Stress responses to phenol in Arabidopsis and transcriptional changes revealed by microarray analysis

Xu, Jing; Su, Zhonggen; Chen, Chen; Han, Ho Jae; Zhu, Bo; Fu, Xin; Wang, Zhao; Jin, Xin; Wu, Aiming; Yao, Quan‐Hong

doi:10.1007/s00425-011-1498-5

Cited by 16 publications

(4 citation statements)

References 48 publications

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“…6a-d). Similar results were found in homolog tobacco MXC-related genes identified in Arabidopsis seedlings which were also upregulated after exposure to phenol and acid-extractable organic fraction (Xu et al 2012;Widdup et al 2015). Based in our results, we argue that phenol treatment would work as an alternative regulatory signal masking the circadian regulation.…”

Section: Aq3supporting

confidence: 88%

Identification and characterization of key circadian clock genes of tobacco hairy roots: putative regulatory role in xenobiotic metabolism

Alderete

Guido

Agostini

et al. 2017

Environ Sci Pollut Res

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The circadian clock is an endogenous system that allows organisms to daily adapt and optimize their physiology and metabolism. We studied the key circadian clock gene (CCG) orthologs in Nicotiana tabacum seedlings and in hairy root cultures (HRC). Putative genes involved in the metabolism of xenobiotic compounds (MXC) were selected and their expression profiles were also analyzed. Seedlings and HRC displayed similar diurnal variations in the expression profiles for the CCG examined under control conditions (CC). MXC-related genes also showed daily fluctuations with specific peaks of expression. However, when HRC were under phenol treatment (PT), the expression patterns of the clock and MXC-related genes were significantly affected. In 2-week-old HRC, PT downregulated the expression of NtLHY, NtTOC1, and NtPRR9 while NtFKF1 and NtGI genes were upregulated by phenol. In 3-week-old HRC, PT also downregulated the expression of all CCG analyzed and NtTOC1 was the most affected. Following PT, the expression of the MXC-related genes was upregulated or displayed an anti-phasic expression profile compared to the expression under CC. Our studies thus provide a glimpse of the circadian expression of clock genes in tobacco and the use of HRC as a convenient system to study plant responses to xenobiotic stresses.

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Section: Aq3supporting

confidence: 88%

Identification and characterization of key circadian clock genes of tobacco hairy roots: putative regulatory role in xenobiotic metabolism

Alderete

Guido

Agostini

et al. 2017

Environ Sci Pollut Res

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“…Both APx and glutathione peroxidase activity changed in the same way in Arabidopsis thaliana plants exposed to 2,3,7,8-tetrachlorinated dibenzo- p -dioxin [ 69 ]. A similar situation concerning APx and POx was also found in phenol-treated Arabidopsis thaliana plants [ 70 ]. It is possible that the decline in APx and POx activities observed at the maximum sewage sludge dose (10.8g) indicates that the production of toxic ROS may have overwhelmed the plant antioxidant systems at this dose, causing the observed damage to tissue structures.…”

Section: Discussionsupporting

confidence: 71%

The Different Physiological and Antioxidative Responses of Zucchini and Cucumber to Sewage Sludge Application

Wyrwicka

Urbaniak

2016

PLoS ONE

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The present study investigates the effect of soil amended with sewage sludge on oxidative changes in zucchini and cucumber plants (Cucurbitaceae) and the consequent activation of their antioxidative systems and detoxification mechanisms. The plants were grown in pots containing soil amended with three concentrations of sewage sludge (1.8 g, 5.4 g and 10.8 g per pot), while controls were potted with vegetable soil. The activities of three antioxidative enzymes, ascorbate peroxidase (APx), catalase (CAT) and guaiacol peroxidase (POx), were assessed, as well as of the detoxifying enzyme S-glutathione transferase (GST). Lipid peroxidation was evaluated by measuring the extent of oxidative damage; α-tocopherol content, the main lipophilic antioxidant, was also measured. Visible symptoms of leaf blade damage after sewage sludge application occurred only on the zucchini plants. The zucchini and cucumber plants showed a range of enzymatic antioxidant responses to sewage sludge application. While APx and POx activities increased significantly with increasing sludge concentration in the zucchini plants, they decreased in the cucumber plants. Moreover, although the activity of these enzymes increased gradually with increasing doses of sewage sludge, these levels fell at the highest dose. An inverse relationship between peroxidases activity and CAT activity was observed in both investigated plant species. In contrast, although GST activity increased progressively with sludge concentration in both the zucchini and cucumber leaves, the increase in GST activity was greater in the zucchini plants, being visible at the lowest dose used. The results indicate that signs of sewage sludge toxicity were greater in zucchini than cucumber, and its defense reactions were mainly associated with increases in APx, POx and GST activity.

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“…We then compared our sets of DEGs to other transcriptome analyses performed to study the effect of various xenobiotics, such as aluminum [ 37 ], atrazine [ 38 ], benzoxazolin-2(3H)-one [ 39 ], cadmium [ 40 ], polychlorinated biphenyl [ 41 ], phenol [ 42 ] selenium [ 43 ] and trinitrotoluene [ 44 ]. Overall, 77.7 % (363/467) of DEGs were shared between the xenobiotic transcriptome datasets.…”

Section: Resultsmentioning

confidence: 99%

Unraveling the early molecular and physiological mechanisms involved in response to phenanthrene exposure

et al. 2016

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BackgroundHigher plants have to cope with increasing concentrations of pollutants of both natural and anthropogenic origin. Given their capacity to concentrate and metabolize various compounds including pollutants, plants can be used to treat environmental problems - a process called phytoremediation. However, the molecular mechanisms underlying the stabilization, the extraction, the accumulation and partial or complete degradation of pollutants by plants remain poorly understood.ResultsHere, we determined the molecular events involved in the early plant response to phenanthrene, used as a model of polycyclic aromatic hydrocarbons. A transcriptomic and a metabolic analysis strongly suggest that energy availability is the crucial limiting factor leading to high and rapid transcriptional reprogramming that can ultimately lead to death. We show that the accumulation of phenanthrene in leaves inhibits electron transfer and photosynthesis within a few minutes, probably disrupting energy transformation.ConclusionThis kinetic analysis improved the resolution of the transcriptome in the initial plant response to phenanthrene, identifying genes that are involved in primary processes set up to sense and detoxify this pollutant but also in molecular mechanisms used by the plant to cope with such harmful stress. The identification of first events involved in plant response to phenanthrene is a key step in the selection of candidates for further functional characterization, with the prospect of engineering efficient ecological detoxification systems for polycyclic aromatic hydrocarbons.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3133-0) contains supplementary material, which is available to authorized users.

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Stress responses to phenol in Arabidopsis and transcriptional changes revealed by microarray analysis

Cited by 16 publications

References 48 publications

Identification and characterization of key circadian clock genes of tobacco hairy roots: putative regulatory role in xenobiotic metabolism

Identification and characterization of key circadian clock genes of tobacco hairy roots: putative regulatory role in xenobiotic metabolism

The Different Physiological and Antioxidative Responses of Zucchini and Cucumber to Sewage Sludge Application

Unraveling the early molecular and physiological mechanisms involved in response to phenanthrene exposure

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