malt1, and Cd4 were the most abundant at 96 hpi. The predominant DAMs included O-phospho-L-serine, γ-Aminobutyric acid, orotate, L-tyrosine, and L-tryptophan at 24 hpi, as well as L-glutamic acid, L-arginine, glutathione, glutathione disulfide, and citric acid at 96 hpi.DiscussionThe combined analysis of DEGs and DAMs revealed that tryptophan metabolism, nicotinate and nicotinamide metabolism, as well as starch and sucrose metabolism, were the most important signaling pathways at the early infective stage while tyrosine metabolism, pyrimidine metabolism, as well as alanine, aspartate and glutamate metabolism were the most crucial pathways at the later stage. In general, our results indicated that the Chinese soft-shelled turtle displays stage-specific physiological responses to resist A. hydrophila infection.

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Section: Plasma Biochemical Indices Changed After Infectionsupporting

confidence: 50%

Section: Plasma Biochemical Indices Changed After Infectionmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 99%

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Integrated time-series biochemical, transcriptomic, and metabolomic analyses reveal key metabolites and signaling pathways in the liver of the Chinese soft-shelled turtle (Pelodiscus sinensis) against Aeromonas hydrophila infection

Ji,

Chen,

Zhu

et al. 2024

Front. Immunol.

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“…In our findings, serum levels of ALT and AST in PF-carp during heat stress showed no significant changes, suggesting that heat stress did not affect the dysfunction of the hepatopancreas. In addition, Japanese flounder (Paralichthys olivaceus) (Han et al, 2023), pikeperch (Sander lucioperca) (Chen, Y. et al, 2021), and largemouth bass (Micropterus salmoides) (Zhao et al, 2022) often show damage to their tissues after experiencing heat stress.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis in hepatopancreases reveals the response of domesticated common carp to a high-temperature environment in the agricultural heritage rice–fish system

Cheng,

Li,

Kumilamba

et al. 2023

Front. Physiol.

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Qingtian paddy field carp (PF-carp) is a local carp cultivated in the paddy field of Qingtian, Zhejiang. This rice–fish co-culture system has been recognized as one of the Globally Important Agriculture Heritage Systems (GIAHS). PF-carp has been acclimatized to the high-temperature environment of shallow paddy fields after several centuries of domestication. To reveal the physiological and molecular regulatory mechanisms of PF-carp, we chose to use 28°C as the control group and 34°C as the treatment group. We measured biochemical parameters in their serum and hepatopancreases and also performed transcriptome sequencing analysis. Compared with the control group, the serum levels of malondialdehyde (MDA), glucose (GLU), glutathione peroxidase (GSH-Px), catalase (CAT), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) show no significant change. In addition, superoxide dismutase (SOD), GSH-Px, and CAT also show no significant change in hepatopancreases. We identified 1,253 differentially expressed genes (DEGs), and their pathway analysis revealed that heat stress affected AMPK signaling pathway, protein export, and other biological processes. It is worth noting that protein processing in the endoplasmic reticulum (ER) was the most significantly enriched pathway identified by the Kyoto Encyclopedia of Genes and Genomes (KEGG) and gene set enrichment analysis (GSEA). Significantly higher levels of HSP40, HSP70, HSP90, and other ubiquitin ligase-related genes were upregulated. In summary, heat stress did not lead to tissue damage, inflammation, oxidative stress, and ER stress in the hepatopancreases of PF-carp. This study provides valuable insights into the adaptation mechanism of this species to the high-temperature environment of paddy fields.

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Stress responses to warming in Japanese flounder (Paralichthys olivaceus) from different environmental scenarios

Cited by 8 publications

References 74 publications

Integrated time-series biochemical, transcriptomic, and metabolomic analyses reveal key metabolites and signaling pathways in the liver of the Chinese soft-shelled turtle (Pelodiscus sinensis) against Aeromonas hydrophila infection

Integrated time-series biochemical, transcriptomic, and metabolomic analyses reveal key metabolites and signaling pathways in the liver of the Chinese soft-shelled turtle (Pelodiscus sinensis) against Aeromonas hydrophila infection

Transcriptome analysis in hepatopancreases reveals the response of domesticated common carp to a high-temperature environment in the agricultural heritage rice–fish system

A study on the hepatic response to heat stress in Gymnocypris eckloni through an approach combining metabolomic and transcriptomic profiling

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